scholarly journals A liquid/liquid two phase system as an economic alternative for the large-scale expansion of bone marrow-derived human mesenchymal stem/stromal cells (hMSCs)

2017 ◽  
Vol 256 ◽  
pp. S43
Author(s):  
Mariana Petronela Hanga ◽  
Alvin W. Nienow ◽  
Karen Coopman ◽  
Christopher J. Hewitt
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Large Scale ◽  
Phase System ◽  
Two Phase ◽  
Scale Expansion

Large-scale expansion of pre-isolated bone marrow mesenchymal stromal cells in serum-free conditions

2013 ◽  
Vol 10 (2) ◽  
pp. 108-119 ◽  
Author(s):  
Sanjay Gottipamula ◽  
Manjunatha S. Muttigi ◽  
S. Chaansa ◽  
K. M. Ashwin ◽  
Nancy Priya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Large Scale ◽  
Serum Free ◽  
Scale Expansion ◽  
Serum Free Conditions

scholarly journals A Method for Measurement of Drug Sensitivity of Myeloma Cells Co-Cultured with Bone Marrow Stromal Cells

2013 ◽  
Vol 18 (6) ◽  
pp. 637-646 ◽  
Author(s):  
Kristine Misund ◽  
Katarzyna A. Baranowska ◽  
Toril Holien ◽  
Christoph Rampa ◽  
Dionne C. G. Klein ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Survival ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Large Scale ◽  
Stromal Cell ◽  
Marrow Stromal Cells ◽  
Cell Nuclei ◽  
Myeloma Cells

The tumor microenvironment can profoundly affect tumor cell survival as well as alter antitumor drug activity. However, conventional anticancer drug screening typically is performed in the absence of stromal cells. Here, we analyzed survival of myeloma cells co-cultured with bone marrow stromal cells (BMSC) using an automated fluorescence microscope platform, ScanR. By staining the cell nuclei with DRAQ5, we could distinguish between BMSC and myeloma cells, based on their staining intensity and nuclear shape. Using the apoptotic marker YO-PRO-1, the effects of drug treatment on the viability of the myeloma cells in the presence of stromal cells could be measured. The method does not require cell staining before incubation with drugs, and less than 5000 cells are required per condition. The method can be used for large-scale screening of anticancer drugs on primary myeloma cells. This study shows the importance of stromal cell support for primary myeloma cell survival in vitro, as half of the cell samples had a marked increase in their viability when cultured in the presence of BMSC. Stromal cell–induced protection against common myeloma drugs is also observed with this method.

scholarly journals The effect of culture media on large-scale expansion and characteristic of adipose tissue-derived mesenchymal stromal cells

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Justyna Czapla ◽  
Sybilla Matuszczak ◽  
Klaudia Kulik ◽  
Ewa Wiśniewska ◽  
Ewelina Pilny ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Large Scale ◽  
Culture Media ◽  
Scale Expansion

Rapid Large-Scale Expansion of Functional Mesenchymal Stem Cells from Unmanipulated Bone Marrow Without Animal Serum

2008 ◽  
Vol 14 (3) ◽  
pp. 185-196 ◽  
Author(s):  
Katharina Schallmoser ◽  
Eva Rohde ◽  
Andreas Reinisch ◽  
Christina Bartmann ◽  
Daniela Thaler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Large Scale ◽  
Scale Expansion

scholarly journals Study on optimum technological conditions for producing androstenedione by microbial method

2019 ◽  
Vol 136 ◽  
pp. 06025
Author(s):  
Haishan SHI ◽  
Ying YANG ◽  
Weihua LI ◽  
Hui ZHANG ◽  
Xiaolei CHENG
Keyword(s):  
Substrate Concentration ◽  
Large Scale ◽  
Supply And Demand ◽  
Process Conditions ◽  
Phase System ◽  
Scale Production ◽  
Liquid Loading ◽  
Two Phase ◽  
Initial Ph ◽  
Promising Development

As an indispensable intermediate, androstenedione is widely used in drug manufacturing, especially steroidal drugs. However, the chemical manufacturing process of androstenedione is generally complicated and difficult, and it will cause serious environmental pollution in the production process. The biological method for the production of androstenedione has a very promising development prospect, because it is more economical and environmentally friendly than chemical methods. In order to better produce androstenedione on a large scale, the imbalance between supply and demand can be solved. In this study, the biaqueous phase system was used to increase the substrate concentration, and the method of transforming plant sterol by mycobacterium was used to produce androstenedione. The optimal conditions for the production of androstenedione by microbial assay were determined by orthogonal test: the aqueous two-phase system was water/ sunflower oil, the temperature was 30 °C, the initial pH was 6.5, the substrate concentration was 0.4 g/L, the rotation speed was 250 rpm, and the inoculation was carried out. The amount was 14.83%, the organic ratio was 20.65%, and the liquid loading was 150/500 mL. The preliminary production of androstenedione by microbial method has found suitable process conditions and provided data and theoretical support for its large-scale production.

Isolation and large-scale expansion of bone marrow-derived mesenchymal stem cells with serum-free media under GMP-compliance

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S111 ◽  
Author(s):  
S.H. Mei ◽  
M. Salkhordeh ◽  
F. Xue ◽  
J. Zhang ◽  
I. Watpool ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Large Scale ◽  
Serum Free ◽  
Scale Expansion ◽  
Free Media

Evaluating the use of Terumo Quantum® cell expansion system for large scale expansion of mesenchymal stem (stromal) cells in xeno- and serum-free media

Cytotherapy ◽  
2017 ◽  
Vol 19 (5) ◽  
pp. S163-S164
Author(s):  
S. Khan ◽  
M. Salkhordeh ◽  
J. Schafhauser ◽  
F. Hussein ◽  
S. Hodgins ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Cell Expansion ◽  
Large Scale ◽  
Serum Free ◽  
Expansion System ◽  
Scale Expansion ◽  
Free Media

scholarly journals Large-scale gene expression profiling data of bone marrow stromal cells from osteoarthritic donors

Data in Brief ◽  
2016 ◽  
Vol 8 ◽  
pp. 545-548 ◽  
Author(s):  
Maik Stiehler ◽  
Juliane Rauh ◽  
Cody Bünger ◽  
Angela Jacobi ◽  
Corina Vater ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Marrow ◽  
Gene Expression Profiling ◽  
Stromal Cells ◽  
Expression Profiling ◽  
Bone Marrow Stromal Cells ◽  
Large Scale ◽  
Marrow Stromal Cells

Large-scale room-temperature synthesis and optical properties of perovskite-related Cs4PbBr6 fluorophores

2016 ◽  
Vol 4 (45) ◽  
pp. 10646-10653 ◽  
Author(s):  
Daqin Chen ◽  
Zhongyi Wan ◽  
Xiao Chen ◽  
Yongjun Yuan ◽  
Jiasong Zhong
Keyword(s):  
Optical Properties ◽  
Large Scale ◽  
Room Temperature ◽  
Interface Reaction ◽  
Phase System ◽  
Temperature Synthesis ◽  
Two Phase ◽  
Room Temperature Synthesis

An inhomogeneous interface reaction in a liquid–liquid immiscible two-phase system is developed to realize the large-scale room-temperature synthesis of perovskite-related Cs4PbBr6 fluorophores.

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