Development of an anhydrotetracycline-inducible expression system for expression of a neopullulanase in B. subtilis

Plasmid ◽  
2015 ◽  
Vol 82 ◽  
pp. 35-42 ◽  
Author(s):  
Kambiz Morabbi Heravi ◽  
Hildegard Watzlawick ◽  
Josef Altenbuchner
PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e82629 ◽  
Author(s):  
Xinming Zhuo ◽  
Mohammad Haeri ◽  
Eduardo Solessio ◽  
Barry E. Knox

2011 ◽  
Vol 10 (4) ◽  
pp. 3427-3434 ◽  
Author(s):  
Y.F. Niu ◽  
M.H. Zhang ◽  
W.H. Xie ◽  
J.N. Li ◽  
Y.F. Gao ◽  
...  

2012 ◽  
Vol 78 (7) ◽  
pp. 2100-2105 ◽  
Author(s):  
Dorthe Kixmüller ◽  
Jörg-Christian Greie

ABSTRACTGradually inducible expression vectors which are governed by variations of growth conditions are powerful tools for gene expression of conditionally lethal mutants. Furthermore, controlled expression allows monitoring of overproduction of proteins at various stages in their expressing hosts. ForHalobacterium salinarum, which is often used as a paradigm for halophilic archaea, such an inducible expression system is not available to date. Here we show that thekdppromoter (Pkdp), which facilitates gene expression upon K+limitation, can be used to establish such a system for molecular applications. Pkdpfeatures a rather high expression rate, with an approximately 50-fold increase that can be easily varied by K+concentrations in the growth medium. Besides the construction of an expression vector, our work describes the characterization of expression patterns and, thus, offers a gradually inducible expression system to the scientific community.


2007 ◽  
Vol 127 (2) ◽  
pp. 199-210 ◽  
Author(s):  
Rosanna Papa ◽  
Valentina Rippa ◽  
Giovanni Sannia ◽  
Gennaro Marino ◽  
Angela Duilio

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