Characterization of a Novel Plasmid in Serratia marcescens Harbouring blaGES-5 Isolated from a Nosocomial Outbreak in Japan

Characterization of a Novel Plasmid in Serratia marcescens Harboring blaGES-5 Isolated from a Nosocomial Outbreak in Japan

10.1101/2021.07.27.454091 ◽

2021 ◽

Author(s):

Noriko Nakanishi ◽

Shoko Komatsu ◽

Tomotada Iwamoto ◽

Ryohei Nomoto

Keyword(s):

Serratia Marcescens ◽

Treatment Options ◽

Carbapenem Resistance ◽

Clinical Settings ◽

Nosocomial Outbreak ◽

Nosocomial Pathogen ◽

Surveillance Programs ◽

Comparative Genetic Analysis ◽

Unique Plasmid

Serratia marcescens is a nosocomial pathogen with carbapenem resistance, limiting the availability of effective treatment options. In this study, we performed molecular characterization of GES-5 carbapenemase-producing S. marcescens isolated from an outbreak in Japan. Comparative genetic analysis revealed that the blaGES-5–encoding plasmid p2020-O-9 is a unique plasmid contributing towards carbapenem resistance. Furthermore, this study highlights the necessity of surveillance programs for monitoring novel, along with commonly occurring carbapenemases in clinical settings.

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Optimization, purification, and characterization of an extracellular antifungal protein from Serratia marcescens DT3 isolated from soil in Vietnam

TURKISH JOURNAL OF BIOLOGY ◽

10.3906/biy-1607-22 ◽

2017 ◽

Vol 41 ◽

pp. 448-457 ◽

Cited By ~ 1

Author(s):

Tuyen Thi DO ◽

Thao Thi NGUYEN ◽

Thanh Le Sy NGUYEN ◽

Hoang Thanh LE

Keyword(s):

Serratia Marcescens ◽

Antifungal Protein ◽

Purification And Characterization

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Characterization of a Cytotoxic Factor in Culture Filtrates of Serratia marcescens

Infection and Immunity ◽

10.1128/iai.70.3.1121-1128.2002 ◽

2002 ◽

Vol 70 (3) ◽

pp. 1121-1128 ◽

Cited By ~ 32

Author(s):

Kent B. Marty ◽

Christopher L. Williams ◽

Linda J. Guynn ◽

Michael J. Benedik ◽

Steven R. Blanke

Keyword(s):

Cytotoxic Activity ◽

Serratia Marcescens ◽

Mammalian Cells ◽

Divalent Cations ◽

Recombinant Expression ◽

Cytotoxic Factor ◽

Culture Filtrates ◽

Type Strains

ABSTRACT Serratia marcescens culture filtrates have been reported to be cytotoxic to mammalian cells. Using biochemical and genetic approaches, we have identified a major source of this cytotoxic activity. Both heat and protease treatments abrogated the cytotoxicity of S. marcescens culture filtrates towards HeLa cells, suggesting the involvement of one or more protein factors. A screen for in vitro cytotoxic activity revealed that S. marcescens mutant strains that are deficient in production of a 56-kDa metalloprotease are significantly less cytotoxic to mammalian cells. Cytotoxicity was significantly reduced when culture filtrates prepared from wild-type strains were pretreated with either EDTA or 1,10-phenanthroline, which are potent inhibitors of the 56-kDa metalloprotease. Furthermore, cytotoxic activity was restored when the same culture filtrates were incubated with zinc divalent cations, which are essential for enzymatic activity of the 56-kDa metalloprotease. Finally, recombinant expression of the S. marcescens 56-kDa metalloprotease conferred a cytotoxic phenotype on the culture filtrates of a nonpathogenic Escherichia coli strain. Collectively, these data suggest that the 56-kDa metalloprotease contributes significantly to the in vitro cytotoxic activity commonly observed in S. marcescens culture filtrates.

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Gentamicin- and Methicillin-Resistant Staphylococcus aureus: Phenotypic and Genotypic Characterization of Three Putative Nosocomial Outbreak Strains

Chemotherapy ◽

10.1159/000239419 ◽

1996 ◽

Vol 42 (1) ◽

pp. 21-36 ◽

Cited By ~ 4

Author(s):

Walter H. Traub ◽

Birgit Leonhard ◽

Dierk Bauer

Keyword(s):

Staphylococcus Aureus ◽

Nosocomial Outbreak ◽

Methicillin Resistant ◽

Genotypic Characterization ◽

Phenotypic And Genotypic Characterization

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Antimicrobial Resistance and Genomic Characterization of OXA-48- and CTX-M-15-Co-Producing Hypervirulent Klebsiella pneumoniae ST23 Recovered from Nosocomial Outbreak

Antibiotics ◽

10.3390/antibiotics9120862 ◽

2020 ◽

Vol 9 (12) ◽

pp. 862

Author(s):

Elvira Shaidullina ◽

Andrey Shelenkov ◽

Yuri Yanushevich ◽

Yulia Mikhaylova ◽

Dmitriy Shagin ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Illumina Miseq ◽

Type I ◽

Nosocomial Outbreak ◽

Recent Emergence ◽

Genomic Characterization ◽

Global Threat ◽

High Level ◽

Sepsis And Septic Shock

Multidrug resistance (MDR) and hypervirulence (hv) have been long considered distinct evolutionary traits for Klebsiella pneumoniae (Kp), a versatile human pathogen. The recent emergence of Kp strains combining these traits poses a serious global threat. In this article, we describe the phenotypic and genomic characteristics of an MDR hvKp isolate, MAR14-456, representative of a nosocomial outbreak in Moscow, Russia, that was recovered from a postoperative wound in a patient who later developed multiple abscesses, fatal sepsis, and septic shock. Broth microdilution testing revealed decreased susceptibility of MAR14-456 to carbapenems (MICs 0.5–2 mg/L) and a high-level resistance to most β-lactams, β-lactam-β-lactamase-inhibitor combinations, and non-β-lactam antibiotics, except ceftazidime-avibactam, amikacin, tigecycline, and colistin. Whole-genome sequencing using Illumina MiSeq and ONT MinION systems allowed to identify and completely assemble two conjugative resistance plasmids, a typical ‘European’ epidemic IncL/M plasmid that carries the gene of OXA-48 carbapenemase, and an IncFIIK plasmid that carries the gene of CTX-M-15 ESBL and other resistance genes. MLST profile, capsular, lipopolysaccharide, virulence genes encoded on chromosome and IncHI1B/FIB plasmid, and the presence of apparently functional type I-E* CRISPR-Cas system were all characteristic of hvKp ST23, serotype K1-O1v2. Phylogenetic analysis showed the closest relatedness of MAR14-456 to ST23 isolates from China. This report highlights the threat of multiple resistance acquisition by hvKp strain and its spread as a nosocomial pathogen.

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