Molecular mechanism of catalase activity change under sodium dodecyl sulfate-induced oxidative stress in the mouse primary hepatocytes†

2016 ◽  
Vol 307 ◽  
pp. 173-183 ◽  
Author(s):  
Jing Wang ◽  
Jiaxi Wang ◽  
Chi Xu ◽  
Rutao Liu ◽  
Yadong Chen
Keyword(s):  
Oxidative Stress ◽  
Sodium Dodecyl Sulfate ◽  
Molecular Mechanism ◽  
Catalase Activity ◽  
Sodium Dodecyl ◽  
Activity Change ◽  
Primary Hepatocytes ◽  
Dodecyl Sulfate

scholarly journals Optimizing Perfusion-Decellularization Methods of Porcine Livers for Clinical-Scale Whole-Organ Bioengineering

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Qiong Wu ◽  
Ji Bao ◽  
Yong-jie Zhou ◽  
Yu-jia Wang ◽  
Zheng-gui Du ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Sodium Dodecyl Sulfate ◽  
Liver Tissue ◽  
Sodium Dodecyl ◽  
Sodium Deoxycholate ◽  
Great Promise ◽  
Primary Hepatocytes ◽  
Porcine Liver ◽  
Liver Tissue Engineering ◽  
Dodecyl Sulfate

Aim. To refine the decellularization protocol of whole porcine liver, which holds great promise for liver tissue engineering.Methods. Three decellularization methods for porcine livers (1% sodium dodecyl sulfate (SDS), 1% Triton X-100 + 1% sodium dodecyl sulfate, and 1% sodium deoxycholate + 1% sodium dodecyl sulfate) were studied. The obtained liver scaffolds were processed for histology, residual cellular content analysis, and extracellular matrix (ECM) components evaluation to investigate decellularization efficiency and ECM preservation. Rat primary hepatocytes were seeded into three kinds of scaffold to detect the biocompatibility.Results. The whole liver decellularization was successfully achieved following all three kinds of treatment. SDS combined with Triton had a high efficacy of cellular removal and caused minimal disruption of essential ECM components; it was also the most biocompatible procedure for primary hepatocytes.Conclusion. We have refined a novel, standardized, time-efficient, and reproducible protocol for the decellularization of whole liver which can be further adapted to liver tissue engineering.

Unraveling the molecular mechanism of the effects of sodium dodecyl sulfate, salts, and sugars on amyloid fibril formation in camel IgG

2018 ◽  
Vol 170 ◽  
pp. 430-437 ◽  
Author(s):  
Mohamad Alhasan Ismael ◽  
Javed Masood Khan ◽  
Ajamaluddin Malik ◽  
Mohammad A. Alsenaidy ◽  
Syed Hidayathulla ◽  
...  
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Molecular Mechanism ◽  
Amyloid Fibril ◽  
Sodium Dodecyl ◽  
Fibril Formation ◽  
Amyloid Fibril Formation ◽  
Dodecyl Sulfate ◽  
Sulfate Salts
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