High lipid accumulation in Yarrowia lipolytica cultivated under double limitation of nitrogen and magnesium

2016 ◽  
Vol 234 ◽  
pp. 116-126 ◽  
Author(s):  
S. Bellou ◽  
I.-E. Triantaphyllidou ◽  
P. Mizerakis ◽  
G. Aggelis
Keyword(s):  
Yarrowia Lipolytica ◽  
Lipid Accumulation ◽  
High Lipid

scholarly journals Snf1 Is a Regulator of Lipid Accumulation in Yarrowia lipolytica

2013 ◽  
Vol 79 (23) ◽  
pp. 7360-7370 ◽  
Author(s):  
John Seip ◽  
Raymond Jackson ◽  
Hongxian He ◽  
Quinn Zhu ◽  
Seung-Pyo Hong
Keyword(s):  
Yarrowia Lipolytica ◽  
Lipid Accumulation ◽  
Oleaginous Yeast ◽  
De Novo ◽  
Lipid Synthesis ◽  
Omega 3 ◽  
Wild Type ◽  
Content Type ◽  
Reverse Transcription Pcr ◽  
Dry Cell Weight

ABSTRACTIn the oleaginous yeastYarrowia lipolytica,de novolipid synthesis and accumulation are induced under conditions of nitrogen limitation (or a high carbon-to-nitrogen ratio). The regulatory pathway responsible for this induction has not been identified. Here we report that the SNF1 pathway plays a key role in the transition from the growth phase to the oleaginous phase inY. lipolytica. Strains with aY. lipolyticasnf1(Ylsnf1) deletion accumulated fatty acids constitutively at levels up to 2.6-fold higher than those of the wild type. When introduced into aY. lipolyticastrain engineered to produce omega-3 eicosapentaenoic acid (EPA),Ylsnf1deletion led to a 52% increase in EPA titers (7.6% of dry cell weight) over the control. Other components of theY. lipolyticaSNF1 pathway were also identified, and their function in limiting fatty acid accumulation is suggested by gene deletion analyses. Deletion of the gene encoding YlSnf4, YlGal83, or YlSak1 significantly increased lipid accumulation in both growth and oleaginous phases compared to the wild type. Furthermore, microarray and quantitative reverse transcription-PCR (qRT-PCR) analyses of theYlsnf1mutant identified significantly differentially expressed genes duringde novolipid synthesis and accumulation inY. lipolytica. Gene ontology analysis found that these genes were highly enriched with genes involved in lipid metabolism. This work presents a new role for Snf1/AMP-activated protein kinase (AMPK) pathways in lipid accumulation in this oleaginous yeast.

scholarly journals Initial study of lipid accumulation in green algal Haematococcus pluvialis Flotow cultured in liquid Bold’s Basal medium aerated

2019 ◽  
Vol 3 (3) ◽  
pp. 144-149
Author(s):  
Nguyen Tran Dong Phuong ◽  
Le Huyen Ai Thuy ◽  
Bui Trang Viet
Keyword(s):  
Lipid Accumulation ◽  
Fatty Acid Biosynthesis ◽  
Haematococcus Pluvialis ◽  
Acyl Carrier Protein ◽  
Nile Red ◽  
Basal Medium ◽  
Initial Study ◽  
Rt Pcr ◽  
Green Algal ◽  
High Lipid

The fresh green algal Haematococcus pluvialis Flotow was proved to be the starting material for the production of biofuel, high lipid content along with astaxanthin, a high value colorant. In this study, lipid accumulation in H. pluvialis cultured in liquid Bold’s Basal medium aerated was investigated for a period of 12 weeks. Lipid accumulation was evaluated through the expression of two genes: BC (biotin carboxylase, initial gene) and FATA (acyl-acyl carrier protein thioesterase, end gene) in the process of fatty acid biosynthesis with Real-time RT-PCR, lipid determination by Nile Red and biodiesel quantifying by transesterification. The results showed that the expression of two BC and FATA genes was recorded at all weeks of culture. However, the expression of BC and FATA genes increased gradually from the week 9 (1.3, 4.1, respectively) to week 11 (1.7, 30.9, respectively). Meanwhile, yellow fluorescence in the microalgal cells showed that lipid appeared from week 6 to week 12. The obtained biodiesel increased slowly from week 8 (0.036 mg/mL) to week 12 (0.041 mg/mL). At week 11, the expression values of both BC gene (1.7) and FATA gene (30.9) were maximized, leading to the highest biodiesel content at the week 12.

scholarly journals Control of Lipid Accumulation in the Yeast Yarrowia lipolytica

2008 ◽  
Vol 74 (24) ◽  
pp. 7779-7789 ◽  
Author(s):  
Athanasios Beopoulos ◽  
Zuzana Mrozova ◽  
France Thevenieau ◽  
Marie-Thérèse Le Dall ◽  
Ivan Hapala ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Lipid Composition ◽  
Lipid Accumulation ◽  
Wild Type Strain ◽  
Lipid Synthesis ◽  
Genomic Comparison ◽  
Fourfold Increase ◽  
Glycerol 3 Phosphate Dehydrogenase ◽  
Acyl Coenzyme A ◽  
Yeast Yarrowia Lipolytica

ABSTRACT A genomic comparison of Yarrowia lipolytica and Saccharomyces cerevisiae indicates that the metabolism of Y. lipolytica is oriented toward the glycerol pathway. To redirect carbon flux toward lipid synthesis, the GUT2 gene, which codes for the glycerol-3-phosphate dehydrogenase isomer, was deleted in Y. lipolytica in this study. This Δgut2 mutant strain demonstrated a threefold increase in lipid accumulation compared to the wild-type strain. However, mobilization of lipid reserves occurred after the exit from the exponential phase due to β-oxidation. Y. lipolytica contains six acyl-coenzyme A oxidases (Aox), encoded by the POX1 to POX6 genes, that catalyze the limiting step of peroxisomal β-oxidation. Additional deletion of the POX1 to POX6 genes in the Δgut2 strain led to a fourfold increase in lipid content. The lipid composition of all of the strains tested demonstrated high proportions of FFA. The size and number of the lipid bodies in these strains were shown to be dependent on the lipid composition and accumulation ratio.

scholarly journals A Mathematical Model of Neutral Lipid Content in terms of Initial Nitrogen Concentration and Validation in Coelastrum sp. HA-1 and Application in Chlorella sorokiniana

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Zhenhua Yang ◽  
Yue Zhao ◽  
Zhiyong Liu ◽  
Chenfeng Liu ◽  
Zhipeng Hu ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Lipid Content ◽  
Lipid Accumulation ◽  
Nitrogen Concentration ◽  
Lipid Production ◽  
Lipid Synthesis ◽  
Quantitative Relationship ◽  
Chlorella Sorokiniana ◽  
High Lipid ◽  
Nitrogen Concentrations

Microalgae are considered to be a potential major biomass feedstock for biofuel due to their high lipid content. However, no correlation equations as a function of initial nitrogen concentration for lipid accumulation have been developed for simplicity to predict lipid production and optimize the lipid production process. In this study, a lipid accumulation model was developed with simple parameters based on the assumption protein synthesis shift to lipid synthesis by a linear function of nitrogen quota. The model predictions fitted well for the growth, lipid content, and nitrogen consumption of Coelastrum sp. HA-1 under various initial nitrogen concentrations. Then the model was applied successfully in Chlorella sorokiniana to predict the lipid content with different light intensities. The quantitative relationship between initial nitrogen concentrations and the final lipid content with sensitivity analysis of the model were also discussed. Based on the model results, the conversion efficiency from protein synthesis to lipid synthesis is higher and higher in microalgae metabolism process as nitrogen decreases; however, the carbohydrate composition content remains basically unchanged neither in HA-1 nor in C. sorokiniana.

scholarly journals Molecular Mechanism of Citrate Efflux by the Mitochondrial Citrate Transporter CT in Filamentous Fungus Mucor circinelloides WJ11

2021 ◽  
Vol 12 ◽  
Author(s):  
Wu Yang ◽  
Shiqi Dong ◽  
Junhuan Yang ◽  
Hassan Mohamed ◽  
Aabid Manzoor Shah ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Filamentous Fungus ◽  
Lipid Accumulation ◽  
High Efficiency ◽  
Mucor Circinelloides ◽  
Transport Activity ◽  
Citrate Transport ◽  
Citrate Transporter ◽  
High Lipid ◽  
Citrate Efflux

The mitochondrial citrate transporter (MCT) plays an important role in citrate efflux from the mitochondria in eukaryotes, and hence provides a direct correlation between carbohydrate metabolism and lipid synthesis. Our previous studies on transporters confirmed the presence of two MCTs (TCT and CT) in oleaginous Mucor circinelloides WJ11 associated with high lipid accumulation. However, the molecular mechanism of citrate efflux from the mitochondria by MCT in M. circinelloides is still unclear. To study the citrate transport mechanism of CT, the citrate transporter gene was expressed in Escherichia coli, and its product was purified. The citrate transport activity of the protein was studied in CT reconstituted liposomes. Our results showed high efficiency of CT for [14C] citrate/citrate exchange with Km 0.01 mM at 25°C. Besides citrate, other molecules such as oxaloacetate, malate, fumarate, succinate aconitate, oxoadipate, isocitrate, and glutamate also promote citrate transport. In addition, the ct overexpression and knockout plasmids were constructed and transferred into M. circinelloides WJ11, and the mitochondria were isolated, and the transport activity was studied. Our findings showed that in the presence of 10 mM malate, the mitochondria of ct-overexpressing transformant showed 51% increase in the efflux rate of [14C] citrate, whereas the mitochondria of the ct-knockout transformant showed 18% decrease in citrate efflux compared to the mitochondria of wild-type WJ11. This study provided the first mechanistic evidence of citrate efflux from the mitochondria by citrate transporter in oleaginous filamentous fungus M. circinelloides, which is associated with high lipid accumulation.

Laboratory evolution strategies for improving lipid accumulation in Yarrowia lipolytica

2019 ◽  
Vol 103 (20) ◽  
pp. 8585-8596 ◽  
Author(s):  
Alexandra Daskalaki ◽  
Nikolitsa Perdikouli ◽  
Dimitra Aggeli ◽  
George Aggelis
Keyword(s):  
Yarrowia Lipolytica ◽  
Lipid Accumulation ◽  
Evolution Strategies ◽  
Laboratory Evolution

Co-expression of glucose-6-phosphate dehydrogenase and acyl-CoA binding protein enhances lipid accumulation in the yeast Yarrowia lipolytica

2017 ◽  
Vol 39 ◽  
pp. 18-21 ◽  
Author(s):  
Evgeniya Y. Yuzbasheva ◽  
Elizaveta B. Mostova ◽  
Natalia I. Andreeva ◽  
Tigran V. Yuzbashev ◽  
Ivan A. Laptev ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Lipid Accumulation ◽  
Binding Protein ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Yeast Yarrowia Lipolytica
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