Effects of hypo- or hyperosmotic stress on gluconeogenesis, phosphoenolpyruvate carboxykinase activity, and gene expression in jaw muscle of the crab Chasmagnathus granulata: seasonal differences

2005 ◽  
Vol 316 (2) ◽  
pp. 203-212 ◽  
Author(s):  
Vanessa Schein ◽  
Ana Lúcia Fernandes Chittó ◽  
Rodrigo Etges ◽  
Luiz Carlos Kucharski ◽  
Alain van Wormhoudt ◽  
...  
Keyword(s):  
Gene Expression ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Hyperosmotic Stress ◽  
Seasonal Differences ◽  
Jaw Muscle ◽  
Chasmagnathus Granulata ◽  
Crab Chasmagnathus Granulata

Antioxidant Defenses and DNA Damage Induced by UV-A and UV-B Radiation in the Crab Chasmagnathus granulata (Decapoda, Brachyura)¶

2005 ◽  
Vol 81 (2) ◽  
pp. 398 ◽  
Author(s):  
Glauce R. Gouveia ◽  
Daiane S. Marques ◽  
Bruno P. Cruz ◽  
Laura A. Geracitano ◽  
Luiz E. M. Nery ◽  
...  
Keyword(s):  
Dna Damage ◽  
Antioxidant Defenses ◽  
Chasmagnathus Granulata ◽  
Crab Chasmagnathus Granulata

scholarly journals Developmentally regulated interactions of liver nuclear factors with the rat phosphoenolpyruvate carboxykinase promoter.

1990 ◽  
Vol 10 (5) ◽  
pp. 2418-2422 ◽  
Author(s):  
M Trus ◽  
N Benvenisty ◽  
H Cohen ◽  
L Reshef
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Fetal Liver ◽  
Sequential Pattern ◽  
Late Development ◽  
Developmentally Regulated ◽  
Nuclear Factors

A sequential pattern of interactions of trans-acting factors in rat liver with the phosphoenolpyruvate carboxykinase promoter during late development was observed. A liver-enriched factor, possibly AF1, interacted with the promoter in fetal liver, whereas a factor with the characteristics of C/EBP bound the promoter after birth with the onset of the gene expression.

trans activation of rat phosphoenolpyruvate carboxykinase (GTP) gene expression by micro-coinjection of rat liver mRNA in Xenopus laevis oocytes

1989 ◽  
Vol 9 (11) ◽  
pp. 5244-5247
Author(s):  
N Benvenisty ◽  
T Shoshani ◽  
Y Farkash ◽  
H Soreq ◽  
L Reshef
Keyword(s):  
Gene Expression ◽  
Xenopus Laevis ◽  
Reporter Gene ◽  
Rat Liver ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Xenopus Laevis Oocytes ◽  
Activation Process ◽  
Tissue Specific ◽  
Chimeric Construct

To study the liver-specific trans activation of the rat phosphoenolpyruvate carboxykinase (PEPCK) gene, the PEPCK promoter was linked to a reporter gene and was microinjected into Xenopus laevis oocytes alone or in conjunction with rat liver poly(A)+ RNA. The rat liver mRNA markedly enhanced the expression of the PEPCK-chimeric construct. This effect appeared to be sequence specific, as it was dependent on the presence of the intact promoter. Moreover, the RNA effect was limited to mRNA preparations from PEPCK-expressing tissues only. Finally, microinjection of size-fractionated liver mRNA revealed that the trans-acting factor(s) is encoded by RNA of 1,600 to 2,000 nucleotides, providing a direct bioassay for the gene(s) involved in this tissue-specific trans-activation process.

scholarly journals Anticholinesterase effect of eserine (physostigmine) in fish and crustacean species

2001 ◽  
Vol 44 (1) ◽  
pp. 63-68 ◽  
Author(s):  
José M. Monserrat ◽  
Adalto Bianchini
Keyword(s):  
Cholinesterase Activity ◽  
Fish Larvae ◽  
Kinetic Characteristic ◽  
Natural Substrate ◽  
Crustacean Species ◽  
Lower Affinity ◽  
Substrate Analogues ◽  
Farfantepenaeus Paulensis ◽  
Chasmagnathus Granulata ◽  
Crab Chasmagnathus Granulata

The kinetic characteristic (Km) of cholinesterase from the crab Chasmagnathus granulata, the shrimp Farfantepenaeus paulensis and the fish Odontesthes bonaeriensis were compared and correlated with the anticholinesterasic effect of eserine (physostigmine). For the crustaceans, the estimated Km values were about 5-8 times higher than that estimated for the fish (0.04 mM). In the crab and the shrimp, the concentration of eserine which inhibited 50% of cholinesterase activity (IC50) was estimated as 5.33x10-4 and 4.33x10-4 mM, respectively. In both cases, it was significantly higher (P < 0.05) than that estimated for the fish larvae (7.43x10-5 mM). A high Km could reflect a lower affinity of the cholinesterase for its natural substrate, acetylcholine, or for substrate analogues such as carbamates and organophosphorous pesticides. If we consider the IC50 for eserine as an index of enzyme susceptibility to pesticide inhibition, the cholinesterase from the fish larvae may be a better useful tool in assays for pesticide biomonitoring than that from crustacean species.

Hormonal Regulation of Phosphoenolpyruvate Carboxykinase Gene Expression

1985 ◽  
pp. 347-368 ◽  
Author(s):  
David S. Loose ◽  
Anthony Wynshaw-Boris ◽  
Herman M. Meisner ◽  
Yaacov Hod ◽  
Richard W. Hanson
Keyword(s):  
Gene Expression ◽  
Hormonal Regulation ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Phosphoenolpyruvate Carboxykinase Gene
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