Competitive allele-specific short oligonucleotide hybridization (CASSOH) with enzyme-linked immunosorbent assay (ELISA) for the detection of pharmacogenetic single nucleotide polymorphisms (SNPs)

2006 ◽  
Vol 67 (2-3) ◽  
pp. 87-94
Author(s):  
Masahiro Hiratsuka ◽  
Aiko Ebisawa ◽  
Kanako Sakuyama ◽  
Yoichi Matsubara ◽  
Shigeo Kure ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Short Oligonucleotide ◽  
Oligonucleotide Hybridization ◽  
Allele Specific

Fabrication and Application of Single Nucleotide Polymorphisms Library on Magnetic Nanoparticles Using Adaptor PCR

2008 ◽  
Vol 8 (1) ◽  
pp. 405-409 ◽  
Author(s):  
Hongna Liu ◽  
Song Li ◽  
Meiju Ji ◽  
Libo Nie ◽  
Jianrong Chen ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Single Nucleotide Polymorphisms ◽  
Reliable Method ◽  
Nucleotide Polymorphisms ◽  
Probe Signal ◽  
Single Nucleotide ◽  
Novel Approach ◽  
Mismatch Probe ◽  
Allele Specific ◽  
Agt Gene

We have developed a novel approach to fabricate single nucleotide polymorphisms (SNPs) library on magnetic nanoparticles (MNPs) based on adaptor PCR. Each SNP locus in the library was interrogated by hybridization with a pair of allele specific dual-color fluorescence (Cy3, Cy5) probes to determine SNP. Two SNPs loci (M235T and A-6G) associated with essential hypertension in the angiotensinogen (AGT) gene were detected by this method and their fluorescent signals were quantified. The fluorescent ratios (match probe: mismatch probe signal) of homozygous genotypes were over 3.0, whereas heterozygous genotypes had ratios near to 1.0. Without any complex multiplex PCR procedure, it is a simple, efficient and reliable method for the multiplex SNPs detection using limited amount of DNA samples from individuals.

Sign in / Sign up

Export Citation Format

Share Document