Cytotoxicity and antimitotic activity of Rhinella schneideri and Rhinella marina venoms

Maytansine, a novel ansa macrolide (1), has potent anti-tumor and antimitotic activity (2, 3). It blocks cell cycle traverse in mitosis with resultant accumulation of metaphase cells (4). Inhibition of brain tubulin polymerization in vitro by maytansine has also been reported (3). The C-mitotic effect of this drug is similar to that of the well known Vinca- alkaloids, vinblastine and vincristine. This study was carried out to examine the effects of maytansine on the cell cycle traverse and the fine struc- I ture of human lymphoblasts.Log-phase cultures of CCRF-CEM human lymphoblasts were exposed to maytansine concentrations from 10-6 M to 10-10 M for 18 hrs. Aliquots of cells were removed for cell cycle analysis by flow microfluorometry (FMF) (5) and also processed for transmission electron microscopy (TEM). FMF analysis of cells treated with 10-8 M maytansine showed a reduction in the number of G1 cells and a corresponding build-up of cells with G2/M DNA content.

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Uultrastructure of Microtubules

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094528 ◽

1972 ◽

Vol 30 ◽

pp. 252-253

Author(s):

Ariaki Nagayama

Keyword(s):

Fine Structure ◽

Cultured Cells ◽

Present Report ◽

Confluent Monolayer ◽

Purification Method ◽

Vinca Rosea ◽

L Cells ◽

Antimitotic Activity ◽

Monolayer Cultures ◽

Rapid Purification

Vinblastine(Vb) or vincristine, alkaloid derived from Vinca rosea is known for its antimitotic activity by regrouping of microtubules into paracrystalline form within the cells. A rapid purification method of vinblastine-induced microtubular paracrystals(PC) has provided us with a fresh and pure microtubular material demonstrating the presence of a labile ATPase associated with the PC. The present report is concerned with the fine structure of purified microtubules of mammalian cultured cells.Confluent monolayer cultures of L cells were incubated for 20hrs with 10-5 M Vb (donated from Shionogi Seiyaku & Co., Osaka, Japan).

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Antimitotic Activity on Sea Urchin Embryonic Cells of Seven Antiparasitic Morita-Baylis-Hillman Adducts: A Potential New Class of Anticancer Drugs

Medicinal Chemistry ◽

10.2174/1573406411208061003 ◽

2012 ◽

Vol 8 (6) ◽

pp. 1003-1011

Author(s):

Jocelmo C. A. Leite ◽

Claudio G. L. Junior ◽

Fabio P. L. Silva ◽

Suervy C.O. Sousa ◽

Mario L. A. A. Vasconcellos ◽

...

Keyword(s):

Anticancer Drugs ◽

Sea Urchin ◽

Embryonic Cells ◽

New Class ◽

Antimitotic Activity

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Cytotoxicity potential of chemical constituents isolated and derivatised from Rhinella marina venom

Toxicon ◽

10.1016/j.toxicon.2021.02.006 ◽

2021 ◽

Vol 194 ◽

pp. 37-43

Author(s):

Evaldo dos Santos Monção Filho ◽

Mariana Helena Chaves ◽

Paulo Michel Pinheiro Ferreira ◽

Cláudia Pessoa ◽

Daisy Jereissati Barbosa Lima ◽

...

Keyword(s):

Chemical Constituents ◽

Rhinella Marina

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Giant Toads (Rhinella marina) From the Industrial Zones of Low Basin of the Coatzacoalcos River (Veracruz, MX) Presents Genotoxicity in Erythrocytes

Bulletin of Environmental Contamination and Toxicology ◽

10.1007/s00128-021-03162-2 ◽

2021 ◽

Author(s):

Omar Cruz-Santiago ◽

Claudia G. Castillo ◽

Guillermo Espinosa-Reyes ◽

Iván N. Pérez-Maldonado ◽

Donaji J. González-Mille ◽

...

Keyword(s):

Rhinella Marina ◽

Industrial Zones

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An invasion in slow motion: the spread of invasive cane toads (Rhinella marina) into cooler climates in southern Australia

Biological Invasions ◽

10.1007/s10530-021-02597-2 ◽

2021 ◽

Author(s):

Lincoln F. Macgregor ◽

Matthew Greenlees ◽

Mark de Bruyn ◽

Richard Shine

Keyword(s):

Slow Motion ◽

Rhinella Marina

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A large new species of Rhinella (Anura: Bufonidae) from Cerrado of Brazil

Zootaxa ◽

10.11646/zootaxa.1627.1.2 ◽

2007 ◽

Vol 1627 (1) ◽

pp. 23-39 ◽

Cited By ~ 11

Author(s):

NATAN M. MACIEL ◽

REUBER A. BRANDÃO ◽

LEANDRO A. CAMPOS ◽

ANTONIO SEBBEN

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Morphological Characters ◽

Morphological Features ◽

Advertisement Call ◽

Sexually Dimorphic ◽

Rhinella Marina ◽

Large Size ◽

Morphometric Feature ◽

Distrito Federal

A new toad, Rhinella cerradensis, is described, including its tadpole and the advertisement call. The new species occupies Cerrado habitats in the Brazilian states of Piauí, Bahia, Goiás, Minas Gerais, and Distrito Federal. The species is characterized by its large size; absence of tibial glands; well developed cranial crests; short hands; sexually dimorphic coloration; and by the absence of a spiracle tube of the tadpole. The new species is included in the Rhinella marina group by the presence of a jagged suture formed by the articulation between the pterygoid medial ramus and the parasphenoid alae, as well as other shared morphological features. Morphological characters and statistical analyses inferred by morphometric feature suggest the existence of two subgroups of species within R. marina group. However, taxonomic rearrangements are not made here and await phylogenetic analysis.

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THE MEDIATOR OF CELLULAR IMMUNITY

Journal of Experimental Medicine ◽

10.1084/jem.137.3.660 ◽

1973 ◽

Vol 137 (3) ◽

pp. 660-674 ◽

Cited By ~ 18

Author(s):

D. D. McGregor ◽

Pamela S. Logie

Keyword(s):

Host Reaction ◽

Proliferating Cells ◽

Cellular Resistance ◽

Effector Cells ◽

Profound Impact ◽

Antimitotic Activity ◽

Antimitotic Drug ◽

Autoradiographic Analysis ◽

Central Lymph ◽

Listeria Infection

The antimitotic drug vinblastine (Vbl) has a profound impact upon the specifically sensitized lymphocytes that transfer cellular resistance to Listeria monocytogenes. A 12-h pulse of the drug given to prospective donors during the first week of an immunizing Listeria infection inhibits the delivery of protective lymphocytes to the thoracic duct and their subsequent movement into an inflammatory exudate induced in the peritoneal cavity. The effect of Vbl is clearly related to its antimitotic activity, not to an effect on lymphocytes regardless of their position in the division cycle. This conclusion was drawn from an autoradiographic analysis of cells in the lymph of Vbl-treated rats and from failure of the drug to abrogate a known function of small lymphocytes, namely, their ability to initiate a graft-vs.-host reaction. The results imply that large lymphocytes, the rapidly proliferating cells in central lymph, are the principal effector cells responsible for transmitting resistance to L. monocytogenes and provide a plausible explanation for their rapid turnover and short circulating life-span.

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