Fast enantiomeric separation of amino acids using liquid chromatography/mass spectrometry on a chiral crown ether stationary phase

2020 ◽  
Vol 130 (4) ◽  
pp. 437-442 ◽  
Author(s):  
Kohei Yoshikawa ◽  
Masahiro Furuno ◽  
Nobuo Tanaka ◽  
Eiichiro Fukusaki
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Liquid Chromatography ◽  
Crown Ether ◽  
Stationary Phase ◽  
Enantiomeric Separation ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chiral Crown Ether ◽  
Chromatography Mass Spectrometry

scholarly journals Cimodo virus belongs to a novel lineage of reoviruses isolated from African mosquitoes

2014 ◽  
Vol 95 (4) ◽  
pp. 905-909 ◽  
Author(s):  
Kyra Hermanns ◽  
Florian Zirkel ◽  
Andreas Kurth ◽  
Christian Drosten ◽  
Sandra Junglen
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Liquid Chromatography ◽  
Cote D'ivoire ◽  
Côte D’Ivoire ◽  
Viral Proteins ◽  
Liquid Chromatography Mass Spectrometry ◽  
Entire Genome ◽  
Cote D’Ivoire ◽  
Chromatography Mass Spectrometry

A novel reovirus, designated Cimodo virus (CMDV), was isolated from mosquitoes collected in a rainforest region in Côte d’Ivoire. The entire genome comprised 24 835 bp divided into 12 segments ranging from 585 to 4080 bp. The icosahedral non-enveloped virions were 80 nm in diameter. Eight major viral proteins of about 150, 135, 120, 80, 66, 59, 42 and 30 kDa were identified and seven proteins were mapped to the corresponding genome segments by liquid chromatography mass spectrometry. Predicted protein genes diverged by >77 % encoded amino acids from their closest reovirus relatives. The deep phylogenetic branching suggests that CMDV defines an as-yet-unidentified genus within the subfamily Spinareovirinae.

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