Oleocanthalic acid from extra-virgin olive oil: analysis, preparative isolation and radical scavenging activity

Author(s):  
Jasmine Esposito Salsano ◽  
Diana Pinto ◽  
Francisca Rodrigues ◽  
Alessandro Saba ◽  
Clementina Manera ◽  
...  
2013 ◽  
Vol 781-784 ◽  
pp. 1776-1779
Author(s):  
Li Qin Jiang

The aim of this study was to investigate the effect of antioxidants of soybean oil and virgin olive oil during heating. Tocopherol and total phenol content in oils were determined by HPLC and Folin test, respectively. Antioxidant activity was measured with scavenging-activity (RSA) of free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH·) by spectrophotometer. Among the antioxidants studied, comparable activity was found between Trolox and tocopherol, while activity of phenol was low in the study. Tocopherol compounds contribute the RSA of soybean oil, and the RSA of virgin olive oil was from phenol compound as well as tocopherols. Our study showed the variety and content of antioxidants influence RSA of vegetable oils, which concerned the stability and nutrition quality of vegetable oil.


Author(s):  
M. Leporini, M. R. Loizzo, M. C. Tenuta, T. Falco ◽  
Vincenzo Sicari, Teresa M. Pellicano ◽  
Rosa Tundis

Extra virgin olive oil (EVOO) plays a crucial role in the Mediterranean diet. Recently, attention has been focused on presence in EVOO of phenolic compounds, phytochemicals characterized by a series of healthy properties. This paper analyzed the phenolic profile, the inhibitory activity against carbohydrate hydrolising enzyme as well as the radical scavenging activity of EVOO obtained from Olea europea L. cv. Frantoio. Samples derived from fruits collected in four different areas: Cariati, Vaccarizzo Albanese, Montalto Uffugo, and Praia a Mare. The phenolic profile obtained by HPLC revealed the presence of hydroxytyrosol (3,4-DHPEA, between 1.2 and 5.3 mg/kg) and p-hydroxyphenylethanol or tyrosol (p-HPEA, between 1.1 and 5.4 mg/kg), as the main components. Secoiridoids and their derivatives were also found in high concentrations (3,4-DHPEA-EDA 50.3-98.4 mg/kg, p-HPEA-EDA 34.6-52.9 mg/kg). All samples showed carbohydrate-hydrolyzing enzymes inhibition. The most promising activity was observed with EVOO from Vaccarizzo Albanese (IC50 of 65.5 and 57.7 µg/ml against α-glucosidase and α-amylase, respectively). The same sample showed the highest antioxidant activity of 45.3 and 56.3 µg/ml against DPPH and ABTS radicals, respectively. This sample was richest in phenols. In conclusion, EVOO has high-level bioactive compounds and a promising antioxidant and hypoglycemic activity.


Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 996
Author(s):  
Vito Michele Paradiso ◽  
Federica Flamminii ◽  
Paola Pittia ◽  
Francesco Caponio ◽  
Carla Di Mattia

Omics approaches are recently being applied also in food lipid oxidation, to increase knowledge of oxidation and antioxidation mechanisms. The so-called oxidomics throws a wider spot of light on the complex patterns of reactions taking place in food lipids, especially in dispersed systems. This research aimed to investigate the radical scavenging activity of olive oil phenolic antioxidants (OPAs) in O/W emulsions, as affected by the phase in which they were added. This allowed one to assess whether different behaviors could be expected from antioxidants originally present in phenolic-rich olive oils compared to natural antioxidants added in the water phase during emulsion production. Hydroperoxide decomposition kinetics and the analysis of volatile pattern provided an outline of antioxidation mechanisms. Though being effective in slowing down oxidation when added both in the oil and water phase, OPAs interfered in different ways with oxidation pathways, based on the phase in which they were added. OPAs added to the water phase were more effective in slowing down hydroperoxide decomposition due to the hydrophilic radical initiator. On the other hand, OPAs present in the oil were more effective in preventing radical propagation, with relevant consequences on the volatile pattern.


Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 470 ◽  
Author(s):  
Abril ◽  
Mirabal-Gallardo ◽  
González ◽  
Marican ◽  
Durán-Lara ◽  
...  

We investigated the potential of two oil extracts from seeds of Colliguaya integerrima (CIO) and Cynara cardunculus (CO) to use as nutritionally edible oils. For this purpose, oil quality was accessed by determining the fatty acid composition, peroxide value, acid value, iodine value, saponification number, phenolic contents, and oxidative stability during thermally induced oxidation of CIO and CO oils and compared to those of extra-virgin olive oil (EVOO). The chemical composition results demonstrated that both oils could be nutritional sources of essential unsaturated fatty acids. Moreover, according to the gravimetric analysis, the main decomposition step occurred in the temperature range of 200–420 °C, showing a similar thermal behavior of EVOO oil. However, CO and EVOO oils showed a higher phenolic content at degradation onset temperature (T0) in contrast with CIO oil. The antioxidant activity of the different studied oils showed a direct correlation with the phenol contents, up to temperatures around 180 °C, where the percentage of free radical scavenging assay for EVOO was higher than CO in contrast with the TPC values. Finally, we analyzed the minor components before and after heating CIO and CO at 180 °C by gas chromatography–mass spectrometry (GC–MS) using library search programs.


Foods ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 610 ◽  
Author(s):  
Vasiliki Skiada ◽  
Panagiotis Tsarouhas ◽  
Theodoros Varzakas

While there has been considerable research related to Koroneiki cultivar in different areas in Greece, no systematic work has been carried out on olive oil analysis from one of the most important olive-growing regions in Greece, located southwest of Peloponnese, Messinia. This work is the first systematic attempt to study the profile of Messinian olive oils and evaluate to what extent they comply with the recent EU regulations in order to be classified as “Kalamata Protected Designation of Origin (PDO)”-certified products. Quality indices were measured and detailed analyses of sterols, triterpenic dialcohols, fatty acid composition and wax content were conducted in a total of 71 samples. Messinian olive oils revealed a high-quality profile but, at the same time, results demonstrated major fluctuations from the established EU regulatory limits on their chemical parameters. Results showed low concentrations of total sterols, with 66.7% of the examined samples below the regulated set limits for Kalamata PDO status; high concentrations of campesterol, with a total of 21.7%, exceeding the legal maximum of 4.0%; and a slight tendency of high total erythrodiol content. Fatty acid composition and wax content were within the normal range expected for the extra virgin olive oil (EVOO) category. However, the narrower established PDO limits in specific fatty acids showed some fluctuations in a few cases.


Author(s):  
Aadil Bajoub ◽  
Alegría Carrasco-Pancorbo ◽  
Noureddine Ouazzani ◽  
Alberto Fernández-Gutiérrez

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