Influence of Phase Compositions, pH and Temperature on Bovine Serum Albumin Partitioning in Aqueous Biphasic System

2010 ◽  
Vol 5 (1) ◽  
Author(s):  
Perumalsamy Muthiah ◽  
Bharat Kumar Pampana
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Sodium Citrate ◽  
Phase System ◽  
Two Phase ◽  
Lower Phase ◽  
Peg 4000 ◽  
Aqueous Biphasic ◽  
Tie Line

The partitioning of bovine serum albumin (BSA) was investigated in polyethylene glycol (PEG) 4000-sodium citrate system at 308K and 318K with varying pHs and tie line lengths (TLL). The systems were prepared at a pH of 5.2, 7.1 and 9.2 with different TLL compositions. The affinity of the BSA for the lower phase increases with an increase in pH, which is due to an increase of trivalent to divalent citrate ions in the two phase system. The affinity of the BSA to the upper phase increases with an increase in TLL, which is due to the increase in available free volume in the upper phase. As the temperature increases, the partition coefficient of BSA shows increment.

Separation of porcine parvovirus from bovine serum albumin using PEG–salt aqueous two-phase system

2014 ◽  
Vol 967 ◽  
pp. 118-126 ◽  
Author(s):  
K. Saagar Vijayaragavan ◽  
Amna Zahid ◽  
Jonathan W. Young ◽  
Caryn L. Heldt
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Phase System ◽  
Porcine Parvovirus ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Interfacial partitioning behaviour of bovine serum albumin in polymer-salt aqueous two-phase system

2013 ◽  
Vol 934 ◽  
pp. 71-78 ◽  
Author(s):  
Yin Hui Chow ◽  
Yee Jiun Yap ◽  
Mohd. Shamsul Anuar ◽  
Bimo Ario Tejo ◽  
Arbakariya Ariff ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System

scholarly journals Efficacy of Alcohol/sugar Aqueous Biphasic System on Partition of Bovine Serum Albumin

2020 ◽  
Author(s):  
Yin Hui Chow ◽  
Alagan Sahlini ◽  
Hui-Suan Ng ◽  
Chi-Wei Lan
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Phase 1 ◽  
Biphasic System ◽  
Bottom Phase ◽  
Aqueous Biphasic System ◽  
Model Protein ◽  
Aqueous Biphasic ◽  
The Fourier Transform

Abstract A green bio-separation alternative can be performed with a non-toxic and biodegradable aqueous biphasic system (ABS) composed of short-chain aliphatic alcohol-based top phase (1-propanol and 2-propanol) and carbohydrate-based bottom phase (glucose, sucrose, and maltose). A model protein, bovine serum albumin (BSA) was adopted to determine the effects of types and concentration of phase-forming components; protein concentration; and system pH on the protein partition efficiency in the ABS. Results showed that the 1-propanol/maltose ABS gave an overall better partition efficiency of BSA to the alcohol-rich top phase compared to the 1-propanol/sucrose ABS, 1-propanol/glucose ABS, and 2-propanol/sugar ABS attributed to the lower hydrophilicity of 1-propanol and the stronger sugaring-out effect exerted by the maltose. A maximum partition coefficient (K) of 20.01 ± 0.05 and recovery yield (Y) of 95.42% ± 0.01 of BSA were obtained with the 35% (w/w) 1-propanol/22% (w/w) maltose ABS at pH 5.0 which contained 10% (w/w) BSA. The K and Y of BSA in 1-propanol/maltose ABS was further enhanced with the addition of 3% (w/w) of ionic liquids, 1-butyl-3-methylimidazolium bromide ([Bmim]Br) as the adjuvants which provides the protein stabilizing effect. The Fourier Transform Infrared Spectrum (FTIR) analysis revealed that the protein structure remained unaltered upon the separation process.

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