scholarly journals Anti-inflammatory activity of the water extract of Chloranthus serratus roots in LPS-stimulated RAW264.7 cells mediated by the Nrf2/HO-1, MAPK and NF-κB signaling pathways

2021 ◽  
Vol 271 ◽  
pp. 113880
Author(s):  
Shuping Sun ◽  
Jiahao Zhang ◽  
Hongxing Li ◽  
Yunyan Du ◽  
Shengli Li ◽  
...  
Keyword(s):  
Signaling Pathways ◽  
Water Extract ◽  
Inflammatory Activity ◽  
Raw264.7 Cells ◽  
Anti Inflammatory

scholarly journals Anti-Inflammatory Activity of the Phlorotannin Trifuhalol A Using LPS-Stimulated RAW264.7 Cells Through NF-κB and MAPK Main Signaling Pathways

2019 ◽  
Vol 14 (5) ◽  
pp. 1934578X1984979 ◽  
Author(s):  
Kasira Phasanasophon ◽  
Sang Moo Kim
Keyword(s):  
Nitric Oxide ◽  
Ethyl Acetate ◽  
Signaling Pathways ◽  
Ethyl Acetate Fraction ◽  
Inflammatory Activity ◽  
Raw264.7 Cells ◽  
No Production ◽  
Mitogen Activated Protein ◽  
Anti Inflammatory ◽  
Factor Α

Trifuhalol A, a phlorotannin, was extracted from Agarum cribrosum with ethyl acetate and fractionated using Sephadex LH-20 column chromatography (SF1-SF6). The ethyl acetate fraction (EAF) and SF5-containing trifuhalol A exhibited strong inhibitory activity against hyaluronidase. The anti-inflammatory activity of the phlorotannin, EAF, and SF5 was determined through the inhibition of nitric oxide (NO) production in lipopolysaccharide-stimulated RAW264.7 cells. Furthermore, the inhibition of NO production was validated by confirming the appreciable downregulation of inducible nitric oxide synthase expression. Agarum cribrosum phlorotannin also markedly suppressed the expression of cyclooxygenase-2, interleukin (IL)-1β, IL-6, and tumor necrosis factor-α. In addition, the anti-inflammatory action was verified by examining its effects on proinflammatory signaling pathways. The activation of nuclear factor (NF)-κB and mitogen-activated protein kinases (MAPKs) was attenuated via the inhibition of NF-κB p-65, c-Jun N-terminal kinase, extracellular signal-regulated kinase 1/2, and p38 MAPK phosphorylation. Therefore, trifuhalol A is a potential source for either the prevention or the treatment of inflammation.

scholarly journals Scientific Evaluation of anti-inflammatory potential of Macrotyloma uniflorum seeds extracts

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1883-1888
Author(s):  
Anandarajagopal K ◽  
Abdullah Khan ◽  
Sugalia S ◽  
Bama Menon ◽  
Tan Ching Siang ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Water Extract ◽  
Ethanol Extract ◽  
Inflammatory Activity ◽  
Leguminous Plant ◽  
Paw Edema ◽  
Reference Drug ◽  
Macrotyloma Uniflorum ◽  
Phenolic Constituents ◽  
Anti Inflammatory

Phytochemicals possessing the antioxidant properties naturally present in food attract a greater interest to healthcare researchers due to their desirable health effects on human health as they can be explored for protection against oxidative deterioration. Macrtotyloma uniflorum is a leguminous plant belonging to the family Fabaceae and commonly known as Horse gram. Aqueous and ethanol extracts of seeds of Macrotyloma uniflorum were evaluated for their anti-inflammatory effects using the scientific protocol on experimental rats. Extraction was carried out using the cold maceration method, and the anti-inflammatory activity was evaluated using a digital plethysmometer in the experimental rats injected with carrageenan to produce paw edema. Preliminary phytochemical studies confirmed the presence of various bioactive compounds such as alkaloids, glycosides, carbohydrates, proteins and amino acids, terpenoids, tannins, and phenolic compounds in both extracts while flavonoids were found only in ethanol extract. Both extracts of M. uniflorum seeds (200 mg/ml) significantly (p<0.01) reduced the paw edema volume induced by carrageenan. The ethanol extract of M. uniflorum seeds exhibited more potent anti-inflammatory activity than water extract, that might be due to the presence of flavonoids in ethanol extract. The activity of the extracts was compared with diclofenac sodium (10mg/kg b.wt.) as a reference drug. From the results, it may be suggested that the antioxidative potential of phenolic constituents and flavonoids is the primary factors for the anti-inflammatory activity of M. uniflorum seeds extracts.

Archidendron lucidum Exerts Anti-Inflammatory Effects by Targeting PDK1 in the NF-κB Pathway

2020 ◽  
Vol 48 (02) ◽  
pp. 429-444
Author(s):  
Minkyeong Jo ◽  
Young-Su Yi ◽  
Jae Youl Cho
Keyword(s):  
Signaling Pathway ◽  
Hplc Analysis ◽  
Inflammatory Diseases ◽  
Interleukin 1 ◽  
Inflammatory Activity ◽  
Raw264.7 Cells ◽  
Luciferase Reporter ◽  
No Production ◽  
Pharmacological Activities ◽  
Anti Inflammatory

Pharmacological activities of some Leguminosae family members were reported. Pharmacological activities of Archidendron lucidum, a Leguminosae family member have never been explored. Therefore, this study investigated anti-inflammatory effects of an Archidendron lucidum methanol extract (Al-ME). In this study, anti-inflammatory effects of Al-ME were investigated in LPS-stimulated RAW264.7 cells and HCl/EtOH-induced gastritis mice by MTT assay, nitric oxide (NO) production assay, semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR), luciferase reporter assay, and Western blotting. High-performance liquid chromatography (HPLC) analysis identified ethnopharmacological compounds in Al-ME. Al-ME inhibited NO production without cytotoxicity in peritoneal macrophages and RAW264.7 cells stimulated with LPS or Pam3CSK4. Al-ME downregulated mRNA expression of inflammatory genes (inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2)) and pro-inflammatory cytokines (tumor necrosis factor-[Formula: see text] (TNF-[Formula: see text]), interleukin-1[Formula: see text] (IL-1[Formula: see text]), and IL-6). Al-ME exerted anti-inflammatory activity in LPS-stimulated RAW264.7 cells by inhibiting nuclear factor-kappa B (NF-[Formula: see text]B) signaling pathway. HPLC analysis identified quercetin, luteolin, and kaempferol as major anti-inflammatory components in Al-ME. Al-ME ameliorated HCl/EtOH-induced gastritis symptoms in mice by suppressing iNOS and IL-6 mRNA expressions and I[Formula: see text]B[Formula: see text] phosphorylation. Therefore, these results suggest that Al-ME exhibited anti-inflammatory activity by targeting NF-[Formula: see text]B signaling pathway, implying that Al-ME could be potent anti-inflammatory medications to prevent and treat inflammatory diseases.

Sageretia thea Inhibits Inflammation through Suppression of NF-κB and MAPK and Activation of Nrf2/HO-1 Signaling Pathways in RAW264.7 Cells

2019 ◽  
Vol 47 (02) ◽  
pp. 385-403 ◽  
Author(s):  
Ha Na Kim ◽  
Gwang Hun Park ◽  
Su Bin Park ◽  
Jeong Dong Kim ◽  
Hyun Ji Eo ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Mapk Signaling ◽  
Inflammatory Activity ◽  
Nuclear Accumulation ◽  
Raw264.7 Cells ◽  
No Production ◽  
Immunodeficiency Virus ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Signaling Activation

Sageretia thea (S. thea) commonly known as Chinese sweet plum or Chinese bird plum has been used for treating hepatitis and fevers in Korea and China. S. thea has been reported to exert anti-oxidant, anticancer and anti-human immunodeficiency virus activity. However, there is little study on the anti-inflammatory activity of S. thea. Thus, we evaluated the anti-inflammatory effect of extracts of leaves (ST-L) and branches (ST-B) from Sageretia thea in LPS-stimulated RAW264.7 cells. ST-L and ST-B significantly inhibited the production of the pro-inflammatory mediators such as NO, iNOS, COX-2, IL-1[Formula: see text] and IL-6 in LPS-stimulated RAW264.7 cells. ST-L and ST-B blocked LPS-induced degradation of I[Formula: see text]B-[Formula: see text] and nuclear accumulation of p65, which resulted in the inhibition of NF-[Formula: see text]B activation in RAW264.7 cells. ST-L and ST-B also attenuated the phosphorylation of ERK1/2, p38 and JNK in LPS-stimulated RAW264.7 cells. In addition, ST-L and ST-B increased HO-1 expression in RAW264.7 cells, and the inhibition of HO-1 by ZnPP reduced the inhibitory effect of ST-L and ST-B against LPS-induced NO production in RAW264.7 cells. Inhibition of p38 activation and ROS elimination attenuated HO-1 expression by ST-L and ST-B, and ROS elimination inhibited p38 activation induced by ST-L and ST-B. ST-L and ST-B dramatically induced nuclear accumulation of Nrf2, but this was significantly reversed by the inhibition of p38 activation and ROS elimination. Collectively, our results suggest that ST-L and ST-B exerts potential anti-inflammatory activity by suppressing NF-[Formula: see text]B and MAPK signaling activation, and activating HO-1 expression through the nuclear accumulation of Nrf2 via ROS-dependent p38 activation. These findings suggest that ST-L and ST-B may have great potential for the development of anti-inflammatory drug to treat acute and chronic inflammatory disorders.

scholarly journals Anti-inflammatory Activity of Dichloromethane Extract of Auricularia auricula-judae in RAW264.7 Cells

2011 ◽  
Vol 27 (1) ◽  
pp. 11-14 ◽  
Author(s):  
Dereje Damte ◽  
Md. Ahsanur Reza ◽  
Seung-Jin Lee ◽  
Woo-Sik Jo ◽  
Seung-Chun Park
Keyword(s):  
Inflammatory Activity ◽  
Raw264.7 Cells ◽  
Auricularia Auricula ◽  
Anti Inflammatory
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