Disposable Nucleic Acid Biosensors Based on Gold Nanoparticle Probes and Lateral Flow Strip

2009 ◽  
Vol 81 (4) ◽  
pp. 1660-1668 ◽  
Author(s):  
Xun Mao ◽  
Yunqing Ma ◽  
Aiguo Zhang ◽  
Lurong Zhang ◽  
Lingwen Zeng ◽  
...  
2011 ◽  
Vol 26 (5) ◽  
pp. 2018-2024 ◽  
Author(s):  
Yuqing He ◽  
Sanquan Zhang ◽  
Xibao Zhang ◽  
Meenu Baloda ◽  
Anant S. Gurung ◽  
...  

2019 ◽  
Vol 164 ◽  
pp. 59-65 ◽  
Author(s):  
Zhangshuai He ◽  
Qi Ni ◽  
Yue Song ◽  
Rui Wang ◽  
Yunlin Tang ◽  
...  

2012 ◽  
Vol 97 ◽  
pp. 294-296 ◽  
Author(s):  
Cheng-Han Chao ◽  
Chung-Shu Wu ◽  
Chung-Chih Huang ◽  
Jie-Chian Liang ◽  
Hsiao-Ting Wang ◽  
...  

2007 ◽  
Vol 90 (2) ◽  
pp. 023903 ◽  
Author(s):  
Rodrigo Martins ◽  
Pedro Baptista ◽  
Leandro Raniero ◽  
Gonçalo Doria ◽  
Leonardo Silva ◽  
...  

2020 ◽  
Vol 12 (25) ◽  
pp. 3210-3217
Author(s):  
Jie Liu ◽  
Shanshan Song ◽  
Aihong Wu ◽  
Xiaoling Wu ◽  
Jing Xiao ◽  
...  

Dinitolmide is a nitro amide coccidiostat used in poultry feed, and is a potential threat to the environment and human health.


2016 ◽  
Vol 32 (6) ◽  
pp. 617-622 ◽  
Author(s):  
Sunitha TAKALKAR ◽  
Hui XU ◽  
Jiao CHEN ◽  
Kwaku BARYEH ◽  
Wanwei QIU ◽  
...  

2018 ◽  
Vol 2 (10) ◽  
pp. 1900-1910 ◽  
Author(s):  
Zhongxing Wang ◽  
Li Sun ◽  
Liqiang Liu ◽  
Hua Kuang ◽  
Liguang Xu ◽  
...  

We developed an ultrasensitive gold nanoparticle-based multicomponent lateral-flow strip assay for the simultaneous detection of seventeen hormone drugs from three classes: nandrolone (NR) and its analogues, dexamethasone (DEX) and its analogues, and hexestrol (HES) and its analogues.


Diagnostics ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 74 ◽  
Author(s):  
Flora M. Yrad ◽  
Josephine M. Castañares ◽  
Evangelyn C. Alocilja

Dengue is a rapidly spreading mosquito-borne viral disease. Early diagnosis is important for clinical screening, medical management, and disease surveillance. The objective of this study was to develop a colorimetric lateral flow biosensor (LFB) for the visual detection of dengue-1 RNA using dextrin-capped gold nanoparticle (AuNP) as label. The detection was based on nucleic acid sandwich-type hybridization among AuNP-labeled DNA reporter probe, dengue-1 target RNA, and dengue-1 specific DNA capture probe immobilized on the nitrocellulose membrane. Positive test generated a red test line on the LFB strip, which enabled visual detection. The optimized biosensor has a cut-off value of 0.01 µM using synthetic dengue-1 target. Proof-of-concept application of the biosensor detected dengue-1 virus in pooled human sera with a cut-off value of 1.2 × 104 pfu/mL. The extracted viral RNA, when coupled with nucleic acid sequence-based amplification (NASBA), was detected on the LFB in 20 min. This study first demonstrates the applicability of dextrin-capped AuNP as label for lateral flow assay. The biosensor being developed provides a promising diagnostic platform for early detection of dengue infection in high-risk resource-limited areas.


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