scholarly journals Relaxation and Simplex mathematical algorithms applied to the study of steady-state electrochemical responses of immobilized enzyme biosensors: Comparison with experiments

2008 ◽  
Vol 616 (1-2) ◽  
pp. 87-98 ◽  
Author(s):  
V. Flexer ◽  
K.F.E. Pratt ◽  
F. Garay ◽  
P.N. Bartlett ◽  
E.J. Calvo
2010 ◽  
Vol 4 (3) ◽  
Author(s):  
Mark I Nelson ◽  
Harvinder S Sidhu ◽  
Adesoji A Adesina

We investigate the behavior of a reaction described by Michaelis-Menten kinetics in an immobilized enzyme reactor (IER). The IER is treated as a well stirred flow reactor, in which the immobilized bounded and unbounded enzyme species are constrained to remain within the reaction vessel. The product species leaves the IER in the reactor outflow. Before the substrate can react with the enzyme, the enzyme must first be activated by absorption of an activator. We use steady state analysis to identify the best operating conditions or the reactor. To this end, we show that the concentration of product is maximized at low residence time whereas the productivity of the reactor is maximized at high residence times.


1978 ◽  
Vol 56 (12) ◽  
pp. 1120-1123 ◽  
Author(s):  
Luigi Toro ◽  
Alfonso Zazzarelli

A new recirculation reactor for kinetic studies of immobilized enzyme is proposed. The reactor consists of two tubes in the same thermostatting jacket. One of the tubes is filled with the immobilized enzyme, the other is filled only with the support. During the approach to steady-state temperature, the circulation is carried out only in the inactive tube. Subsequently, when temperature is stable, the active reactor is used and the solution is not recirculated.


Author(s):  
Odd M. Faltinsen ◽  
Alexander N. Timokha

Steady-state resonant sloshing in a spherical rigid tank due to horizontal harmonic excitations at the lowest natural frequency is classified by combining the nonlinear multimodal method with the Moiseev-Narimanov asymptotics. The theoretical results are validated by comparison with experiments of Sumner & Stofan (1963) and other already published model tests. A good agreement is found for the depth-to-tank radius ratios 0.2 ≤ h ≲ 1 but, when 1 ≲ h ≲ 2, secondary resonance and splashing limits the applicability of the constructed weakly-nonlinear modal theory.


Author(s):  
R. C. Moretz ◽  
G. G. Hausner ◽  
D. F. Parsons

Use of the electron microscope to examine wet objects is possible due to the small mass thickness of the equilibrium pressure of water vapor at room temperature. Previous attempts to examine hydrated biological objects and water itself used a chamber consisting of two small apertures sealed by two thin films. Extensive work in our laboratory showed that such films have an 80% failure rate when wet. Using the principle of differential pumping of the microscope column, we can use open apertures in place of thin film windows.Fig. 1 shows the modified Siemens la specimen chamber with the connections to the water supply and the auxiliary pumping station. A mechanical pump is connected to the vapor supply via a 100μ aperture to maintain steady-state conditions.


2021 ◽  
Author(s):  
Wu Lan ◽  
Yuan Peng Du ◽  
Songlan Sun ◽  
Jean Behaghel de Bueren ◽  
Florent Héroguel ◽  
...  

We performed a steady state high-yielding depolymerization of soluble acetal-stabilized lignin in flow, which offered a window into challenges and opportunities that will be faced when continuously processing this feedstock.


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