013 Semaphrin4D drives CD8 + T cells skin trafficking in oral lichen planus via CXCL9 and CXCL10 upregulations in oral keratinocytes

Interleukin- (IL-) 23/IL-17 axis is a newly discovered proinflammatory signaling pathway and has been implicated in the pathogenesis of many chronic inflammatory and immune disorders. Here we investigated whether the IL-23/IL-17 axis was present and functional in the lesions of oral lichen planus (OLP), a chronic inflammatory disease affecting the oral mucosa. Using immunohistochemistry and quantitative PCR, we found that the subunits of IL-23 and IL-17 were overexpressed in OLP lesions than in normal oral mucosa tissues. In addition, the expressions of IL-23 and IL-17 are positively correlated in reticular OLP tissues. Results from in vitro studies revealed that exogenous IL-23 could increase the percentage of Th17 cells and IL-17 production in the CD4+T cells from reticular OLP patients. Furthermore, we also found that exogenous IL-17 could significantly enhance the mRNA expressions ofβ-defensin-2, -3, CCL-20, IL-8, and TNF-α, but notβ-defensin-1, CXCL-9, -10, -11, CCL-5, and IL-6 in human oral keratinocytes. Taken together, our results revealed an overexpression pattern and selectively regulatory roles of IL-23/IL-17 axis in the OLP lesions, suggesting that it may be a pivotal regulatory pathway in the complex immune network of OLP lesions.

Download Full-text

Altered Autophagy-Associated Genes Expression in T Cells of Oral Lichen Planus Correlated with Clinical Features

Mediators of Inflammation ◽

10.1155/2016/4867368 ◽

2016 ◽

Vol 2016 ◽

pp. 1-10 ◽

Cited By ~ 10

Author(s):

Ya-Qin Tan ◽

Jing Zhang ◽

Ge-Fei Du ◽

Rui Lu ◽

Guan-Ying Chen ◽

...

Keyword(s):

T Cells ◽

T Cell ◽

Lichen Planus ◽

Clinical Features ◽

Peripheral Blood ◽

Oral Lichen Planus ◽

T Cell Response ◽

Mrna Levels ◽

Inflammatory Autoimmune Disease ◽

Oral Lichen

Oral lichen planus (OLP) is a T cell-mediated inflammatory autoimmune disease. Autophagy has emerged as a fundamental trafficking event in mediating T cell response, which plays crucial roles in innate and adaptive immunity. The present study mainly investigated the mRNA expression of autophagy-associated genes in peripheral blood T cells of OLP patients and evaluated correlations between their expression and the clinical features of OLP. Five differentially expressed autophagy-associated genes were identified by autophagy array. Quantitative real-time RT-PCR results confirmed thatIGF1expression in the peripheral blood T cells of OLP patients was significantly higher than that in controls, especially in female and middle-aged (30–50 years old) OLP patients. In addition,ATG9BmRNA levels were significantly lower in nonerosive OLP patients. However, no significant differences were found in the expression ofHGS,ESR1, andSNCAbetween OLP patients and controls. Taken together, dysregulation of T cell autophagy may be involved in immune response of OLP and may be correlated with clinical patterns.

Download Full-text