Evaluating new bio-hydrogen producers: Clostridium perfringens strain JJC, Clostridium bifermentans strain WYM and Clostridium sp. strain Ade.TY

ABSTRACT The gene encoding Clostridium sordellii phospholipase C (Csp) was cloned and expressed as a histidine-tagged (His-tag) protein, and the protein was purified to compare its enzymatic and biological activities with those of Clostridium perfringens phospholipase C (Cpa) and Clostridium bifermentans phospholipase C (Cbp). Csp was found to consist of 371 amino acid residues in the mature form and to be more homologous to Cbp than to Cpa. The egg yolk phospholipid hydrolysis activity of the His-tag Csp was about one-third of that of His-tag Cpa, but the hemolytic activity was less than 1% of that of His-tag Cpa. His-tag Csp was nontoxic to mice. Immunization of mice with His-tag Cbp or His-tag Csp did not provide effective protection against the lethal activity of His-tag Cpa. These results indicate that Csp possesses similar molecular properties to Cbp and suggest that comparative analysis of toxic and nontoxic clostridial phospholipases is helpful for characterization of the toxic properties of clostridial phospholipases.

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Cloning and expression of the phospholipase C gene from Clostridium perfringens and Clostridium bifermentans.

Infection and Immunity ◽

10.1128/iai.57.2.468-476.1989 ◽

1989 ◽

Vol 57 (2) ◽

pp. 468-476 ◽

Cited By ~ 50

Author(s):

J Y Tso ◽

C Siebel

Keyword(s):

Phospholipase C ◽

Clostridium Perfringens ◽

Cloning And Expression ◽

Clostridium Bifermentans

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Die nekrotisierende Enteritis des Saugferkels durch Clostridium perfringens Typ C: I. Beobachtungen zu Klinik, Bekämpfung und Epidemiologie

Schweizer Archiv für Tierheilkunde ◽

10.1024/0036-7281.144.6.263 ◽

2002 ◽

Vol 144 (6) ◽

pp. 263-273 ◽

Cited By ~ 2

Author(s):

A. Luginbühl

Keyword(s):

Clostridium Perfringens

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Possible influence of herd health management and hygiene on the in-herd prevalence of Clostridium perfringens type C in pig breeding farms

Schweizer Archiv für Tierheilkunde ◽

10.1024/0036-7281/a000503 ◽

2013 ◽

Vol 155 (9) ◽

pp. 520-522 ◽

Cited By ~ 3

Author(s):

K. Schäfer ◽

W. Zimmermann ◽

H. Posthaus

Keyword(s):

Clostridium Perfringens ◽

Health Management ◽

Herd Health ◽

Herd Prevalence ◽

Type C ◽

Breeding Farms

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State of Alaska epidemiology bulletin: Clostridium perfringens

PsycEXTRA Dataset ◽

10.1037/e447162008-001 ◽

1985 ◽

Keyword(s):

Clostridium Perfringens

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Phospholipase C from Clostridium Perfringens Induces Human Platelet Aggregation in Plasma

Thrombosis and Haemostasis ◽

10.1055/s-0038-1642761 ◽

1988 ◽

Vol 59 (02) ◽

pp. 236-239 ◽

Cited By ~ 2

Author(s):

Giovanna Barzaghi ◽

Chiara Cerletti ◽

Giovanni de Gaetano

Keyword(s):

Platelet Aggregation ◽

Receptor Antagonist ◽

Phospholipase C ◽

Clostridium Perfringens ◽

Human Platelet ◽

Ex Vivo ◽

Platelet Rich Plasma ◽

Platelet Activating Factor ◽

Inhibitory Effect ◽

Thromboxane Receptor Antagonist

SummaryWe studied the aggregating effect of different concentrations of phospholipase C (PLC) (extracted from Clostridium perfringens) on human platelet-rich plasma (PRP). PRP was preincubated with PLC for 3 min at 37° C and the platelet aggregation was followed for 10 min. The threshold aggregating concentration (TAG) of PLC was 3-4 U/ml.We also studied the potentiation of PLC with other stimuli on platelet aggregation. Potentiating stimuli, such as arachidonic acid (AA), ADP. Platelet Activating Factor (PAF) and U-46619 (a stable analogue of cyclic endoperoxides) were all used at subthreshold concentrations. We also studied the possible inhibitory effect of aspirin, apyrase, TMQ, a prostaglandin endoper- oxide/thromboxane receptor antagonist and BN-52021, a PAF receptor antagonist. Only aspirin and apyrase were able to reduce aggregation induced by PLC alone and PLC + AA and PLC + ADP respectively. TMQ and BN-52021 were inactive. In ex vivo experiments oral aspirin (500 mg) partially inhibited platelet aggregation induced by PLC alone, PLC + AA and PLC + ADP 2 and 24 h after administration. Aspirin 20 mg for 7 days also reduced aggregation induced by PLC + AA.

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