Engineering a Native Inducible Expression System in Shewanella oneidensis to Control Extracellular Electron Transfer

2017 ◽  
Vol 6 (9) ◽  
pp. 1627-1634 ◽  
Author(s):  
Elizabeth A. West ◽  
Abhiney Jain ◽  
Jeffrey A. Gralnick
Keyword(s):  
Electron Transfer ◽  
Expression System ◽  
Shewanella Oneidensis ◽  
Inducible Expression ◽  
Extracellular Electron Transfer ◽  
Inducible Expression System

scholarly journals Developing a population-state decision system for intelligently reprogramming extracellular electron transfer in Shewanella oneidensis

2020 ◽  
Vol 117 (37) ◽  
pp. 23001-23010 ◽  
Author(s):  
Feng-He Li ◽  
Qiang Tang ◽  
Yang-Yang Fan ◽  
Yang Li ◽  
Jie Li ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Bacterial Growth ◽  
Metabolic Flux ◽  
Expression System ◽  
Shewanella Oneidensis ◽  
Cellular Growth ◽  
Extracellular Electron Transfer ◽  
Regulation System ◽  
Growth State ◽  
Pollutant Reduction

The unique extracellular electron transfer (EET) ability has positioned electroactive bacteria (EAB) as a major class of cellular chassis for genetic engineering aimed at favorable environmental, energy, and geoscience applications. However, previous efforts to genetically enhance EET ability have often impaired the basal metabolism and cellular growth due to the competition for the limited cellular resource. Here, we design a quorum sensing-based population-state decision (PSD) system for intelligently reprogramming the EET regulation system, which allows the rebalanced allocation of the cellular resource upon the bacterial growth state. We demonstrate that the electron output from Shewanella oneidensis MR-1 could be greatly enhanced by the PSD system via shifting the dominant metabolic flux from initial bacterial growth to subsequent EET enhancement (i.e., after reaching a certain population-state threshold). The strain engineered with this system achieved up to 4.8-fold EET enhancement and exhibited a substantially improved pollutant reduction ability, increasing the reduction efficiencies of methyl orange and hexavalent chromium by 18.8- and 5.5-fold, respectively. Moreover, the PSD system outcompeted the constant expression system in managing EET enhancement, resulting in considerably enhanced electron output and pollutant bioreduction capability. The PSD system provides a powerful tool for intelligently managing extracellular electron transfer and may inspire the development of new-generation smart bioelectrical devices for various applications.

scholarly journals An Inducible Expression System to Measure Rhodopsin Transport in Transgenic Xenopus Rod Outer Segments

PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e82629 ◽  
Author(s):  
Xinming Zhuo ◽  
Mohammad Haeri ◽  
Eduardo Solessio ◽  
Barry E. Knox
Keyword(s):  
Expression System ◽  
Inducible Expression ◽  
Rod Outer Segments ◽  
Inducible Expression System ◽  
Outer Segments

Impact of Graphitic Structures in Pyrogenic Carbon on Microbial Reduction of Ferrihydrite

2021 ◽  
Author(s):  
wentao yu ◽  
baoliang chen
Keyword(s):  
Electron Transfer ◽  
Pyrolysis Temperature ◽  
Shewanella Oneidensis ◽  
Exchange Capacity ◽  
Microbial Reduction ◽  
Systematic Evaluation ◽  
Extracellular Electron Transfer ◽  
Pyrogenic Carbon ◽  
The Impact

<p>Pyrogenic carbon plays important roles in microbial reduction of ferrihydrite by shuttling electrons in the extracellular electron transfer (EET) processes. Despite its importance, a full assessment on the impact of graphitic structures in pyrogenic carbon on microbial reduction of ferrihydrite has not been conducted. This study is a systematic evaluation of microbial ferrihydrite reduction by Shewanella oneidensis MR-1 in the presence of pyrogenic carbon with various graphitization extents. The results showed that the rates and extents of microbial ferrihydrite reduction were significantly enhanced in the presence of pyrogenic carbon, and increased with increasing pyrolysis temperature. Combined spectroscopic and electrochemical analyses suggested that the rate of microbial ferrihydrite reduction were dependent on the electrical conductivity of pyrogenic carbon (i.e., graphitization extent), rather than the electron exchange capacity. The key role of graphitic structures in pyrogenic carbon in mediating EET was further evidenced by larger microbial electrolysis current with pyrogenic carbon prepared at higher pyrolysis temperatures. This study provides new insights into the electron transfer in the pyrogenic carbon-mediated microbial reduction of ferrihydrite.</p>

scholarly journals A new inducible expression system in a transformed green alga, Chlorella vulgaris

2011 ◽  
Vol 10 (4) ◽  
pp. 3427-3434 ◽  
Author(s):  
Y.F. Niu ◽  
M.H. Zhang ◽  
W.H. Xie ◽  
J.N. Li ◽  
Y.F. Gao ◽  
...  
Keyword(s):  
Green Alga ◽  
Chlorella Vulgaris ◽  
Expression System ◽  
Inducible Expression ◽  
Inducible Expression System ◽  
Green Alga Chlorella

Identifying the potential extracellular electron transfer pathways from a c-type cytochrome network

2014 ◽  
Vol 10 (12) ◽  
pp. 3138-3146 ◽  
Author(s):  
De-Wu Ding ◽  
Jun Xu ◽  
Ling Li ◽  
Jian-Ming Xie ◽  
Xiao Sun
Keyword(s):  
Electron Transfer ◽  
Shewanella Oneidensis ◽  
Extracellular Electron Transfer ◽  
Genome Wide ◽  
A Genome ◽  
Electron Transfer Pathways

A genome-wide c-type cytochrome network was constructed to explore the extracellular electron transfer pathways in Shewanella oneidensis MR-1.

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