Development of competitive inhibition ELISA as an effective potency test to analyze human rabies vaccines and assessment of the antigenic epitope of rabies glycoprotein

2020 ◽  
pp. 112939
Author(s):  
Dipen Soni ◽  
Itishree Sahoo ◽  
Asha D. Mallya ◽  
Praveen Kamthe ◽  
Ashish Sahai ◽  
...  
Keyword(s):  
Competitive Inhibition ◽  
Human Rabies ◽  
Antigenic Epitope ◽  
Inhibition Elisa ◽  
Rabies Glycoprotein

Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goats

2020 ◽  
Vol 284 ◽  
pp. 109201
Author(s):  
María Belén Novoa ◽  
Nerina Patricia Aguirre ◽  
Nadia Ormaechea ◽  
Sebastián Palmero ◽  
Lisandro Rouzic ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Competitive Inhibition ◽  
Neospora Caninum ◽  
Field Evaluation ◽  
Sheep And Goats ◽  
Inhibition Elisa

Detection of Theileria equi and Babesia caballi infections in Venezuelan horses using Competitive-Inhibition ELISA and PCR

2013 ◽  
Vol 196 (1-2) ◽  
pp. 37-43 ◽  
Author(s):  
Romel Rosales ◽  
Ariadna Rangel-Rivas ◽  
América Escalona ◽  
Luis Segundo Jordan ◽  
Mary Isabel Gonzatti ◽  
...  
Keyword(s):  
Competitive Inhibition ◽  
Theileria Equi ◽  
Babesia Caballi ◽  
Inhibition Elisa

scholarly journals Evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti–Neospora caninum antibodies in cattle

2020 ◽  
Vol 32 (3) ◽  
pp. 401-408
Author(s):  
María B. Novoa ◽  
Beatriz S. Valentini ◽  
Macarena Sarli ◽  
Susana M. Torioni-de-Echaide ◽  
María E. Primo ◽  
...  
Keyword(s):  
Large Scale ◽  
Competitive Inhibition ◽  
Neospora Caninum ◽  
Management Strategies ◽  
Protozoan Parasite ◽  
Economic Losses ◽  
Serum Samples ◽  
Beef Cow ◽  
Inhibition Elisa ◽  
Scale Detection

Neospora caninum is a protozoan parasite that causes abortion and important economic losses in cattle worldwide. There are no treatments or vaccines available; disease control is based on diagnosis and herd management strategies. We developed, validated, and evaluated under field conditions a competitive inhibition ELISA based on the truncated SAG1 protein (tSAG1), expressed in Escherichia coli, and the RafNeo5 monoclonal antibody (ciELISAtSAG1). A criterion based on the 3-y sequential serologic analysis of 230 dairy cows by IFAT was used as the gold standard. The assay was validated using 860 serum samples from cows that were consistently positive or negative by IFAT throughout the study period. ciELISAtSAG1 was then used to evaluate the prevalence of neosporosis in 16 beef cow herds (22 samples per herd, 352 total samples). The results were compared with those from IFAT and a commercial cELISA (cELISAVMRD). The ciELISAtSAG1 cutoff was ≥ 29%I, with a diagnostic sensitivity of 98.7% (95% CI = 96.8–99.7%) and a diagnostic specificity of 97.9% (95% CI = 96.4–99.0%). Concordance among IFAT, cELISAVMRD, and ciELISAtSAG1 was 90.3%. The agreement (κ) between ciELISAtSAG1 and the other 2 tests was ≥ 0.81. The overall prevalence of neosporosis in the 16 beef herds was 30% (range: 5–60%). The ciELISAtSAG1 could be useful for large-scale detection of anti– N. caninum antibodies in cattle and seroepidemiologic investigations, given its appropriate sensitivity and specificity, and the simplicity of production.

PREVALENCE OF ANTIBODY TO MALIGNANT CATARRHAL FEVER VIRUS IN WILD AND DOMESTIC RUMINANTS BY COMPETITIVE-INHIBITION ELISA

1996 ◽  
Vol 32 (3) ◽  
pp. 437-443 ◽  
Author(s):  
Hong Li ◽  
David T. Shen ◽  
David A. Jessup ◽  
Donald P. Knowles ◽  
John R. Gorham ◽  
...  
Keyword(s):  
Competitive Inhibition ◽  
Fever Virus ◽  
Malignant Catarrhal Fever ◽  
Inhibition Elisa ◽  
Domestic Ruminants

scholarly journals Immunological discrimination of diverse forms of human alpha 1-proteinase inhibitor.

1996 ◽  
Vol 43 (3) ◽  
pp. 481-487 ◽  
Author(s):  
I Wawrzos ◽  
Y Kitagawa ◽  
H Kołoczek
Keyword(s):  
Proteinase Inhibitor ◽  
Competitive Inhibition ◽  
Immunological Response ◽  
Cross Reactivity ◽  
Reactive Site ◽  
Latent Form ◽  
Mild Conditions ◽  
Inhibition Elisa ◽  
Inhibition Data ◽  
Elisa Data

The immunodiffusion cross-reactivity and competitive inhibition ELISA assays were used for immunological differentiation of latent form, cleaved form and guanidinium hydrochloride (GuHCl) induced polymer of human alpha 1-proteinase inhibitor (alpha 1-PI). Under the conditions studied, the differences between latent form and GuHCl-induced polymers of the inhibitor in terms of immunological response were estimated to amount to about 30% and differences between latent and cleaved alpha 1-PI to about 50%. The immunodiffusion and ELISA data for citrate-induced polymers suggest that in their structure the latent molecule is involved. On the basis of competitive inhibition data, we suggest that the alpha 1-PI protein polymerisation involves insertion of the reactive-site loop (RSL) into the A-sheet under mild conditions and that in the latent form of the inhibitor RSL is incompletely inserted into the A-sheet.

scholarly journals Prevalence of Neospora spp. in donkeys in China

Parasite ◽  
2018 ◽  
Vol 25 ◽  
pp. 16 ◽  
Author(s):  
Wei Cong ◽  
Lan-Bi Nie ◽  
Si-Yuan Qin ◽  
Wei-Li Wang ◽  
Ai-Dong Qian ◽  
...  
Keyword(s):  
Risk Factors ◽  
Statistical Analysis ◽  
Competitive Inhibition ◽  
Significant Risk ◽  
Elisa Kit ◽  
Inhibition Elisa

This study was conducted to detect specific anti-Neospora antibodies using a commercial competitive-inhibition ELISA kit, and to evaluate the risk factors for Neospora spp. infection. Out of a total of 2,228 donkey sera collected in three provinces in China, 211 (9.5%) were found to be positive for anti-Neospora antibodies. Statistical analysis revealed that age (p = 0.019, OR = 1.62, 95%CI: 1.08-2.44), feeding status (p < 0.001, OR = 3.79, 95%CI: 2.65-5.43), miscarriage history (p = 0.006, OR = 2.56, 95%CI: 1.27-4.01), and contact with dogs (p < 0.001, OR = 2.69, 95%CI: 1.86-3.88) were significant risk factors for Neospora spp. infection. This is the first evidence of Neospora infection in donkeys in China.

Two Expression Vectors, Designated as pGEX-CDON and pC89S4-CDON, for Producing GST-CDON and pVIII-CDON Fusion Proteins

2013 ◽  
Vol 726-731 ◽  
pp. 505-510
Author(s):  
Fu Yong Xu ◽  
Ren Rong Liu ◽  
Ling Xu ◽  
Xue Mei Qiu ◽  
Li Xin Zhu
Keyword(s):  
Linear Equation ◽  
Fusion Proteins ◽  
Competitive Inhibition ◽  
Elisa Test ◽  
Linear Range ◽  
Expression Vectors ◽  
Random Peptide Library ◽  
Random Peptide ◽  
Inhibition Elisa ◽  
Better Than

Deoxynivalenol (DON) mimotope, designated as CDON, is an epitope (CMRPWLQ) immunoscreened from a phage-displayed random peptide library. In order to replace the conjugated toxin with non-toxic recombinant proteins in ELISA, two novel expression vectors, which were designated as plasmid pGEX-CDON and phagemid pC89S4-CDON for producing GST-CDON and pVIII-CDON fusion proteins in E.coli were constructed. After purification, both GST-CDON and pVIII-CDON fusion proteins show good reactogenicity with an anti-DON antibody in a competitive inhibition ELISA test. When GST-CDON was used as coating antigen, the linear range of the competitive inhibition ELISA is from 62ng/ml to 410ng/ml, the linear equation is Y= 186.6-23.87Ln (X), IC50 is 194ng/ml. For pVIII-CDON as coating protein, the linear range of the competitive inhibition ELISA is from 20ng/ml to 470ng/ml, the linear equation is Y = 161.3-25.49Ln (X), R2=0.9962, IC50 is 94ng/ml. ELISA analysis and comparison show the reactogenicity and specificity of pVIII-CDON binding to anti-DON antibody are better than GST-CDON fusion protein. The pVIII-CDON is promising in establishing an ELISA without the use of the toxic mycotoxin conjugate.

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