An effective cold inducible expression system developed in Pseudoalteromonas haloplanktis TAC125

2007 ◽  
Vol 127 (2) ◽  
pp. 199-210 ◽  
Author(s):  
Rosanna Papa ◽  
Valentina Rippa ◽  
Giovanni Sannia ◽  
Gennaro Marino ◽  
Angela Duilio
Keyword(s):  
Expression System ◽  
Inducible Expression ◽  
Pseudoalteromonas Haloplanktis ◽  
Inducible Expression System ◽  
Pseudoalteromonas Haloplanktis Tac125

scholarly journals An Inducible Expression System to Measure Rhodopsin Transport in Transgenic Xenopus Rod Outer Segments

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e82629 ◽  
Author(s):  
Xinming Zhuo ◽  
Mohammad Haeri ◽  
Eduardo Solessio ◽  
Barry E. Knox
Keyword(s):  
Expression System ◽  
Inducible Expression ◽  
Rod Outer Segments ◽  
Inducible Expression System ◽  
Outer Segments

scholarly journals A new inducible expression system in a transformed green alga, Chlorella vulgaris

2011 ◽  
Vol 10 (4) ◽  
pp. 3427-3434 ◽  
Author(s):  
Y.F. Niu ◽  
M.H. Zhang ◽  
W.H. Xie ◽  
J.N. Li ◽  
Y.F. Gao ◽  
...  
Keyword(s):  
Green Alga ◽  
Chlorella Vulgaris ◽  
Expression System ◽  
Inducible Expression ◽  
Inducible Expression System ◽  
Green Alga Chlorella

Development of an anhydrotetracycline-inducible expression system for expression of a neopullulanase in B. subtilis

Plasmid ◽  
2015 ◽  
Vol 82 ◽  
pp. 35-42 ◽  
Author(s):  
Kambiz Morabbi Heravi ◽  
Hildegard Watzlawick ◽  
Josef Altenbuchner
Keyword(s):  
Expression System ◽  
Inducible Expression ◽  
Inducible Expression System

scholarly journals Construction and Characterization of a Gradually Inducible Expression Vector for Halobacterium salinarum, Based on thekdpPromoter

2012 ◽  
Vol 78 (7) ◽  
pp. 2100-2105 ◽  
Author(s):  
Dorthe Kixmüller ◽  
Jörg-Christian Greie
Keyword(s):  
Gene Expression ◽  
Expression Vector ◽  
Expression System ◽  
Expression Patterns ◽  
Inducible Expression ◽  
Halobacterium Salinarum ◽  
Expression Vectors ◽  
Content Type ◽  
Inducible Expression System

ABSTRACTGradually inducible expression vectors which are governed by variations of growth conditions are powerful tools for gene expression of conditionally lethal mutants. Furthermore, controlled expression allows monitoring of overproduction of proteins at various stages in their expressing hosts. ForHalobacterium salinarum, which is often used as a paradigm for halophilic archaea, such an inducible expression system is not available to date. Here we show that thekdppromoter (Pkdp), which facilitates gene expression upon K+limitation, can be used to establish such a system for molecular applications. Pkdpfeatures a rather high expression rate, with an approximately 50-fold increase that can be easily varied by K+concentrations in the growth medium. Besides the construction of an expression vector, our work describes the characterization of expression patterns and, thus, offers a gradually inducible expression system to the scientific community.

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