Development of a nucleic acid lateral flow strip for rapid, visual detection of Nosema bombycis in silkworm eggs

2019 ◽  
Vol 164 ◽  
pp. 59-65 ◽  
Author(s):  
Zhangshuai He ◽  
Qi Ni ◽  
Yue Song ◽  
Rui Wang ◽  
Yunlin Tang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Visual Detection ◽  
Lateral Flow ◽  
Lateral Flow Strip ◽  
Nosema Bombycis

scholarly journals Visual detection of gene mutations based on isothermal strand-displacement polymerase reaction and lateral flow strip

2012 ◽  
Vol 31 (1) ◽  
pp. 310-315 ◽  
Author(s):  
Yuqing He ◽  
Kang Zeng ◽  
Sanquan Zhang ◽  
Anant S. Gurung ◽  
Meenu Baloda ◽  
...  
Keyword(s):  
Visual Detection ◽  
Gene Mutations ◽  
Lateral Flow ◽  
Strand Displacement ◽  
Lateral Flow Strip

An enzyme-amplified lateral flow strip biosensor for visual detection of MicroRNA-224

Talanta ◽  
2016 ◽  
Vol 146 ◽  
pp. 648-654 ◽  
Author(s):  
Xuefei Gao ◽  
Li-Ping Xu ◽  
Tingting Wu ◽  
Yongqiang Wen ◽  
Xinlei Ma ◽  
...  
Keyword(s):  
Visual Detection ◽  
Lateral Flow ◽  
Lateral Flow Strip

Disposable Nucleic Acid Biosensors Based on Gold Nanoparticle Probes and Lateral Flow Strip

2009 ◽  
Vol 81 (4) ◽  
pp. 1660-1668 ◽  
Author(s):  
Xun Mao ◽  
Yunqing Ma ◽  
Aiguo Zhang ◽  
Lurong Zhang ◽  
Lingwen Zeng ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Gold Nanoparticle ◽  
Lateral Flow ◽  
Lateral Flow Strip ◽  
Nanoparticle Probes

scholarly journals Gold Nanoparticle Coated Silica Nanorods for Sensitive Visual Detection of microRNA on a Lateral Flow Strip Biosensor

2016 ◽  
Vol 32 (6) ◽  
pp. 617-622 ◽  
Author(s):  
Sunitha TAKALKAR ◽  
Hui XU ◽  
Jiao CHEN ◽  
Kwaku BARYEH ◽  
Wanwei QIU ◽  
...  
Keyword(s):  
Gold Nanoparticle ◽  
Visual Detection ◽  
Lateral Flow ◽  
Lateral Flow Strip ◽  
Silica Nanorods

Lateral flow strip for visual detection of K-ras mutations based on allele-specific PCR

2016 ◽  
Vol 38 (10) ◽  
pp. 1709-1714 ◽  
Author(s):  
Cong Wang ◽  
Xiaomin Chen ◽  
Yuying Wu ◽  
Hao Li ◽  
Yu Wang ◽  
...  
Keyword(s):  
Visual Detection ◽  
Lateral Flow ◽  
Lateral Flow Strip ◽  
Ras Mutations ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

scholarly journals Visual Detection of Dengue-1 RNA Using Gold Nanoparticle-Based Lateral Flow Biosensor

Diagnostics ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 74 ◽  
Author(s):  
Flora M. Yrad ◽  
Josephine M. Castañares ◽  
Evangelyn C. Alocilja
Keyword(s):  
Nucleic Acid ◽  
Gold Nanoparticle ◽  
Disease Surveillance ◽  
Visual Detection ◽  
Dengue Infection ◽  
Viral Disease ◽  
Lateral Flow ◽  
Resource Limited ◽  
Sandwich Type ◽  
Human Sera

Dengue is a rapidly spreading mosquito-borne viral disease. Early diagnosis is important for clinical screening, medical management, and disease surveillance. The objective of this study was to develop a colorimetric lateral flow biosensor (LFB) for the visual detection of dengue-1 RNA using dextrin-capped gold nanoparticle (AuNP) as label. The detection was based on nucleic acid sandwich-type hybridization among AuNP-labeled DNA reporter probe, dengue-1 target RNA, and dengue-1 specific DNA capture probe immobilized on the nitrocellulose membrane. Positive test generated a red test line on the LFB strip, which enabled visual detection. The optimized biosensor has a cut-off value of 0.01 µM using synthetic dengue-1 target. Proof-of-concept application of the biosensor detected dengue-1 virus in pooled human sera with a cut-off value of 1.2 × 104 pfu/mL. The extracted viral RNA, when coupled with nucleic acid sequence-based amplification (NASBA), was detected on the LFB in 20 min. This study first demonstrates the applicability of dextrin-capped AuNP as label for lateral flow assay. The biosensor being developed provides a promising diagnostic platform for early detection of dengue infection in high-risk resource-limited areas.

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