Organic–inorganic hybrid-nanocarrier of siRNA constructing through the self-assembly of calcium phosphate and PEG-based block aniomer

A family of soluble organic–inorganic hybrid doughnut-like anions has been prepared by the self-assembly of polyoxovanadate anions and 1,3-benzenedicarboxylate linkers.

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Fabrication of enamel-mimicking mineralization composite coating induced by electrolytic deposition (ELD) system

MRS Proceedings ◽

10.1557/proc-1008-t04-01 ◽

2007 ◽

Vol 1008 ◽

Author(s):

Yuwei Fan ◽

Rizhi Wang ◽

Janet Moradian-Oldak

Keyword(s):

Calcium Phosphate ◽

Composite Coating ◽

Self Assembly ◽

Tooth Enamel ◽

Composite Coatings ◽

The Self ◽

Electrolytic Deposition ◽

Phosphate Solution ◽

Scanning Electron Micrographs ◽

Nano Composite

AbstractMature tooth enamel does not remodel nor self-repairs after damage. Therefore, in vitro formation of a uniquely ordered composite similar to enamel is of particular interest. In this study we applied a newly developed electrolytic deposition (ELD) system to promote the amelogenin nano-chain self-assembly and simultaneous calcium phosphate crystallization. Composite coatings of amelogenin-calcium phosphate were prepared on a cathode substrate (Si wafer), from an initial pH of 4-5, and calcium and phosphorus containing solution at room temperature. The effects of a recombinant full-length amelogenin (rP172) and truncated protein without the C-terminal (rP148) on the growth and morphology of the calcium phosphate nano-composite were investigated. A potentiostat was used to control the electrochemical parameters.Following the application of electric current, the local pH around the cathode was increased and resulted in the self-assembly of amelogenin that occurred simultaneously with calcium phosphate mineralization. Transmission and scanning electron micrographs of assembled rp172 amelogenin collected from the 25mM sodium phosphate solution during electrodeposition showed uniform nanospheres and nano-chains structures, while the rP148 formed irregular aggregates. SEM observation of the surface of the nano-composite after electrodeposition, revealed organized nano-rod structures in the presence of rP172, while only nano-sized spherical aggregates were seen in the presence of rP148. ATR-IR confirmed that the mineral phases were mainly octacalcium phosphate, but apatite and amorphous calcium phosphate were also present. Nanoindentation was tested on rP172 and rP148 composite coating. The rP172 composite coating exhibited higher elastic modulus and hardness than rP148 composite. The fracture toughness of rP172 composite was comparable to mature enamel. We therefore conclude that the 24 amino acid hydrophilic C-terminal of amelogenin is essential for the self-assembly, and therefore, for calcium phosphate crystal organization. The new electro-deposition system is effective for fabrication of amelogenin/apatite composites with defined organized structures.

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Effects of phosphorylation on the self-assembly of native full-length porcine amelogenin and its regulation of calcium phosphate formation in vitro

Journal of Structural Biology ◽

10.1016/j.jsb.2010.11.006 ◽

2011 ◽

Vol 173 (2) ◽

pp. 250-260 ◽

Cited By ~ 49

Author(s):

Felicitas B. Wiedemann-Bidlack ◽

Seo-Young Kwak ◽

Elia Beniash ◽

Yasuo Yamakoshi ◽

James P. Simmer ◽

...

Keyword(s):

Calcium Phosphate ◽

Self Assembly ◽

Full Length ◽

The Self

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Solid-State Coordination Chemistry: The Self-Assembly of Microporous Organic-Inorganic Hybrid Frameworks Constructed from Tetrapyridylporphyrin and Bimetallic Oxide Chains or Oxide Clusters

Angewandte Chemie International Edition ◽

10.1002/(sici)1521-3773(19991102)38:21<3165::aid-anie3165>3.0.co;2-o ◽

1999 ◽

Vol 38 (21) ◽

pp. 3165-3168 ◽

Cited By ~ 291

Author(s):

Douglas Hagrman ◽

Pamela J. Hagrman ◽

Jon Zubieta

Keyword(s):

Coordination Chemistry ◽

Solid State ◽

Self Assembly ◽

The Self ◽

Inorganic Hybrid ◽

State Coordination

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Formation of calcium phosphate nanostructures under the influence of self-assembling hybrid elastin-like-statherin recombinamers

RSC Advances ◽

10.1039/c6ra01100d ◽

2016 ◽

Vol 6 (37) ◽

pp. 31225-31234 ◽

Cited By ~ 11

Author(s):

M. Hamed Misbah ◽

M. Espanol ◽

Luis Quintanilla ◽

M. P. Ginebra ◽

J. Carlos Rodríguez-Cabello

Keyword(s):

Calcium Phosphate ◽

Self Assembly ◽

Great Influence ◽

The Self ◽

Self Assembling ◽

Phosphate Mineralization

The self-assembly properties of elastin-like-statherin recombinamers have great influence on calcium phosphate mineralization.

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The Nature of Rapidly Extracted Phycocyanin from the Blue-Green Alga Plectonema Boryanum

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100065870 ◽

1971 ◽

Vol 29 ◽

pp. 366-367

Author(s):

M. Kessel ◽

R. MacColl

Keyword(s):

Self Assembly ◽

Analytical Ultracentrifugation ◽

Uranyl Acetate ◽

The Self ◽

Major Protein ◽

Subunit Structure ◽

Blue Green Alga ◽

Thin Sections ◽

Blue Green Algae ◽

Cacodylate Buffer

The major protein of the blue-green algae is the biliprotein, C-phycocyanin (Amax = 620 nm), which is presumed to exist in the cell in the form of distinct aggregates called phycobilisomes. The self-assembly of C-phycocyanin from monomer to hexamer has been extensively studied, but the proposed next step in the assembly of a phycobilisome, the formation of 19s subunits, is completely unknown. We have used electron microscopy and analytical ultracentrifugation in combination with a method for rapid and gentle extraction of phycocyanin to study its subunit structure and assembly.To establish the existence of phycobilisomes, cells of P. boryanum in the log phase of growth, growing at a light intensity of 200 foot candles, were fixed in 2% glutaraldehyde in 0.1M cacodylate buffer, pH 7.0, for 3 hours at 4°C. The cells were post-fixed in 1% OsO4 in the same buffer overnight. Material was stained for 1 hour in uranyl acetate (1%), dehydrated and embedded in araldite and examined in thin sections.

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Interrelationship of the cellular distribution and secretion of regular structure (RS) protein in Bacillus licheniformis nm 105

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100123969 ◽

1992 ◽

Vol 50 (1) ◽

pp. 712-713

Author(s):

Xiaorong Zhu ◽

Richard McVeigh ◽

Bijan K. Ghosh

Keyword(s):

Alkaline Phosphatase ◽

Bacillus Licheniformis ◽

Self Assembly ◽

Gel Electrophoresis ◽

Culture Supernatant ◽

Regular Structure ◽

The Self ◽

Cellular Distribution ◽

Structural Protein ◽

Laboratory Cultures

A mutant of Bacillus licheniformis 749/C, NM 105 exhibits some notable properties, e.g., arrest of alkaline phosphatase secretion and overexpression and hypersecretion of RS protein. Although RS is known to be widely distributed in many microbes, it is rarely found, with a few exceptions, in laboratory cultures of microorganisms. RS protein is a structural protein and has the unusual properties to form aggregate. This characteristic may have been responsible for the self assembly of RS into regular tetragonal structures. Another uncommon characteristic of RS is that enhanced synthesis and secretion which occurs when the cells cease to grow. Assembled RS protein with a tetragonal structure is not seen inside cells at any stage of cell growth including cells in the stationary phase of growth. Gel electrophoresis of the culture supernatant shows a very large amount of RS protein in the stationary culture of the B. licheniformis. It seems, Therefore, that the RS protein is cotranslationally secreted and self assembled on the envelope surface.

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Nanoscale Self-Assembly Using Ion and Electron Beam Techniques: A Rapid Review

MRS Advances ◽

10.1557/adv.2020.349 ◽

2020 ◽

Vol 5 (64) ◽

pp. 3507-3520

Author(s):

Chunhui Dai ◽

Kriti Agarwal ◽

Jeong-Hyun Cho

Keyword(s):

Electron Beam ◽

Self Assembly ◽

Ion Beam ◽

Three Dimensional ◽

The Self ◽

Stress Generation ◽

Rapid Review ◽

High Yield ◽

3D Nanostructures ◽

Self Assembled

AbstractNanoscale self-assembly, as a technique to transform two-dimensional (2D) planar patterns into three-dimensional (3D) nanoscale architectures, has achieved tremendous success in the past decade. However, an assembly process at nanoscale is easily affected by small unavoidable variations in sample conditions and reaction environment, resulting in a low yield. Recently, in-situ monitored self-assembly based on ion and electron irradiation has stood out as a promising candidate to overcome this limitation. The usage of ion and electron beam allows stress generation and real-time observation simultaneously, which significantly enhances the controllability of self-assembly. This enables the realization of various complex 3D nanostructures with a high yield. The additional dimension of the self-assembled 3D nanostructures opens the possibility to explore novel properties that cannot be demonstrated in 2D planar patterns. Here, we present a rapid review on the recent achievements and challenges in nanoscale self-assembly using electron and ion beam techniques, followed by a discussion of the novel optical properties achieved in the self-assembled 3D nanostructures.

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