Clinicopathological characteristics and prognosis in significantly thick acral lentiginous melanoma in Taiwan

This study aimed to establish the clinicopathological characteristics of patients with melanoma and its association with BRAF gene mutations. The pathology reports and paraffin-embedded tumor samples from 47 women and 30 men with melanoma, with an average age of diagnosis of 60 years, were reviewed at the Hospital Federico Lleras Acosta of Ibague, between 2010 and 2016. The presence of V600E mutation at the exon 15 of BRAF gene, was analyzed in these tumoral samples by Sanger sequencing and visual inspection of the electropherograms. We also studied the clinicopathological variables with X2, t-Student and the Kaplan Meier index. Most of the lesions were located in the lower limbs (46.6%). The most frequent subtype was Acral Lentiginous Melanoma (41.8%). Most lesions were of poor prognosis: Breslow depth greater than 4.1 mm (52.7%), ulceration (61.4%) and medium or high mitotic rate (> 30 %). The V600E mutation was identified in five patients with large, deep and ulcerated tumors, four of them had less than four years of survival. In conclusion, there was a higher frequency of melanoma in women, V600E BRAF mutation was present in patients with advanced disease (high Breslow index) and, the probability of five-year survival was less than 40%.

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Acral lentiginous melanoma: clinicopathological characteristics and survival outcomes in the US National Cancer Database 2004–2016

British Journal of Dermatology ◽

10.1111/bjd.19211 ◽

2020 ◽

Vol 183 (5) ◽

pp. 952-954

Author(s):

S. Behbahani ◽

S. Malerba ◽

F.H. Samie

Keyword(s):

Clinicopathological Characteristics ◽

Survival Outcomes ◽

National Cancer Database ◽

Acral Lentiginous Melanoma ◽

The Us

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Mutations in the BRAF, NRAS, and C-KIT Genes of Patients Diagnosed with Melanoma in Colombia Population

BioMed Research International ◽

10.1155/2020/2046947 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Luz D. Gutiérrez-Castañeda ◽

Mauricio Gamboa ◽

John A. Nova ◽

Leonardo Pulido ◽

Jose D. Tovar-Parra

Keyword(s):

Clinicopathological Characteristics ◽

Geographic Region ◽

Lentigo Maligna ◽

Histological Subtypes ◽

Braf Gene ◽

Tissue Samples ◽

Acral Lentiginous Melanoma ◽

Kit Gene ◽

Lentigo Maligna Melanoma ◽

Sanger Method

Introduction. Mutations in the BRAF, NRAS, and C-KIT genes have been associated with the histopathological characteristics of melanoma. Likewise, the incidence of each of these subtypes changes according to the geographical origin of the population analyzed. Objective. To determine the mutation frequency in exons 11 and 15 of the BRAF gene, exons 1 and 2 of the NRAS gene, and exons 11, 13, and 17 of the C-KIT gene and to relate it with histological subtypes in patients from a region with high levels of ultraviolet radiation. Methodology. The clinicopathological characteristics of 54 cutaneous melanoma samples were analyzed. Mutation analysis was performed via qPCR on paraffin-embedded tumor tissue samples using probes specific for the V600E mutation. Amplification of exons 11 and 15 of the BRAF gene, exons 1 and 2 of the NRAS gene, and exons 11, 13, and 17 of the C-KIT gene was performed for subsequent sequencing using the Sanger method. Result. The most frequent histological subtype in the analyzed sample was lentigo maligna/lentigo maligna melanoma (52%) followed by acral lentiginous melanoma (20%). The BRAF-V600 variant was the most frequent (63.6%). The most frequent (54%) mutation in NRAS was p.Lys5∗. In the C-KIT gene, only the Val560Ala mutation was found. Conclusion. Differences in histological subtypes and mutations in the BRAF, NRAS, and C-KIT genes were found in the analyzed population. This indicates that environmental and genetic factors significantly influence the introduction of the disease in this geographic region.

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Melanoma in Singapore: A 20-year review of disease and treatment outcomes

Annals of the Academy of Medicine, Singapore ◽

10.47102/annals-acadmedsg.2020535 ◽

2021 ◽

Vol 50 (6) ◽

pp. 456-466 ◽

Cited By ~ 1

Author(s):

Pei Ming Yeo ◽

Ziying Vanessa Lim ◽

Wei Ding Virlynn Tan ◽

Xiahong Zhao ◽

Hui Yi Chia ◽

...

Keyword(s):

Treatment Outcomes ◽

Cutaneous Melanoma ◽

Mortality Rates ◽

Clinicopathological Characteristics ◽

Time Interval ◽

Cancer Awareness ◽

Tertiary Referral ◽

Superficial Spreading ◽

Acral Lentiginous Melanoma ◽

Median Time Interval

Introduction: Melanomas in Asians have different clinicopathological characteristics and prognosis from melanomas in Caucasians. This study reviewed the epidemiology and treatment outcomes of cutaneous melanoma diagnosed at a tertiary referral dermatology centre in Singapore, which has a multiracial population. The study also determined whether Asians had comparable relapse-free and overall survival periods to Caucasians in Singapore. Method: This is a retrospective review of cutaneous melanoma cases in our centre between 1996 and 2015. Results: Sixty-two cases of melanoma were diagnosed in 61 patients: 72.6% occurred in Chinese, 19.4% in Caucasians and 3.2% in Indians, with an over-representation of Caucasians. Superficial spreading melanoma, acral lentiginous melanoma and nodular melanoma comprised 37.1%, 35.5% and 22.6% of the cases, respectively. The median time interval to diagnosis was longer in Asians than Caucasians; median Breslow’s thickness in Asians were significantly thicker than in Caucasians (2.6mm versus 0.9mm, P=0.018) and Asians tend to present at a later stage. The mortality rates for Asians and Caucasians were 52% and 0%, respectively. Conclusion: More physician and patient education on skin cancer awareness is needed in our Asian-predominant population for better outcomes. Keywords: Asian, melanoma, nails, skin neoplasm, survival

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