Bone repair of critical size defects treated with mussel powder associated or not with bovine bone graft: Histologic and histomorphometric study in rat calvaria

Abstract Introduction The use of bone substitutes in grafting procedures as an alternative of the use of autogenous bone graft has been indicated, however, the direct comparison between these biomaterials has been little explored. Objective To evaluate the effect of different osteoconductive bone substitutes on the bone repair in critical-sized defects (CSDs) in rat calvaria. Material and method One CSD with an 8 mm diameter was made in each of the 40 rats used in this study. The animals were randomly allocated into 5 groups (n=8), according to the type of bone substitute used to fill the CSD: COA (Coagulum); AUT (autogenous bone); DBB (deproteinized bovine bone graft); HA/TCP (biphasic ceramic composed of hydroxyapatite and β-phosphate tricalcium); and TCP (β-phosphate tricalcium). A microtomographic analysis was performed to evaluate the remaining defect linear length (DLL) of the CSD and the volume of the mineralized tissues (MT) within the CSD at 3, 7, 15 and 30 days after the surgical procedure. In addition, a histometric analysis was performed to evaluate the composition of the repaired bone tissue (% Bone and % Biomaterial) at the 30-day period. Result It was shown that the COA had the lowest DLL and MT within the CSD. In addition, the COA presented the highest % of bone in CSD. The DBB had a higher MT and a higher % of bone substitute particles in the CSD than the AUT and TCP groups. The DBB and AUT groups presented higher % of bone in the CSD than the TCP group. Conclusion The use of the DBB promoted a better pattern of bone volume gain and formation compared to TCP and HA / TCP but was biologically inferior to the AUT.

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Immunoexpression of PPAR-? and Osteocalcin Proteins for Bone Repair of Critical-Size Defects Treated with Fragmented Autogenous Abdominal Adipose Tissue Graft

Brazilian Dental Journal ◽

10.1590/0103-6440201302430 ◽

2014 ◽

Vol 25 (3) ◽

pp. 179-185 ◽

Cited By ~ 4

Author(s):

Tatiana Miranda Deliberador ◽

Allan Fernando Giovanini ◽

Tertuliano Ricardo Lopes ◽

João César Zielak ◽

Alexandre Moro ◽

...

Keyword(s):

Adipose Tissue ◽

Bone Repair ◽

Collagen Fiber ◽

Critical Size ◽

Fiber Bundles ◽

Abdominal Adipose Tissue ◽

Tissue Graft ◽

Ppar Γ ◽

Critical Size Defects ◽

Rat Calvaria

Immunoexpression of PPAR-γ and osteocalcin proteins was evaluated for bone repair of critical-size defects (CSDs), created in rat calvaria (n=42) and treated with fragmented abdominal autogenous adipose tissue graft. Three groups (n=14) were formed: C (control - blood clot), AB (autogenous bone) and AT (fragmented adipose tissue). The groups were divided into subgroups (n=7) for euthanasia at 30 and 90 days. Histological and immunohistochemical analyses were performed. Data were subjected to descriptive statistics (mode). A complete bone closure was observed in Group AB 90 days after surgery. In Group C, repair was achieved by the formation of collagen fiber bundles oriented parallel to the wound surface at both post-surgery periods. In Group AT the type of healing was characterized by dense connective tissue containing collagen fiber bundles arranged amidst the remaining adipose tissue, with rare heterotopic bone formation associated with fibrosis and different types of tissue necrosis. Immunostaining of PPAR-γ was not observed in any specimen from Groups C and AB. In Group AT, the immunostaining of PPAR-γ was more evident 30 days after surgery. Immunostaining of osteocalcin was present in all groups and at both postoperative periods. The fragmented autogenous abdominal adipose tissue graft did not favor the repair of critical-size bone defects created surgically in rat calvaria as evidenced by the positive immunostaining of PPAR-γ protein and the negative immunostaining of osteocalcin in the osteoblast-like cells and bone matrix.

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Effects of oestrogen deficiency and 17β-estradiol therapy on bone healing in calvarial critical size defects treated with bovine bone graft

Archives of Oral Biology ◽

10.1016/j.archoralbio.2015.01.009 ◽

2015 ◽

Vol 60 (4) ◽

pp. 631-641 ◽

Cited By ~ 6

Author(s):

Juliano Milanezi de Almeida ◽

Alvaro Francisco Bosco ◽

Paula Lazilha Faleiros ◽

Victor Fabrizio Cabrera Pazmiño ◽

Ana Cristina Murakawa ◽

...

Keyword(s):

Bone Graft ◽

Bone Healing ◽

Critical Size ◽

Bovine Bone ◽

Critical Size Defects ◽

Oestrogen Deficiency ◽

17Β Estradiol

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Influence of the ratio of particulate autogenous bone graft/platelet-rich plasma on bone healing in critical-size defects: A histologic and histometric study in rat calvaria

Journal of Orthopaedic Research® ◽

10.1002/jor.21027 ◽

2009 ◽

pp. n/a-n/a ◽

Cited By ~ 4

Author(s):

Maria J. Nagata ◽

Michel Messora ◽

Nat��lia Pola ◽

Nat��lia Campos ◽

Rafael Vieira ◽

...

Keyword(s):

Bone Graft ◽

Bone Healing ◽

Critical Size ◽

Platelet Rich Plasma ◽

Autogenous Bone Graft ◽

Autogenous Bone ◽

Critical Size Defects ◽

Rat Calvaria

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Evaluation of bone repair of critical size defects treated with simvastatin-loaded poly(lactic-co-glycolic acid) microspheres in rat calvaria

Journal of Biomaterials Applications ◽

10.1177/0885328214550897 ◽

2014 ◽

Vol 29 (7) ◽

pp. 965-976 ◽

Cited By ~ 12

Author(s):

Lorraine B Ferreira ◽

Vivian Bradaschia-Correa ◽

Mariana M Moreira ◽

Natasha DM Marques ◽

Victor E Arana-Chavez

Keyword(s):

Bone Repair ◽

Glycolic Acid ◽

Critical Size ◽

Critical Size Defects ◽

Rat Calvaria

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Natural Bovine Anorganic Apatite and Collagen Presents Osteoconductivity and Contribute to Bone Repair of Rat Calvaria Critical Size Defect

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.396-398.249 ◽

2008 ◽

Vol 396-398 ◽

pp. 249-252 ◽

Cited By ~ 2

Author(s):

I.I.C. Silva ◽

S. Pimentel-Soares ◽

Rafael C. Bittencourt ◽

José Mauro Granjeiro

Keyword(s):

Bone Repair ◽

Critical Size ◽

Blood Clot ◽

Autogenous Bone ◽

Histopathological Analysis ◽

Critical Size Defect ◽

Critical Size Defects ◽

Rat Calvaria ◽

Bovine Xenograft

The aim of this study was verify the biological efficacy of the use of a xenograft for bone loss therapy. Blood clot, particulate autogenous bone or anorganic bovine xenograft filled critical size defects (CSD) in rat calvaria (8mm diameter). After 0, 7, 30 and 90 days the animals were killed and macroscopic, radiographic and histopathological analysis were conducted. Although no treatment promoted the total closure of bone defect, autogenous bone group had better bone repair after 90 days, followed by xenograft group that exhibited direct bone neoformation onto, and around, the particles confirming its osteoconductivity. In conclusion, the xenograft tested in vivo showed biocompatibility, biodegradability and osteoconductive properties in rat calvaria CSD.

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