scholarly journals Cooperativeness of the Higher Chromatin Structure of the β-Globin Locus Revealed by the Deletion Mutations of DNase I Hypersensitive site 3 of the LCR

2007 ◽  
Vol 365 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Xiangdong Fang ◽  
Ping Xiang ◽  
Wenxuan Yin ◽  
George Stamatoyannopoulos ◽  
Qiliang Li
Keyword(s):  
Chromatin Structure ◽  
Dnase I ◽  
Hypersensitive Site ◽  
Deletion Mutations ◽  
Dnase I Hypersensitive Site

scholarly journals AnCF, the CCAAT Binding Complex ofAspergillus nidulans, Is Essential for the Formation of a DNase I-Hypersensitive Site in the 5′ Region of theamdS Gene

1999 ◽  
Vol 19 (10) ◽  
pp. 6523-6531 ◽  
Author(s):  
Frank M. Narendja ◽  
Meryl A. Davis ◽  
Michael J. Hynes
Keyword(s):  
Chromatin Structure ◽  
Gene Deletion ◽  
Critical Role ◽  
Dnase I ◽  
Open Chromatin ◽  
Hypersensitive Site ◽  
Transcriptional Start Point ◽  
Dnase I Hypersensitive Site ◽  
Ccaat Box ◽  
Ccaat Sequence

ABSTRACT The CCAAT sequence in the amdS promoter ofAspergillus nidulans is recognized by AnCF, a complex consisting of the three evolutionary conserved subunits HapB, HapC, and HapE. In this study we have investigated the effect of AnCF on the chromatin structure of the amdS gene. The AnCF complex and the CCAAT sequence were found to be necessary for the formation of a nucleosome-free, DNase I-hypersensitive region in the 5′ region of theamdS gene. Deletion of the hapE gene results in loss of the DNase I-hypersensitive site, and the positioning of nucleosomes over the transcriptional start point is lost. Likewise, a point mutation in the CCAAT motif, as well as a 530-bp deletion which removes the CCAAT box, results in the loss of the DNase I-hypersensitive region. The DNase I-hypersensitive region and the nucleosome positioning can be restored by insertion of a 35-bp oligonucleotide carrying the CCAAT motif. A DNase I-hypersensitive region has been found in the CCAAT-containing fmdS gene and was also hapE dependent. These data indicate a critical role for the AnCF complex in establishing an open chromatin structure in A. nidulans.

scholarly journals Alternative inducers of the rat metallothionein I gene cause distinct changes in chromatin structure in the 5' region of the gene.

1986 ◽  
Vol 6 (7) ◽  
pp. 2576-2581 ◽  
Author(s):  
S J Taplitz ◽  
K L Calame ◽  
H R Herschman
Keyword(s):  
Chromatin Structure ◽  
Hepatoma Cell ◽  
Regulatory Elements ◽  
Dnase I ◽  
Hepatoma Cell Line ◽  
Hypersensitive Site ◽  
Level Control ◽  
Dnase I Hypersensitive Site ◽  
Rat Hepatoma Cell Line ◽  
I Gene

We examined the chromatin structure of the rat metallothionein I gene, both in uninduced cells and in cells induced by heavy metals or dexamethasone, using hypersensitivity to DNase I as an assay. The metallothionein I gene of the H4IIE rat hepatoma cell line, expressed at basal level, has a single DNase I-hypersensitive site. This site maps between putative hormone and basal level control sequences. Induction of the gene by cadmium or zinc resulted in the appearance of a new hypersensitive site near the start site of transcription, in a region near the metal-regulatory elements. In contrast, induction of the metallothionein I gene by dexamethasone did not alter the basal pattern of hypersensitivity. Thus, different mechanisms of induction of metallothionein transcription lead to distinct alterations in the chromatin containing the 5' sequences regulating the expression of this gene.

Alternative inducers of the rat metallothionein I gene cause distinct changes in chromatin structure in the 5' region of the gene

1986 ◽  
Vol 6 (7) ◽  
pp. 2576-2581
Author(s):  
S J Taplitz ◽  
K L Calame ◽  
H R Herschman
Keyword(s):  
Chromatin Structure ◽  
Hepatoma Cell ◽  
Regulatory Elements ◽  
Dnase I ◽  
Hepatoma Cell Line ◽  
Hypersensitive Site ◽  
Level Control ◽  
Dnase I Hypersensitive Site ◽  
Rat Hepatoma Cell Line ◽  
I Gene

We examined the chromatin structure of the rat metallothionein I gene, both in uninduced cells and in cells induced by heavy metals or dexamethasone, using hypersensitivity to DNase I as an assay. The metallothionein I gene of the H4IIE rat hepatoma cell line, expressed at basal level, has a single DNase I-hypersensitive site. This site maps between putative hormone and basal level control sequences. Induction of the gene by cadmium or zinc resulted in the appearance of a new hypersensitive site near the start site of transcription, in a region near the metal-regulatory elements. In contrast, induction of the metallothionein I gene by dexamethasone did not alter the basal pattern of hypersensitivity. Thus, different mechanisms of induction of metallothionein transcription lead to distinct alterations in the chromatin containing the 5' sequences regulating the expression of this gene.

scholarly journals Mi2β Shows Chromatin Enzyme Specificity by Erasing a DNase I-hypersensitive Site Established by ACF

2009 ◽  
Vol 284 (12) ◽  
pp. 7533-7541 ◽  
Author(s):  
Haruhiko Ishii ◽  
Hansen Du ◽  
Zhaoqing Zhang ◽  
Angus Henderson ◽  
Ranjan Sen ◽  
...  
Keyword(s):  
Dnase I ◽  
Enzyme Specificity ◽  
Hypersensitive Site ◽  
Dnase I Hypersensitive Site

scholarly journals Interleukin 4 induces changes in the chromatin structure of the gamma 1 switch region in resting B cells before switch recombination.

1990 ◽  
Vol 172 (1) ◽  
pp. 375-378 ◽  
Author(s):  
M T Berton ◽  
E S Vitetta
Keyword(s):  
B Cells ◽  
Chromatin Structure ◽  
Transcriptional Activity ◽  
Interleukin 4 ◽  
Open Chromatin ◽  
T Helper ◽  
Hypersensitive Site ◽  
Switch Region ◽  
Dnase I Hypersensitive Site ◽  
Selection Of

Interleukin 4 (IL-4) can induce the expression of IgG1 in sIgG- murine B cells stimulated with mitogens or through a cognate interaction with T helper (Th) cells. We have investigated the molecular basis for the IL-4-induced switch to IgG1 in lipopolysaccharide (LPS)-stimulated murine B cells and have previously shown that IL-4 induces transcription of the gamma 1 switch region before switch recombination. We now demonstrate that IL-4 induces a DNase I hypersensitive site at the 5' end of the gamma 1 switch region in resting B cells. LPS is not required, but it enhances induction. Hence, the interaction of IL-4 with its receptor results in increased accessibility of the gamma 1 switch region. The more open chromatin structure and increased transcriptional activity may be important in the selection of this region for switch recombination.

A Binding Protein to the DNase I Hypersensitive Site II in HLA-DRα Gene Was Identified as NF90

Biochemistry ◽  
1999 ◽  
Vol 38 (11) ◽  
pp. 3355-3361 ◽  
Author(s):  
Shuji Sakamoto ◽  
Keiko Morisawa ◽  
Katsuya Ota ◽  
Jing Nie ◽  
Taketoshi Taniguchi
Keyword(s):  
Binding Protein ◽  
Dnase I ◽  
Hypersensitive Site ◽  
Dnase I Hypersensitive Site
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