Comprehensive characterization of the in vitro and in vivo metabolites of limonin in human samples using LC-Q-TOF/MS

2017 ◽  
Vol 1068-1069 ◽  
pp. 226-232 ◽  
Author(s):  
Shijia Liu ◽  
Peidong Chen ◽  
Nongshan Zhang ◽  
Luning Sun ◽  
Guoliang Dai ◽  
...  
Keyword(s):  
Human Samples ◽  
Comprehensive Characterization ◽  
Tof Ms

scholarly journals A novel strategy for the characterization of glaucocalyxin A metabolites in vivo and in vitro by UHPLC-Q-TOF-MS based on DDA and DIA data acquisitions

RSC Advances ◽  
2020 ◽  
Vol 10 (18) ◽  
pp. 10431-10446
Author(s):  
Wenjing Sun ◽  
Yiran Jin ◽  
Shuai Guan ◽  
Mengxin Yang ◽  
Miaoting Zhang ◽  
...  
Keyword(s):  
Process Flow ◽  
Experimental Process ◽  
Novel Strategy ◽  
Tof Ms

The experimental process flow.

scholarly journals Design and Comprehensive Characterization of Tetramethylpyrazine (TMP) for Targeted Lung Delivery as Inhalation Aerosols in Pulmonary Hypertension (PH): In Vitro Human Lung Cell Culture and In Vivo Efficacy

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 427
Author(s):  
Priya Muralidharan ◽  
Maria F. Acosta ◽  
Alexan I. Gomez ◽  
Carissa Grijalva ◽  
Haiyang Tang ◽  
...  
Keyword(s):  
Human Lung ◽  
Antioxidant Properties ◽  
Dry Powder Inhaler ◽  
Chinese Herbs ◽  
Dry Powder ◽  
Comprehensive Characterization ◽  
Spray Dried

This is the first study reporting on the design and development innovative inhaled formulations of the novel natural product antioxidant therapeutic, tetramethylpyrazine (TMP), also known as ligustrazine. TMP is obtained from Chinese herbs belonging to the class of Ligusticum. It is known to have antioxidant properties. It can act as a Nrf2/ARE activator and a Rho/ROCK inhibitor. The present study reports for the first time on the comprehensive characterization of raw TMP (non-spray dried) and spray dried TMP in a systematic manner using thermal analysis, electron microscopy, optical microscopy, and Raman spectroscopy. The in vitro aerosol dispersion performance of spray dried TMP was tested using three different FDA-approved unit-dose capsule-based human dry powder inhaler devices. In vitro human cellular studies were conducted on pulmonary cells from different regions of the human lung to examine the biocompatibility and non-cytotoxicity of TMP. Furthermore, the efficacy of inhaled TMP as both liquid and dry powder inhalation aerosols was tested in vivo using the monocrotaline (MCT)-induced PH rat model.

In Vitro and In Vivo Primary Metabolic Characterization of F18, a Novel Histone Deacetylase-6 (HDAC6) Inhibitor, Using UHPLC–QqQ–MS/MS and Q-TOF–MS Methods

2016 ◽  
Vol 79 (21-22) ◽  
pp. 1479-1490 ◽  
Author(s):  
Xiaobin Li ◽  
Minghai Tang ◽  
Hairong Wang ◽  
Liang Ma ◽  
Haoyu Ye ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Histone Deacetylase 6 ◽  
Hdac6 Inhibitor ◽  
Tof Ms

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

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