Saponins do not affect the ecdysteroid receptor complex but cause membrane permeation in insect culture cell lines

2012 ◽  
Vol 58 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Ellen De Geyter ◽  
Luc Swevers ◽  
Thomas Soin ◽  
Danny Geelen ◽  
Guy Smagghe
Keyword(s):  
Cell Lines ◽  
Culture Cell ◽  
Receptor Complex ◽  
Membrane Permeation ◽  
Ecdysteroid Receptor ◽  
Insect Culture

Juvenile hormone analogs do not affect directly the activity of the ecdysteroid receptor complex in insect culture cell lines

2008 ◽  
Vol 54 (2) ◽  
pp. 429-438 ◽  
Author(s):  
Thomas Soin ◽  
Luc Swevers ◽  
Hadi Mosallanejad ◽  
Rodica Efrose ◽  
Vassiliki Labropoulou ◽  
...  
Keyword(s):  
Juvenile Hormone ◽  
Cell Lines ◽  
Culture Cell ◽  
Receptor Complex ◽  
Ecdysteroid Receptor ◽  
Insect Culture ◽  
Hormone Analogs

Cold storage of biopsies from wild endangered native Chilean species in field conditions and subsequent isolation of primary culture cell lines

2008 ◽  
Vol 44 (8-9) ◽  
pp. 309-320 ◽  
Author(s):  
Heribelt Tovar ◽  
Felipe Navarrete ◽  
Lleretny Rodríguez ◽  
Oscar Skewes ◽  
Fidel Ovidio Castro
Keyword(s):  
Primary Culture ◽  
Cell Lines ◽  
Cold Storage ◽  
Culture Cell ◽  
Field Conditions ◽  
Primary Culture Cell

scholarly journals Use of Tissue Culture Cell Lines to Evaluate HIV Antiviral Resistance

2008 ◽  
Vol 24 (7) ◽  
pp. 957-967 ◽  
Author(s):  
Halina Krowicka ◽  
James E. Robinson ◽  
Rebecca Clark ◽  
Shannon Hager ◽  
Stephanie Broyles ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Cell Lines ◽  
Culture Cell ◽  
Antiviral Resistance ◽  
Tissue Culture Cell

Attachment of Salmonella to mammalian cells in vitro

1983 ◽  
Vol 29 (12) ◽  
pp. 1731-1735 ◽  
Author(s):  
Clifford S. Mintz ◽  
Dean O. Cliver ◽  
R. H. Deibel
Keyword(s):  
Cell Lines ◽  
Mammalian Cells ◽  
Culture Cell ◽  
Intestinal Cell ◽  
Tissue Culture Cell ◽  
Bacterial Cells ◽  
Intestinal Cell Line ◽  
Hela Cell Lines ◽  
High Concentrations

The attachment of Salmonella typhimurium strain PHL67342 to several mammalian tissue culture cell lines was investigated. Strain PHL67342 failed to attach in significant numbers to the Buffalo green monkey (BGM), swine testicular (ST), and HeLa cell lines. Significant attachment was observed with the Henle intestinal cell line. Log-phase cells of strain PHL67342 attached in greatest numbers to the Henle cells after 45 min of incubation at 37 °C. Attachment to the Henle cells was not affected by D-mannose or D-galactose, but was markedly inhibited by high concentrations of alpha-methyl-D-mannoside. Also, Salmonella lipopolysaccharide had no effect on the attachment of strain PHL67342 to the Henle cells. Fimbriae were not detected on the bacterial cells used in the adherence experiments. These results suggest that some bacterial factor(s) other than fimbriae and lipopolysaccharide mediate the attachment of strain PHL67342 to the Henle cells.

scholarly journals The T cell antigen receptor complex expressed on normal peripheral blood CD4-, CD8- T lymphocytes. A CD3-associated disulfide-linked gamma chain heterodimer.

1987 ◽  
Vol 165 (4) ◽  
pp. 1076-1094 ◽  
Author(s):  
L L Lanier ◽  
N A Federspiel ◽  
J J Ruitenberg ◽  
J H Phillips ◽  
J P Allison ◽  
...  
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
Cell Lines ◽  
Peripheral Blood ◽  
Antigen Receptor ◽  
Receptor Complex ◽  
T Cell Antigen Receptor ◽  
Normal Peripheral Blood ◽  
Cell Antigen Receptor ◽  
Cell Antigen

IL-2-dependent cell lines were established from normal peripheral blood T lymphocytes that express neither CD4 nor CD8 differentiation antigens. CD3+,4-,8- cell lines from 15 different donors failed to react with WT31, an mAb directed against the T cell antigen receptor alpha/beta heterodimer. Anti-Leu-4 mAb was used to isolate the CD3/T cell antigen receptor complex from 125I-labeled CD3+,4-,8- (WT31-) T cells. Using detergent conditions that preserved the CD3/T cell antigen receptor complex, an approximately 90 kD disulfide-linked heterodimer, composed of approximately 45- and approximately 40- (or approximately 37-) kD subunits, was coimmunoprecipitated with the invariant 20-29-kD CD3 complex. Analysis of these components by nonequilibrium pH gradient electrophoresis indicated that the approximately 40-kD and approximately 37-kD subunits were similar, and quite distinct from the more basic approximately 45-kD subunit. None of these three subunits reacted with an antibody directed against a beta chain framework epitope. Heteroantiserum against a T cell receptor gamma chain peptide specifically reacted with both the approximately 37- and approximately 40-kD CD3-associated proteins, but not with the approximately 45-kD subunit. CD3+,4-,8- cells failed to transcribe substantial amounts of functional 1.3-kb beta or 1.6-kb alpha mRNA, but produced abundant 1.6-kb gamma mRNA. Southern blot analysis revealed that these CD3+,4-,8- cell lines rearranged both gamma and beta genes, and indicated that the populations were polyclonal. The expression of a CD3-associated disulfide-linked heterodimer on CD3+,4-,8- T cell lines established from normal, adult peripheral blood contrasts with prior reports describing a CD3-associated non-disulfide-linked heterodimer on CD3+/WT31- cell lines established from thymus and peripheral blood obtained from patients with immunodeficiency diseases. We propose that this discrepancy may be explained by preferential usage of the two C gamma genes in T lymphocytes.

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