Critical features of periodontal flaps with regard to blood clot stability: A review

2021 ◽  
Author(s):  
Muhammad Saad Shaikh ◽  
Muhammad Sohail Zafar ◽  
Flavio Pisani ◽  
Mohid Abrar Lone ◽  
Yasser Riaz Malik
Keyword(s):  
Blood Clot ◽  
Clot Stability

scholarly journals Blood clot stability and bone formation following maxillary sinus membrane elevation and space maintenance by means of immediate implant placement in humans. A computed tomography study

2019 ◽  
Vol 47 (11) ◽  
pp. 1803-1808 ◽  
Author(s):  
Elton Gonçalves Zenóbio ◽  
Liziany David Cardoso ◽  
Leandro Junqueira de Oliveira ◽  
Mário Nazareno Favato ◽  
Flávio Ricardo Manzi ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Bone Formation ◽  
Maxillary Sinus ◽  
Blood Clot ◽  
Implant Placement ◽  
Space Maintenance ◽  
Immediate Implant ◽  
Sinus Membrane Elevation ◽  
Sinus Membrane ◽  
Clot Stability

scholarly journals Fibrinogen αC-subregions critically contribute blood clot fibre growth, mechanical stability and resistance to fibrinolysis

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Helen McPherson ◽  
Cedric Duval ◽  
Stephen R Baker ◽  
Matthew S Hindle ◽  
Lih T Cheah ◽  
...  
Keyword(s):  
Mechanical Strength ◽  
Blood Coagulation ◽  
Mechanical Stability ◽  
Blood Clot ◽  
C Terminus ◽  
Fibre Growth ◽  
Clot Structure ◽  
Clot Stability ◽  
Longitudinal Fibre

Fibrinogen is essential for blood coagulation. The C-terminus of the fibrinogen α-chain (αC-region) is composed of an αC-domain and αC-connector. Two recombinant fibrinogen variants (α390 and α220) were produced to investigate the role of subregions in modulating clot stability and resistance to lysis. The α390 variant, truncated before the αC-domain, produced clots with a denser structure and thinner fibres. In contrast, the α220 variant, truncated at the start of the αC-connector, produced clots that were porous with short, stunted fibres and visible fibre ends. These clots were mechanically weak and susceptible to lysis. Our data demonstrate differential effects for the αC-subregions in fibrin polymerisation, clot mechanical strength, and fibrinolytic susceptibility. Furthermore, we demonstrate that the αC-subregions are key for promoting longitudinal fibre growth. Together, these findings highlight critical functions of the αC-subregions in relation to clot structure and stability, with future implications for development of novel therapeutics for thrombosis.

scholarly journals Fibrinogen αC-subregions critically contribute blood clot fibre growth, mechanical stability and resistance to fibrinolysis

2021 ◽  
Author(s):  
Helen McPherson ◽  
Cedric Duval ◽  
Stephen R Baker ◽  
Matthew S Hindle ◽  
Lih T Cheah ◽  
...  
Keyword(s):  
Mechanical Strength ◽  
Blood Coagulation ◽  
Mechanical Stability ◽  
Blood Clot ◽  
C Terminus ◽  
Fibre Growth ◽  
Clot Structure ◽  
Clot Stability ◽  
Longitudinal Fibre

Fibrinogen is essential for blood coagulation. The C-terminus of the fibrinogen α-chain (αC-region) is composed of an αC-domain and αC-connector. Two recombinant fibrinogen variants (α390 and α220) were produced to investigate the role of subregions in modulating clot stability and resistance to lysis. The α390 variant, truncated before the αC-domain, produced clots with a denser structure and thinner fibres. In contrast, the α220 variant, truncated at the start of the αC-connector, produced clots that were porous with short stunted fibres and visible fibre ends. These clots were mechanically weak and susceptible to lysis. Our data demonstrate differential effects for the αC-subregions in fibrin polymerisation, clot mechanical strength, and fibrinolytic susceptibility. Furthermore, we demonstrate that the αC-subregions are key for promoting longitudinal fibre growth. Together, these findings highlight critical functions of the αC-subregions in relation to clot structure and stability, with future implications for development of novel therapeutics for thrombosis.

The Coagulation System and Blood Clot Stability

2021 ◽  
pp. 29-35
Author(s):  
Nikita Nayyar ◽  
Haig Mannasian ◽  
Longqiu Yang ◽  
Henry Liu
Keyword(s):  
Blood Clot ◽  
Coagulation System ◽  
Clot Stability

FDA: OCs With Drospirenone May Raise Blood Clot Risk

2012 ◽  
Vol 42 (8) ◽  
pp. 29
Author(s):  
ELIZABETH MECHCATIE
Keyword(s):  
Blood Clot

A Monoclonal Antibody-Based Enzyme Immunoassay for Fibrin Degradation Products in Plasma

1988 ◽  
Vol 59 (02) ◽  
pp. 310-315 ◽  
Author(s):  
P W Koppert ◽  
E Hoegee-de Nobel ◽  
W Nieuwenhuizen
Keyword(s):  
Enzyme Immunoassay ◽  
Degradation Products ◽  
Blood Clot ◽  
Tissue Type ◽  
Fibrin Degradation Products ◽  
Type Plasminogen Activator ◽  
Strong Positive Reaction ◽  
Sandwich Type ◽  
Capture Antibody

SummaryWe have developed a sandwich-type enzyme immunoassay (EIA) for the quantitation of fibrin degradation products (FbDP) in plasma with a time-to-result of only 45 minutes.* The assay is based on the combination of the specificities of two monoclonal antibodies (FDP-14 and DD-13), developed in our institute. FDP-14, the capture antibody, binds both fibrinogen degradation products (FbgDP) and FbDP, but does not react with the parent fibrin(ogen) molecules. It has its epitope in the E-domain of the fibrinogen molecule on the Bβ-chain between amino acids 54-118. Antibody DD-13 was raised using D-dimer as antigen and is used as a tagging antibody, conjugated with horse-radish peroxidase. A strong positive reaction is obtained with a whole blood clot lysate (lysis induced by tissue-type plasminogen activator) which is used as a standard. The EIA does virtually not detect FbgDP i. e. purified fragments X, Y, or FbgDP generated in vitro in plasma by streptokinase treatment. This indicates that the assay is specific for fibrin degradation products.We have successfully applied this assay to the plasma of patients with a variety of diseased states. In combination with the assay previously developed by us for FbgDP and for the total amount of FbgDP + FbDP (TDP) in plasma, we are now able to study the composition of TDP in patients plasma in terms of FbgDP and FbDP.

Dependence of Blood Clot Lysis on the Mode of Transport of Urokinase into the Clot - A Magnetic Resonance Imaging Study in Vitro

1991 ◽  
Vol 65 (05) ◽  
pp. 549-552 ◽  
Author(s):  
A Blinc ◽  
G Planinšič ◽  
D Keber ◽  
O Jarh ◽  
G Lahajnar ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Pressure Difference ◽  
Volume Flow Rate ◽  
Blood Clot ◽  
Linear Regression Analysis ◽  
Imaging Study ◽  
Clot Lysis ◽  
Resonance Imaging

SummaryMagnetic resonance imaging was employed to study the dependence of clot lysing patterns on two different modes of transport of urokinase into whole blood clots. In one group of clots (nonperfused clots, n1 = 10), access of urokinase to the fibrin network was possible by diffusion only, whereas in the other group (perfused clots, n2 = 10) bulk flow of plasma containing urokinase was instituted through occlusive clots by a pressure difference of 3 .7 kPa (37 cm H2O) across 3 cm long clots with a diameter of 4 mm. It was determined separately that this pressure difference resulted in a volume flow rate of 5.05 ± 2.4 × 10−2 ml/min through occlusive clots. Perfused clots diminished in size significantly in comparison to nonperfused ones already after 20 min (p <0.005). Linear regression analysis of two-dimensional clot sizes measured by MRI showed that the rate of lysis was more than 50-times faster in the perfused group in comparison to the nonperfused group. It was concluded that penetration of the thrombolytic agent into clots by perfusion is much more effective than by diffusion. Our results might have some implications for understanding the differences in lysis of arterial and venous thrombi.

Urokinase Evaluated with the Experimental Radioactive Embolus

1967 ◽  
Vol 17 (03/04) ◽  
pp. 405-411
Author(s):  
M Hume
Keyword(s):  
Animal Experiment ◽  
Blood Clot ◽  
In Vitro Testing ◽  
Effective Agent

SummaryUrokinase and urokinase-activated plasmin have been given to the dog and rabbit. A thrombolytic state has been induced. Purified urokinase has induced lysis of the experimental radioactive blood clot embolus in the circulation. Demonstration of effectiveness in this animal experiment is hampered by inhibition of the agents in the circulation to a degree much greater than was noted in previous experiments with streptokinase. In vitro testing indicates that under proper conditions urokinase will be an effective agent in the treatment of human thromboembolism.

Sign in / Sign up

Export Citation Format

Share Document