Determination of geniposidic acid in rat plasma by LC–MS/MS and its application to in vivo pharmacokinetic studies

Objective: Recently, we reported newly synthesized 5H-benzo[2,3][1,4]oxazepino[5,6-b]indole) derivatives and proved their cytotoxicity against hepatocellular carcinoma specific Hep-G2 cell lines. We attempted herein to describe a reversed-phase high-performance liquid chromatographic method for the determination of three most active compounds 6a, 10a, and 15a in rat plasma to predict their pharmacokinetics parameters before in vivo study.Methods: A rapid and sensitive reversed-phase high-performance liquid chromatographic was employed for the determination of 6a, 10a, and 15a in rat plasma. Each compound was separated by a gradient elution of acetonitrile and water with 1 mL/min flow rate. The detector was set at 270, 285, and 275 nm for 6a, 10a, and 15a and the recorded elution times were 2.00, 2.87, and 1.88 min, respectively.Results: The calibration curve was linear with R2 of 0.938, 0.875, and 0.923 over the concentration range of 0.1–50 μg/mL. The inter- and intra-day variations of the assay were lower than 12.26%; the average recovery of 6a, 10a, and 15a was 97.31, 92.56, and 95.23 % with relative standard deviation of 2.12%, 3.25%, and 2.28%, respectively. The Cmax and Tmax were ~ 46.34, 18.56, and 25.65 μg/mL and 2.0, 4.0, and 4.0 h for 6a, 10a, and 15a, respectively, which indicate a robust method of detection in the present experiment.Conclusion: The study suggests that all of the three compounds have a lower rate of absorption, higher volume of distribution, and lower clearance rate, indicating good therapeutic response for in vivo activity.

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Determination of visnagin in rat plasma by liquid chromatography with tandem mass spectrometry and its application to in vivo pharmacokinetic studies

Journal of Chromatography B ◽

10.1016/j.jchromb.2009.01.014 ◽

2009 ◽

Vol 877 (7) ◽

pp. 653-656 ◽

Cited By ~ 5

Author(s):

Pattaraporn Vanachayangkul ◽

Veronika Butterweck ◽

Reginald F. Frye

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Tandem Mass Spectrometry ◽

Rat Plasma ◽

Pharmacokinetic Studies ◽

Tandem Mass

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Cloud-Point Extraction Combined with Liquid Chromatography for the Determination of Ergosterol, a Natural Product with Diuretic Activity, in Rat Plasma, Urine, and Faeces

Journal of Analytical Methods in Chemistry ◽

10.1155/2013/479056 ◽

2013 ◽

Vol 2013 ◽

pp. 1-8 ◽

Cited By ~ 5

Author(s):

Dan-Qian Chen ◽

Jun-Min An ◽

Ya-Long Feng ◽

Ting Tian ◽

Xiang-Yang Qin ◽

...

Keyword(s):

Liquid Chromatography ◽

Cloud Point ◽

Cloud Point Extraction ◽

Rat Plasma ◽

Ionic Surfactant ◽

Pharmacokinetic Studies ◽

Triton X

Ergosterol from many medicinal fungi has been demonstrated to possess a variety of pharmacological activitiesin vivoandin vitro. A new method based on cloud-point extraction has been developed, optimized and validated for the determination of ergosterol in rat plasma, urine and faeces by liquid chromatography. The non-ionic surfactant Triton X-114 was chosen as the extract solvent. The chromatographic separation was performed on an Inertsil ODS-3 analytical column with a mobile phase consisting of methanol and water (98 : 2, v/v) at a flow rate of 1 mL/min. The methodology was validated completely. The results indicated good performance in terms of specificity, linearity, detection and quantification limits, precision and accuracy. The method was successfully applied to the pharmacokinetic studies of ergosterol in rats. The results indicate that the ergosterol levels in feces are much higher than those in plasma and urine of the rat.

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Simultaneous determination of vasicine and its major metabolites in rat plasma by UPLC-MS/MS and its application to in vivo pharmacokinetic studies

RSC Advances ◽

10.1039/c5ra12547b ◽

2015 ◽

Vol 5 (95) ◽

pp. 78336-78351 ◽

Cited By ~ 7

Author(s):

Wei Liu ◽

Dandan He ◽

Yudan Zhu ◽

Xuemei Cheng ◽

Hao Xu ◽

...

Keyword(s):

Simultaneous Determination ◽

Pharmacokinetic Study ◽

Rat Plasma ◽

Pharmacokinetic Studies ◽

Butyrylcholinesterase Activity

An UPLC-MS/MS method was developed to simultaneously determinate vasicine and its main metabolites and applied to the pharmacokinetic study. In addition, the anti-butyrylcholinesterase activity of component in plasma was evaluatedin vitro.

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