In vitro testing for immunoglobulin E-mediated food allergies

1996 ◽  
Vol 115 (4) ◽  
pp. 312-318 ◽  
Author(s):  
Jacquelynne P. Corey ◽  
Anil Gungor
Keyword(s):  
Food Allergy ◽  
Immunoglobulin E ◽  
Diagnostic Methods ◽  
Food Allergies ◽  
Vitro Assay ◽  
Positive Screen ◽  
Specific Immunoglobulin E ◽  
Radioallergosorbent Test ◽  
Specific Immunoglobulin

The role of immunoglobulin E-mediated food allergy in subjects with allergic disorders, especially in patients with rhinitis and sinusitis, is underestimated by clinicians because of the initial attribution of these disorders to immediate-type hypersensitivity reactions. The difficulties of diagnosing food-related reactions have caused further delay in their recognition and incorporation into the daily practice of diagnosing food allergy. Two of the diagnostic methods for food allergy are the in vitro assay of total immunoglobulin E and the measurement of food-specific immunoglobulin E levels in serum with the radioallergosorbent test. Measurement of specific immunoglobulin E level is the most commonly used but also one of the most controversial techniques. We examined 123 patients with rhinitis who were referred to our otolaryngology/allergy clinic between January and April 1995. All patients received an initial radioallergosorbent test screen, which included milk. We determined the positive predictive value of this positive screen and, in particular, of a positive test for milk in the diagnosis of immunoglobulin E-mediated food allergies in these patients. Conclusions were based on comparison with the result of an additional radioallergosorbent test food panel consisting of eight common and two investigational food allergens. (Otolaryngol Head Neck Surg 1996;115:312-8.)

Future Approaches to Food Allergy

PEDIATRICS ◽  
2003 ◽  
Vol 111 (Supplement_3) ◽  
pp. 1672-1680
Author(s):  
Anna Nowak-Wegrzyn
Keyword(s):  
Food Allergy ◽  
Immunoglobulin E ◽  
Fatal Outcome ◽  
Diagnostic Methods ◽  
Food Allergies ◽  
In Vitro Assays ◽  
Activation Markers ◽  
Treatment Of Food Allergy

Food allergy affects ∼2% of the general US population, and its prevalence seems to be increasing. Despite the potential for a fatal outcome, no definitive therapies are available for food allergy. This article reviews novel approaches for the diagnosis and treatment of food allergy. Improved diagnostic methods include more precise in vitro and in vivo tests for immunoglobulin E-mediated food allergies, in vitro assays for predicting development of oral tolerance, and novel noninvasive tests for cell-mediated food allergies such as patch testing, cytokine assays, and detection of eosinophil activation markers. Several promising novel immunomodulatory approaches to food allergy are discussed, including monoclonal anti-immunoglobulin E; probiotics; traditional Chinese medicine; and immunotherapy with modified food proteins, peptides, bacterial adjuvants, and immunostimulatory sequences.

scholarly journals Skin Prick Tests and Patch Tests in Children with Food Allergies.

2020 ◽  
pp. 1-4
Keyword(s):  
Immunoglobulin E ◽  
Skin Prick Testing ◽  
Food Challenge ◽  
Diagnostic Methods ◽  
Food Allergies ◽  
Sufficient Evidence ◽  
Patch Tests ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Skin Prick Tests

Abstract Diagnosing food allergies can be challenging for patients and health professionals. Standard diagnostic methods include skin prick testing, food-specific immunoglobulin E (IgE) and oral food challenge. There is no scientifically sufficient evidence for routine use of patch testing for food allergy evaluation in children.

Latex Hypersensitivity in Children: Clinical Presentation and Detection of Latex-Specific Immunoglobulin E

PEDIATRICS ◽  
1995 ◽  
Vol 95 (5) ◽  
pp. 693-699
Author(s):  
Pamela L. Kwittken ◽  
Sharon K. Sweinberg ◽  
Donald E. Campbell ◽  
Nicholas A. Pawlowski
Keyword(s):  
High Risk ◽  
Immunoglobulin E ◽  
High Risk Patient ◽  
Latex Allergy ◽  
Patient Profile ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin ◽  
Life Threatening ◽  
History Of

Objective. To better understand the clinical characteristics, diagnosis, and possible prevention of immediate hypersensitivity reactions to latex in a hospitalized, pediatric patient population. Methods. We performed a retrospective case analysis of the first 35 patients with latex allergy evaluated by our service over a 2-year period at our institution. Characteristics of patients and clinical reactions were analyzed and the presence of latex-specific immunoglobulin E was assessed using in vitro methods. In a limited group of Objective. To better understand the clinical premedication with steroids and antihistamines was evaluated for the prevention of latex allergic reactions. Results. The majority of our patients had life-threatening reactions. In previous reports, most pediatric patients underwent reactions in the perioperative period and belonged to two well-recognized "high-risk" patient groups (spina bifida and genitourinary malformations). In our series, 21 patients (60%) had reactions outside of the operating room setting, and 14 patients (40%) had primary diagnoses outside of the previously recognized "high-risk" groups. Many patients had a history of multiple surgical procedures, and a history of a surgical procedure in the first year of life was very common. A pre-existing clinical history of latex allergy was present in only 18 of the 35 patients, and a severe or life-threatening allergic reaction was the presenting feature of latex allergy in 11 of the 35 patients. Using in vitro assays, we were able to detect latex-specific immunoglobulin E in the sera of all but two of our patients. Latex gloves and latex-containing intravenous sets were common triggers for reactions. When exposure to latex occurs systemically, as through an intravenous line, premedication with steroids and antihistamines may fail to protect against anaphylaxis. Conclusions. Our experience indicates that the incidence of latex hypersensitivity in children is increasing, that the circumstances (patient profile, hospital location, route of exposure) in which life-threatening reactions may occur are more broad than previously reported, and that a better understanding of both environmental sources of latex antigens and host responses to latex exposure are needed for improved prevention of serious reactions.

scholarly journals Diagnostic Value of Antigen-Specific Immunoglobulin E Immunoassays against Ara h 2 and Ara h 8 Peanut Components in Child Food Allergy

2016 ◽  
Vol 169 (4) ◽  
pp. 216-222 ◽  
Author(s):  
Jérémie Martinet ◽  
Laure Couderc ◽  
Florian Renosi ◽  
Victor Bobée ◽  
Christophe Marguet ◽  
...  
Keyword(s):  
Food Allergy ◽  
Immunoglobulin E ◽  
Diagnostic Value ◽  
Ara H 2 ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

Evaluation of a fully automated and completely quantitative allergen-specific IgE and total IgE detection assay

2021 ◽  
Author(s):  
Huimin Huang ◽  
Zhifeng Huang ◽  
Peiyan Zheng ◽  
Nili Wei ◽  
Xueqing Liang ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Detection System ◽  
Specific Ige ◽  
Quantitative Detection ◽  
Total Ige ◽  
Detection Assay ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

Based on the current urgent need for an in vitro quantitative detection system for allergens in most hospitals in China, we introduced a novel allergen-specific immunoglobulin E detection system that...

scholarly journals The Epidemiology of Food Allergy in the Global Context

2018 ◽  
Vol 15 (9) ◽  
pp. 2043 ◽  
Author(s):  
Wenyin Loh ◽  
Mimi Tang
Keyword(s):  
Developing Countries ◽  
Food Allergy ◽  
Immunoglobulin E ◽  
Clinical History ◽  
Food Allergies ◽  
Western Countries ◽  
Specific Immunoglobulin E ◽  
Food Challenges ◽  
Surrogate Measures ◽  
Allergy Prevalence

There is a lack of high-quality evidence based on the gold standard of oral food challenges to determine food allergy prevalence. Nevertheless, studies using surrogate measures of food allergy, such as health service utilization and clinical history, together with allergen-specific immunoglobulin E (sIgE), provide compelling data that the prevalence of food allergy is increasing in both Western and developing countries. In Western countries, challenge-diagnosed food allergy has been reported to be as high as 10%, with the greatest prevalence noted among younger children. There is also growing evidence of increasing prevalence in developing countries, with rates of challenge-diagnosed food allergy in China and Africa reported to be similar to that in Western countries. An interesting observation is that children of East Asian or African descent born in a Western environment are at higher risk of food allergy compared to Caucasian children; this intriguing finding emphasizes the importance of genome-environment interactions and forecasts future increases in food allergy in Asia and Africa as economic growth continues in these regions. While cow’s milk and egg allergy are two of the most common food allergies in most countries, diverse patterns of food allergy can be observed in individual geographic regions determined by each country’s feeding patterns. More robust studies investigating food allergy prevalence, particularly in Asia and the developing world, are necessary to understand the extent of the food allergy problem and identify preventive strategies to cope with the potential increase in these regions.

Efficacy of hyposensitization in feline allergic diseases based upon results of in vitro testing for allergen-specific immunoglobulin E

1997 ◽  
Vol 33 (3) ◽  
pp. 282-288 ◽  
Author(s):  
RE Halliwell
Keyword(s):  
Allergic Disease ◽  
Immunoglobulin E ◽  
Clinical Signs ◽  
Allergic Diseases ◽  
Dietary Change ◽  
In Vitro Tests ◽  
Similar Response ◽  
Specific Immunoglobulin E ◽  
Specific Immunoglobulin

A survey was undertaken to evaluate the responses to hyposensitization in cats for which a diagnosis of allergic disease was made by assessment of clinical signs and evaluation of allergen-specific immunoglobulin E (IgE) using the radioallergosorbent test (RAST). Eighty-one cases were available for analysis. In 39 cases, a dietary change resulted in some subjective improvement, which usually was minimal; however, some cases had significant improvement. In 75.3% of cases, an improvement of at least 50% was noted and ascribed to a combination of immunotherapy and dietary change, when implemented. The response of the 42 cases in which immunotherapy alone was used or where there was no response to dietary change was broadly similar. Response of the specific dermatological conditions ranged from 93.6% in the case of linear granuloma to 60% in cases where self-induced hair loss was evident. Response also was good in cases of presumed allergic asthma (86.1% improvement) and suspected allergic otitis externa (62.6% improvement). In nine patients, the hyposensitization was discontinued due to lack of response. The results suggest that confirmation of a suspected diagnosis of allergic disease in cats by means of in vitro tests and subsequent therapy with hyposensitization should be a major consideration in feline practice.

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