Soybean Isoflavones Suppress Basophil Degranulation in Vitro in Subjects with Food Allergy

2013 ◽  
Vol 131 (2) ◽  
pp. AB119
Author(s):  
Mohanapriya Kamalakannan ◽  
Matthew Stadler ◽  
Hugh A. Sampson ◽  
Madhan Masilamani
Keyword(s):  
Food Allergy ◽  
Soybean Isoflavones

scholarly journals Allergenic Characterization of New Mutant Forms of Pru p 3 as New Immunotherapy Vaccines

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
C. Gómez-Casado ◽  
M. Garrido-Arandia ◽  
P. Gamboa ◽  
N. Blanca-López ◽  
G. Canto ◽  
...  
Keyword(s):  
Food Allergy ◽  
Ex Vivo ◽  
Binding Capacity ◽  
Cross Reactivity ◽  
T Cell Epitopes ◽  
Native Form ◽  
Ige Binding ◽  
Pru P 3

Nowadays, treatment of food allergy only considered the avoidance of the specific food. However, the possibility of cross-reactivity makes this practice not very effective. Immunotherapy may exhibit as a good alternative to food allergy treatment. The use of hypoallergenic molecules with reduced IgE binding capacity but with ability to stimulate the immune system is a promising tool which could be developed for immunotherapy. In this study, three mutants of Pru p 3, the principal allergen of peach, were produced based on the described mimotope and T cell epitopes, by changing the specific residues to alanine, named asPru p 3.01, Pru p 3.02, andPru p 3.03.Pru p 3.01showed very similar allergenic activity as the wild type byin vitroassays. However,Pru p 3.02andPru p 3.03presented reduced IgE binding with respect to the native form, byin vitro,ex vivo,and in vivo assays. In addition,Pru p 3.03had affected the IgG4 binding capacity and presented a random circular dichroism, which was reflected in the nonrecognition by specific antibodies anti-Pru p 3. Nevertheless, bothPru p 3.02andPru p 3.03maintained the binding to IgG1 and their ability to activate T lymphocytes. Thus,Pru p 3.02andPru p 3.03could be good candidates for potential immunotherapy in peach-allergic patients.

scholarly journals Diagnosis of food allergy: History, examination and in vivo and in vitro tests

2014 ◽  
Vol 105 (1) ◽  
pp. 69
Author(s):  
D A Van der Spuy ◽  
A J Terblanche ◽  
S Karabus ◽  
M Kriel ◽  
A I Manjra ◽  
...  
Keyword(s):  
Food Allergy ◽  
In Vitro Tests

scholarly journals Flow cytometric analysis of in vitro activated basophils, specific IgE and skin tests in the diagnosis of pollen-associated food allergy

2005 ◽  
Vol 64B (1) ◽  
pp. 28-33 ◽  
Author(s):  
Didier G. Ebo ◽  
Margo M. Hagendorens ◽  
Chris H. Bridts ◽  
Annemie J. Schuerwegh ◽  
Luc S. De Clerck ◽  
...  
Keyword(s):  
Food Allergy ◽  
Flow Cytometric Analysis ◽  
Specific Ige ◽  
Skin Tests ◽  
Flow Cytometric

Probiotics Have a Different Immunomodulatory Potential in vitro versus ex vivo upon Oral Administration in Children with Food Allergy

2007 ◽  
Vol 143 (3) ◽  
pp. 237-244 ◽  
Author(s):  
A.E. Flinterman ◽  
E.F. Knol ◽  
A.G. van Ieperen-van Dijk ◽  
H.M. Timmerman ◽  
A.C. Knulst ◽  
...  
Keyword(s):  
Food Allergy ◽  
Oral Administration ◽  
Ex Vivo ◽  
Immunomodulatory Potential

In vitro testing for immunoglobulin E-mediated food allergies

1996 ◽  
Vol 115 (4) ◽  
pp. 312-318 ◽  
Author(s):  
Jacquelynne P. Corey ◽  
Anil Gungor
Keyword(s):  
Food Allergy ◽  
Immunoglobulin E ◽  
Diagnostic Methods ◽  
Food Allergies ◽  
Vitro Assay ◽  
Positive Screen ◽  
Specific Immunoglobulin E ◽  
Radioallergosorbent Test ◽  
Specific Immunoglobulin

The role of immunoglobulin E-mediated food allergy in subjects with allergic disorders, especially in patients with rhinitis and sinusitis, is underestimated by clinicians because of the initial attribution of these disorders to immediate-type hypersensitivity reactions. The difficulties of diagnosing food-related reactions have caused further delay in their recognition and incorporation into the daily practice of diagnosing food allergy. Two of the diagnostic methods for food allergy are the in vitro assay of total immunoglobulin E and the measurement of food-specific immunoglobulin E levels in serum with the radioallergosorbent test. Measurement of specific immunoglobulin E level is the most commonly used but also one of the most controversial techniques. We examined 123 patients with rhinitis who were referred to our otolaryngology/allergy clinic between January and April 1995. All patients received an initial radioallergosorbent test screen, which included milk. We determined the positive predictive value of this positive screen and, in particular, of a positive test for milk in the diagnosis of immunoglobulin E-mediated food allergies in these patients. Conclusions were based on comparison with the result of an additional radioallergosorbent test food panel consisting of eight common and two investigational food allergens. (Otolaryngol Head Neck Surg 1996;115:312-8.)

Novel in Vitro Method for Diagnosis of Food Allergy in Children: Utilization Of CD203c Expression in Basophils

2006 ◽  
Vol 117 (2) ◽  
pp. S40
Author(s):  
M. Nagao ◽  
R. Tokuda ◽  
Y. Noma ◽  
S. Nakayama ◽  
T. Fujisawa
Keyword(s):  
Food Allergy ◽  
In Vitro Method

scholarly journals Total IgE Distribution in Food Allergy Suspected Patients in Republic of Macedonia (2001-2011)

2015 ◽  
Vol 3 (2) ◽  
pp. 202-208
Author(s):  
Slavica Hristomanova Mitkovska ◽  
Dejan Trajkov ◽  
Aleksandar Petlichkovski ◽  
Olivija Efinska-Mladenovska ◽  
Mirko Spiroski
Keyword(s):  
Food Allergy ◽  
Human Genetics ◽  
Specific Ige ◽  
Average Concentration ◽  
Total Serum ◽  
Age Group ◽  
Total Ige ◽  
Serum Ige ◽  
Number Of Patients

BACKGROUND: IgE may be considered the hallmark of allergic disorders. It is easily detected in serum and can be measured as total IgE and as allergen-specific IgE. In fact, the serum IgE assay is used to diagnose an allergy.AIM: The aim of this study is to evaluate, investigate and present the distribution of total serum IgE levels, determined with UniCap system, in food-allergy suspected patients in a Republic of Macedonia.MATERIAL AND METHODS: In this study we analyzed retrospectively 8898 consecutive patients that were admitted for allergy testing at the Institute of Immunobiology and Human Genetics during the ten year period between 01.01.2001 and 01.01.2011. Total IgE levels in patient sera were detected with the in vitro system UniCAP100 (Pharmacia, Uppsala, Sweden).RESULTS: When we analyzed the number of patients according to the total IgE groups, we noted that most of the patients have normal levels of total IgE in serum. However, we also discovered a group of patients with elevated levels of total IgE that are greater than 200 kU/L. The average concentration of total serum IgE is higher in women in the age group 6 (6-7 years), followed by a steep decrease in the age group 9 (9-10 years), and after that the average concentrations of total IgE were mostly constant with the exception of a partial increase in the age group 21 (65-69 years). For men, the average serum concentrations of total IgE were highest in the age group of 6 (6-7 years), which was significantly higher than the average concentrations of total IgE in all other age groups.CONCLUSION: The large number of enrolled patients, a particular strength of this study, revealed that average concentrations of total IgE in men are higher than in women and that total IgE did not decrease with age. On the contrary, increased total IgE levels were found in patients aged 65 and 69 of both genders. We continue our work with analyses of the specific IgE antibodies values toward food and the correlation with total IgE values.

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