Enhancement of galactose consumption rate in Saccharomyces cerevisiae CEN.PK2-1 by CRISPR Cas9 and adaptive evolution for fermentation of Kappaphycus alvarezii hydrolysate

2019 ◽  
Vol 297 ◽  
pp. 78-84 ◽  
Author(s):  
In Yung Sunwoo ◽  
Pailin Sukwong ◽  
Deok Yeol Jeong ◽  
Soo Rin Kim ◽  
Gwi-Teak Jeong ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Adaptive Evolution ◽  
Consumption Rate ◽  
Kappaphycus Alvarezii

scholarly journals Construction of fast xylose-fermenting yeast based on industrial ethanol-producing diploid Saccharomyces cerevisiae by rational design and adaptive evolution

2013 ◽  
Vol 13 (1) ◽  
pp. 110 ◽  
Author(s):  
Liuyang Diao ◽  
Yingmiao Liu ◽  
Fenghui Qian ◽  
Junjie Yang ◽  
Yu Jiang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Adaptive Evolution ◽  
Rational Design

scholarly journals Improvement of L-Arabinose Fermentation by Modifying the Metabolic Pathway and Transport inSaccharomyces cerevisiae

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Chengqiang Wang ◽  
Yu Shen ◽  
Yanyan Zhang ◽  
Fan Suo ◽  
Jin Hou ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Recombinant Strain ◽  
Consumption Rate ◽  
Specific Growth ◽  
Ethanol Yield ◽  
Maximum Specific Growth Rate ◽  
Dry Cell Weight ◽  
Utilization Pathway ◽  
Dry Cell ◽  
Arabinose Fermentation

The L-arabinose utilization pathway was established inSaccharomyces cerevisiae, by expressing the codon-optimizedaraA,araB, andaraDgenes ofLactobacillus plantarum. After overexpressing theTAL1,TKL1,RPE1,RKI1, andGAL2genes and adaptive evolution, the L-arabinose utilization of the recombinant strain became efficient. The resulting strain displayed a maximum specific growth rate of 0.075 h−1, a maximum specific L-arabinose consumption rate of 0.61 g h−1 g−1dry cell weight, and a promising ethanol yield of 0.43 g g−1from L-arabinose fermentation.

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