Separation of porcine parvovirus from bovine serum albumin using PEG–salt aqueous two-phase system

2014 ◽  
Vol 967 ◽  
pp. 118-126 ◽  
Author(s):  
K. Saagar Vijayaragavan ◽  
Amna Zahid ◽  
Jonathan W. Young ◽  
Caryn L. Heldt
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Phase System ◽  
Porcine Parvovirus ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Interfacial partitioning behaviour of bovine serum albumin in polymer-salt aqueous two-phase system

2013 ◽  
Vol 934 ◽  
pp. 71-78 ◽  
Author(s):  
Yin Hui Chow ◽  
Yee Jiun Yap ◽  
Mohd. Shamsul Anuar ◽  
Bimo Ario Tejo ◽  
Arbakariya Ariff ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Influence of Phase Compositions, pH and Temperature on Bovine Serum Albumin Partitioning in Aqueous Biphasic System

2010 ◽  
Vol 5 (1) ◽  
Author(s):  
Perumalsamy Muthiah ◽  
Bharat Kumar Pampana
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Sodium Citrate ◽  
Phase System ◽  
Two Phase ◽  
Lower Phase ◽  
Peg 4000 ◽  
Aqueous Biphasic ◽  
Tie Line

The partitioning of bovine serum albumin (BSA) was investigated in polyethylene glycol (PEG) 4000-sodium citrate system at 308K and 318K with varying pHs and tie line lengths (TLL). The systems were prepared at a pH of 5.2, 7.1 and 9.2 with different TLL compositions. The affinity of the BSA for the lower phase increases with an increase in pH, which is due to an increase of trivalent to divalent citrate ions in the two phase system. The affinity of the BSA to the upper phase increases with an increase in TLL, which is due to the increase in available free volume in the upper phase. As the temperature increases, the partition coefficient of BSA shows increment.

Partitioning of whey proteins, bovine serum albumin and porcine insulin in aqueous two-phase systems

2000 ◽  
Vol 743 (1-2) ◽  
pp. 235-239 ◽  
Author(s):  
José G.L.F Alves ◽  
Lucy D.A Chumpitaz ◽  
Luiza H.M da Silva ◽  
Telma T Franco ◽  
Antonio J.A Meirelles
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Whey Proteins ◽  
Porcine Insulin ◽  
Two Phase ◽  
Aqueous Two Phase Systems ◽  
Phase Systems
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