Performance of Four Common Tissue Preservatives for Immunohistochemical Testing of Cytology Specimens; Preliminary Findings from an Immunohistochemical Stains Validation Project

Abstract Sarcomatoid carcinoma of the small bowel is rare; to our knowledge, 19 cases have been reported to date in the English literature under several names. We report an additional case occurring in the jejunum of a 55-year-old man. The tumor was a polypoid 7.5-cm mass, which infiltrated the full thickness of the intestinal wall and the serosa of an adhesed loop of small bowel. On microscopic examination, the neoplasm was composed of sheets of spindle cells; focally, an anaplastic component was present, including tumor giant cells with bizarre nuclei. On immunohistochemical stains, tumor cells were positive for cytokeratin 7, cytokeratin AE1/AE3, vimentin, and focally, epithelial membrane antigen. No staining for cytokeratin 20 was found. Sarcomatoid carcinoma must be kept in mind in the differential diagnosis of malignant spindle cell tumors of the small bowel. As consensus regarding the terminology of these rare tumors is being reached, immunohistochemical stains are essential for accurate diagnosis.

Download Full-text

Urothelial and Prostate Carcinoma Metastasizing to the Same Lymph Node

Archives of Pathology & Laboratory Medicine ◽

10.5858/2001-125-1354-uapcmt ◽

2001 ◽

Vol 125 (10) ◽

pp. 1354-1357 ◽

Cited By ~ 3

Author(s):

Kerith Overstreet ◽

Parviz Haghighi

Keyword(s):

Lymph Node ◽

Urothelial Carcinoma ◽

Internal Control ◽

Specific Antigen ◽

Collision Tumor ◽

Second Primary ◽

Gleason Grade ◽

Primary Tumors ◽

Collision Tumors ◽

Immunohistochemical Stains

Abstract We report herein a case of a collision tumor composed of high-grade urothelial carcinoma and a Gleason grade 3+4 prostate adenocarcinoma metastasizing to the same lymph node. After the patient underwent cystoprostatectomy for known urothelial carcinoma, he was incidentally discovered to have a second primary prostate tumor. Lymph node examination revealed that one node appeared to have metastatic foci from both primary tumors. The presence of 2 tumor types colliding in the same lymph node was confirmed using immunohistochemical stains, including monoclonal carcinoembryonic antigen, prostate-specific antigen, prostatic acid phosphatase, cytokeratins 7 and 20, and CD57. We also stained both primary tumors with the same panel as an internal control. Although 2 similar collision tumors have been reported in the literature in the past, neither used a battery of immunohistochemical stains to definitively distinguish one tumor from the other. Herein, we review the literature on urothelial and prostate collision tumors and some of the special stains used to distinguish them.

Download Full-text

Alveolar Soft Part Sarcoma

Archives of Pathology & Laboratory Medicine ◽

10.5858/2007-131-488-asps ◽

2007 ◽

Vol 131 (3) ◽

pp. 488-491

Author(s):

Neda Zarrin-Khameh ◽

Kim S. Kaye

Keyword(s):

Poor Prognosis ◽

Soft Part ◽

Periodic Acid ◽

Alveolar Soft Part Sarcoma ◽

Diagnostic Feature ◽

Periodic Acid Schiff ◽

Radiographic Features ◽

Rare Neoplasm ◽

Immunohistochemical Stains

Abstract This article provides an overview of the pathology of alveolar soft part sarcoma, focused on its morphology, special stains useful in diagnosis, and the clinical and radiographic features of the disease. Alveolar soft part sarcoma is a rare neoplasm of unknown histogenesis with poor prognosis. Although there are several immunohistochemical stains available to help reach the diagnosis, the morphology of the tumor should be considered the main diagnostic feature. The periodic acid–Schiff stain is the best single stain that supports the diagnosis.

Download Full-text

Conjunctival myxoid stromal tumour: a distinctive clinicopathological and immunohistochemical study

British Journal of Ophthalmology ◽

10.1136/bjophthalmol-2018-312747 ◽

2018 ◽

Vol 103 (9) ◽

pp. 1259-1265 ◽

Cited By ~ 4

Author(s):

Xiao-Yi Qin ◽

Zhe-Hao Jin ◽

You-Pei Wang ◽

Zong-Duan Zhang

Keyword(s):

Smooth Muscle Actin ◽

Periodic Acid ◽

Clinical Findings ◽

Stromal Tumour ◽

Bulbar Conjunctiva ◽

Low Grade ◽

Ki 67 ◽

Periodic Acid Schiff ◽

Multiple Spindle ◽

Immunohistochemical Stains

Background/aimsTo describe the clinicopathological and immunohistochemical characteristics of 10 patients representing a new entity of benign conjunctival myxoid stromal tumours.MethodsRetrospective review of clinical findings, histopathological and immunohistochemical studies identified 10 cases of low-grade conjunctival myxoid stromal tumours. Specimens were routinely processed and stained with H&E. Immunohistochemical stains for CD34, CD68, vimentin, S100, smooth muscle actin (SMA), myosin, desmin, actin, Bcl-2 and Ki-67 were performed. Specific stains for Alcian-blue periodic acid-Schiff (AB-PAS) and aldehyde fuchsin stains were also performed.ResultsTen patients with an average age of 45.6±11.1 years had a tender white or faint yellow to red mass on the bulbar conjunctiva. All the lesions were completely removed, and none of the patients relapsed. Histologically, all neoplasms consisted of spindle-shaped cells that showed signs of pseudonuclear inclusions, multinuclear cells and had no atypia. The stroma consisted of a large amount of mucus and was infiltrated with delicate to ropey collagens, a few mast cells and new vessels. Immunohistochemical stains were positive for CD34, vimentin and Bcl-2; partial positive for CD68; very low for Ki-67; and negative for S100, SMA, myosin, desmin and actin. AB-PAS suggested that the stroma was mucinous.ConclusionsThese rare benign mesenchymal conjunctival tumours are mostly unilateral and occur in the bulbar conjunctiva. Complete resection is the radical treatment. These lesions are characterised by multiple spindle cells, a large amount of mucus, and sharing similar basic histopathological features with conjunctival myxoma and conjunctival stromal tumour. We suggest naming these lesions ‘conjunctival myxoid stromal tumours’.

Download Full-text