Partitioning of bovine serum albumin in an aqueous two-phase system: optimization of partition coefficient

2000 ◽  
Vol 743 (1-2) ◽  
pp. 259-262 ◽  
Author(s):  
Ufuk Gündüz
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Partition Coefficient ◽  
Bovine Serum ◽  
System Optimization ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Separation of porcine parvovirus from bovine serum albumin using PEG–salt aqueous two-phase system

2014 ◽  
Vol 967 ◽  
pp. 118-126 ◽  
Author(s):  
K. Saagar Vijayaragavan ◽  
Amna Zahid ◽  
Jonathan W. Young ◽  
Caryn L. Heldt
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Phase System ◽  
Porcine Parvovirus ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Interfacial partitioning behaviour of bovine serum albumin in polymer-salt aqueous two-phase system

2013 ◽  
Vol 934 ◽  
pp. 71-78 ◽  
Author(s):  
Yin Hui Chow ◽  
Yee Jiun Yap ◽  
Mohd. Shamsul Anuar ◽  
Bimo Ario Tejo ◽  
Arbakariya Ariff ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

Influence of Phase Compositions, pH and Temperature on Bovine Serum Albumin Partitioning in Aqueous Biphasic System

2010 ◽  
Vol 5 (1) ◽  
Author(s):  
Perumalsamy Muthiah ◽  
Bharat Kumar Pampana
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Sodium Citrate ◽  
Phase System ◽  
Two Phase ◽  
Lower Phase ◽  
Peg 4000 ◽  
Aqueous Biphasic ◽  
Tie Line

The partitioning of bovine serum albumin (BSA) was investigated in polyethylene glycol (PEG) 4000-sodium citrate system at 308K and 318K with varying pHs and tie line lengths (TLL). The systems were prepared at a pH of 5.2, 7.1 and 9.2 with different TLL compositions. The affinity of the BSA for the lower phase increases with an increase in pH, which is due to an increase of trivalent to divalent citrate ions in the two phase system. The affinity of the BSA to the upper phase increases with an increase in TLL, which is due to the increase in available free volume in the upper phase. As the temperature increases, the partition coefficient of BSA shows increment.

Partition coefficient of bovine serum albumin in two-phase aqueous systems

1988 ◽  
Vol 2 (1) ◽  
pp. 41-46
Author(s):  
S. B. Sawant ◽  
J. B. Joshi ◽  
S. K. Sikdar
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Partition Coefficient ◽  
Bovine Serum ◽  
Aqueous Systems ◽  
Two Phase

scholarly journals Optimization of Aqueous Two-Phase System (ATPS) of Recombinant Bromelain by Response Surface Methodology

2018 ◽  
Vol 7 (4.30) ◽  
pp. 377
Author(s):  
Zatul Iffah Mohd Arshad ◽  
Azura Amid
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Partition Coefficient ◽  
Response Surface ◽  
Potassium Phosphate ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Crude Sample ◽  
Face Centered

Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel.

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