scholarly journals Bone repair induced by different bone graft substitutes in critical-sized defects in rat calvaria

2019 ◽  
Vol 48 ◽  
Author(s):  
Mauricio Andrés Tinajero ARONI ◽  
Paulo Firmino da COSTA NETO ◽  
Guilherme José Pimentel Lopes de OLIVEIRA ◽  
Rosemary Adriana Chiérici MARCANTONIO ◽  
Elcio MARCANTONIO JUNIOR
Keyword(s):  
Bone Graft ◽  
Bone Substitute ◽  
Bone Repair ◽  
Bone Substitutes ◽  
Autogenous Bone ◽  
Bovine Bone ◽  
Rat Calvaria ◽  
Mineralized Tissues ◽  
Bone Graft Substitutes ◽  
Biphasic Ceramic

Abstract Introduction The use of bone substitutes in grafting procedures as an alternative of the use of autogenous bone graft has been indicated, however, the direct comparison between these biomaterials has been little explored. Objective To evaluate the effect of different osteoconductive bone substitutes on the bone repair in critical-sized defects (CSDs) in rat calvaria. Material and method One CSD with an 8 mm diameter was made in each of the 40 rats used in this study. The animals were randomly allocated into 5 groups (n=8), according to the type of bone substitute used to fill the CSD: COA (Coagulum); AUT (autogenous bone); DBB (deproteinized bovine bone graft); HA/TCP (biphasic ceramic composed of hydroxyapatite and β-phosphate tricalcium); and TCP (β-phosphate tricalcium). A microtomographic analysis was performed to evaluate the remaining defect linear length (DLL) of the CSD and the volume of the mineralized tissues (MT) within the CSD at 3, 7, 15 and 30 days after the surgical procedure. In addition, a histometric analysis was performed to evaluate the composition of the repaired bone tissue (% Bone and % Biomaterial) at the 30-day period. Result It was shown that the COA had the lowest DLL and MT within the CSD. In addition, the COA presented the highest % of bone in CSD. The DBB had a higher MT and a higher % of bone substitute particles in the CSD than the AUT and TCP groups. The DBB and AUT groups presented higher % of bone in the CSD than the TCP group. Conclusion The use of the DBB promoted a better pattern of bone volume gain and formation compared to TCP and HA / TCP but was biologically inferior to the AUT.

Bone repair of critical size defects treated with mussel powder associated or not with bovine bone graft: Histologic and histomorphometric study in rat calvaria

2014 ◽  
Vol 42 (6) ◽  
pp. 738-743 ◽  
Author(s):  
Daniel Rizzo Trotta ◽  
Clayton Gorny ◽  
João César Zielak ◽  
Carla Castiglia Gonzaga ◽  
Allan Fernando Giovanini ◽  
...  
Keyword(s):  
Bone Graft ◽  
Bone Repair ◽  
Critical Size ◽  
Bovine Bone ◽  
Histomorphometric Study ◽  
Critical Size Defects ◽  
Rat Calvaria

scholarly journals N-acetyl Cysteine Alleviates Cytotoxicity of Bone Substitute

2010 ◽  
Vol 89 (4) ◽  
pp. 411-416 ◽  
Author(s):  
M. Yamada ◽  
T. Ueno ◽  
H. Minamikawa ◽  
N. Sato ◽  
F. Iwasa ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bone Substitute ◽  
Bone Substitutes ◽  
Amino Acid Derivative ◽  
Bovine Bone ◽  
Intracellular Ros ◽  
Cytokine Levels ◽  
Acetyl Cysteine ◽  
And Function ◽  
Bone Particles

Lack of cytocompatibility in bone substitutes impairs healing in surrounding bone. Adverse biological events around biomaterials may be associated with oxidative stress. We hypothesized that a clinically used inorganic bone substitute is cytotoxic to osteoblasts due to oxidative stress and that N-acetyl cysteine (NAC), an antioxidant amino acid derivative, would detoxify such material. Only 20% of rat calvaria osteoblasts were viable when cultured on commercial deproteinized bovine bone particles for 24 hr, whereas this percentage doubled on bone substitute containing NAC. Intracellular ROS levels markedly increased on and under bone substitutes, which were reduced by prior addition of NAC to materials. NAC restored suppressed alkaline phosphatase activity in the bone substitute. Proinflammatory cytokine levels from human osteoblasts on the bone substitute decreased by one-third or more with addition of NAC. NAC alleviated cytotoxicity of the bone substitute to osteoblastic viability and function, implying enhanced bone regeneration around NAC-treated inorganic biomaterials.

A Novelty of Synthetic Hydroxyapatite from Cockle Shell and Characterization

2015 ◽  
Vol 1087 ◽  
pp. 429-433 ◽  
Author(s):  
Rusnah Mustaffa ◽  
Mohd Reusmaazran Mohd Yusof ◽  
Yusof Abdullah
Keyword(s):  
Bone Graft ◽  
Bone Repair ◽  
Artificial Bone ◽  
Synthetic Bone ◽  
Potential Source ◽  
Mineral Constituent ◽  
Bone Graft Substitutes ◽  
Synthetic Hydroxyapatite ◽  
Anadara Granosa ◽  
Cockle Shell

In Malaysia recently, it was found that cockle shell (Anadara granosa) is a potential source of biomaterial for bone repair. It is the most abundant sea species cultured in Malaysia. A possible advantage of using cockle shell as a biomaterial is that they may act as an antilog of calcium carbonate. Malaysian Nuclear Agency took this challenge to develop synthetic bone graft from natural cockle shell. To date, the artificial bone graft substitutes developed from hydroxyapatite (Ca10(PO4)6(OH)2) a bio ceramic is similar to the mineral constituent of human bone. The structure and the composition of hydroxyapatite (HA) are similar to the mineral phase of bone and, its bioactivity and biocompatibility makes it a preferred bone graft.

scholarly journals Comparison of Bone Graft Healing Between Autogenous Bone, Blood Clot and Anorganic Bovine Bone Matrix: Radiographic and Histological Analyses

2013 ◽  
Vol 31 (4) ◽  
pp. 1257-1262
Author(s):  
Sergio Olate ◽  
Henrique Duque Netto ◽  
Leandro E Klüppel ◽  
Bélgica Vásquez ◽  
Maria das Graças A Miranda Chaves ◽  
...  
Keyword(s):  
Bone Graft ◽  
Blood Clot ◽  
Bone Matrix ◽  
Autogenous Bone ◽  
Bovine Bone ◽  
Graft Healing

Morphometric evaluation of the repair of critical-size defects using demineralized bovine bone and autogenous bone grafts in rat calvaria

2008 ◽  
Vol 19 (8) ◽  
pp. 749-754 ◽  
Author(s):  
Rodrigo Cardoso de Oliveira ◽  
Fabio Henrique Gelonese de Oliveira ◽  
Tânia Mary Cestari ◽  
Rumio Taga ◽  
José Mauro Granjeiro
Keyword(s):  
Critical Size ◽  
Bone Grafts ◽  
Autogenous Bone ◽  
Bovine Bone ◽  
Critical Size Defects ◽  
Rat Calvaria ◽  
Morphometric Evaluation ◽  
Autogenous Bone Grafts

Evaluation of bone healing with eggshell-derived bone graft substitutes in rat calvaria: A pilot study

2008 ◽  
Vol 87A (1) ◽  
pp. 203-214 ◽  
Author(s):  
Jin-Woo Park ◽  
Sang-Ryul Bae ◽  
Jo-Young Suh ◽  
Dong-Hee Lee ◽  
Sang-Hyun Kim ◽  
...  
Keyword(s):  
Pilot Study ◽  
Bone Graft ◽  
Bone Healing ◽  
Rat Calvaria ◽  
Bone Graft Substitutes

scholarly journals Morphology of autogenous bone graft and castor oil polyurethane in the infraorbital rim of rabbits: a comparative study

2006 ◽  
Vol 21 (5) ◽  
pp. 341-347 ◽  
Author(s):  
José Carlos Garcia de Mendonça ◽  
Rafael De Rossi ◽  
Celso Massaschi Inouye ◽  
Diego Rodrigo Paulillo Bazan ◽  
João Carlos Castro Monteiro ◽  
...  
Keyword(s):  
Bone Graft ◽  
Castor Oil ◽  
Bone Repair ◽  
Bone Defects ◽  
Autogenous Bone Graft ◽  
Autogenous Bone ◽  
Control Group ◽  
Cell Nuclei ◽  
Zygomatic Bone ◽  
Chi Squared

PURPOSE: Morphological study comparing castor oil polyurethane and autogenous bone graft to repair bone defect in zygomatic bone of rabbits. METHODS: Twenty-four adult, male New Zealand rabbits were randomly distributed between two groups of twelve. Bone defects of 5mm in diameter were cut through the zygomatic bone and filled with polyurethane discs in the experimental group or autogenous bone harvested from the tibia in the control group. Animals were sacrificed after 30, 60 or 90 days, and the zygomatic bones were macro- and microscopically analyzed. Student's, Fisher's, chi-squared and McNemar's tests were used for statistical analysis. RESULTS: Both the castor oil polyurethane and the autograft adapted well to the defect, with no need for fixation. Fibrous connective tissue encapsulated the polyurethane, but no inflammation or giant cell reaction was observed. Acidophilic and basophilic areas were observed inside the micropores of the polyurethane, suggesting cell nuclei. After 90 days, bone repair with a lamellar pattern of organization was observed in the control group. CONCLUSION: The castor oil polyurethane was biocompatible and did not cause inflammation. It may be considered an alternative to fill bone defects.

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