Macroscopic and histologic improvements in joint cartilage, subchondral bone and synovial membrane with glycosaminoglycans and native type ii collagen in a rabbit model of osteoarthritis

We sought to determine the cartilage repair capacity of WIN-34B in the collagenase-induced osteoarthritis rabbit model and in progenitor cells from subchondral bone. The cartilage protective effect of WIN-34B was measured by clinical and histological scores, cartilage area, and proteoglycan and collagen contents in the collagenase-induced osteoarthritis rabbit model. The efficacy of chondrogenic differentiation of WIN-34B was assessed by expression of CD105, CD73, type II collagen, and aggrecanin vivoand was analyzed by the surface markers of progenitor cells, the mRNA levels of chondrogenic marker genes, and the level of proteoglycan, GAG, and type II collagenin vitro. Oral administration of WIN-34B significantly increased cartilage area, and this was associated with the recovery of proteoglycan and collagen content. Moreover, WIN-34B at 200 mg/kg significantly increased the expression of CD105, CD73, type II collagen, and aggrecan compared to the vehicle group. WIN-34B markedly enhanced the chondrogenic differentiation of CD105 and type II collagen in the progenitor cells from subchondral bone. Also, we confirmed that treatment with WIN-34B strongly increased the number of SH-2(CD105) cells and expression type II collagen in subchondral progenitor cells. Moreover, WIN-34B significantly increased proteoglycan, as measured by alcian blue staining; the mRNA level of type IIα1 collagen, cartilage link protein, and aggrecan; and the inhibition of cartilage matrix molecules, such as GAG and type II collagen, in IL-1β-treated progenitor cells. These findings suggest that WIN-34B could be a potential candidate for effective anti-osteoarthritic therapy with cartilage repair as well as cartilage protection via enhancement of chondrogenic differentiation in the collagenase-induced osteoarthritis rabbit model and progenitor cells from subchondral bone.

Download Full-text

Healing of Circular Defects in the Rabbit Medial Meniscus Can Occur Spontaneously and Is not Improved by Intra-Articular Hyaluronic Acid

Veterinary and Comparative Orthopaedics and Traumatology ◽

10.1055/s-0038-1632504 ◽

1996 ◽

Vol 09 (02) ◽

pp. 60-5 ◽

Cited By ~ 9

Author(s):

N. Hope ◽

P. Ghosh ◽

S. Collier

Keyword(s):

Hyaluronic Acid ◽

Medial Meniscus ◽

Chondroitin Sulphate ◽

Rabbit Model ◽

Type Ii Collagen ◽

Type Ii ◽

Keratan Sulphate ◽

Meniscal Healing ◽

Meniscus Healing ◽

Made In

SummaryThe aim of this study was to determine the effects of intra-articular hyaluronic acid on meniscal healing. Circular defects, 1.0 mm in diameter, were made in the anterior third of the medial meniscus in rabbits. In one joint, 0.4 ml hyaluronic acid (Healon®) was instilled, and in the contralateral (control) joint, 0.4 ml Ringer’s saline. Four rabbits were killed after four, eight and 12 weeks and the menisci examined histologically. By eight weeks most of the lesions had healed by filling with hyaline-like cartilage. Healing was not improved by hyaluronic acid treatment. The repair tissue stained strongly with alcian blue, and the presence of type II collagen, keratan sulphate, and chondroitin sulphate was demonstrated by immunohistochemical localisation. In contrast to the circular defects, longitudinal incisions made in the medial menisci of a further six rabbits did not show any healing after 12 weeks, indicating that the shape of the lesion largely determined the potential for healing.The effect of hyaluronic acid on meniscal healing was tested in a rabbit model. With one millimeter circular lesions in the medial meniscus, healing by filling with hyalinelike cartilage was not significantly affected by the application of hyaluronic acid intra-articularly at the time of surgery, compared to saline controls, as assessed histologically four, eight and 12 weeks after the operation.

Download Full-text

Mixed Type I and Type II Collagen Scaffold for Cartilage Repair: Ultrastructural Study of Synovial Membrane Response and Healing Potential versus Microfractures (A Pilot Study)

International Journal of Immunopathology and Pharmacology ◽

10.1177/039463201302600410 ◽

2013 ◽

Vol 26 (4) ◽

pp. 917-930 ◽

Cited By ~ 3

Author(s):

D. Enea ◽

D. Guerra ◽

J. Roggiani ◽

S. Cecconi ◽

S. Manzotti ◽

...

Keyword(s):

Pilot Study ◽

Cartilage Repair ◽

Ultrastructural Study ◽

Synovial Membrane ◽

Mixed Type ◽

Potential Versus ◽

Collagen Scaffold ◽

Type Ii Collagen ◽

Type I ◽

Type Ii

Download Full-text

Morphological Assessment of MACI Grafts in Patients with Revision Surgery and Total Joint Arthroplasty

Cartilage ◽

10.1177/1947603519890754 ◽

2019 ◽

pp. 194760351989075 ◽

Cited By ~ 3

Author(s):

Aswin Beck ◽

David Wood ◽

Christopher J. Vertullo ◽

Jay Ebert ◽

Greg Janes ◽

...

Keyword(s):

Cartilage Repair ◽

Subchondral Bone ◽

Revision Surgery ◽

Total Joint Arthroplasty ◽

Type Ii Collagen ◽

Joint Arthroplasty ◽

Type I ◽

Type Ii ◽

Osteophyte Formation ◽

Repair Tissue

Objective To compare the histological and immunohistochemical characteristics of matrix-assisted chondrocyte implantation (MACI) grafts between patients with revision surgery and patients with total joint arthroplasty. Methods Biopsies of MACI grafts from patients with revision and total joint arthroplasty. The graft tissue characteristics and subchondral bone were examined by qualitative histology, ICRS (International Cartilage Repair Society) II scoring and semiquantitative immunohistochemistry using antibodies specific to type I and type II collagen. Results A total of 31 biopsies were available, 10 undergoing total knee arthroplasty (TKA) and 21 patients undergoing revision surgery. Patients in the clinically failed group were significantly older (46.3 years) than patients in the revision group (36.6 years) ( P = 0.007). Histologically, the predominant tissue in both groups was of fibrocartilaginous nature, although a higher percentage of specimens in the revision group contained a hyaline-like repair tissue. The percentages of type I collagen (52.9% and 61.0%) and type II collagen (66.3% and 42.2%) were not significantly different between clinically failed and revised MACI, respectively. The talar dome contained the best and patella the worst repair tissue. Subchondral bone pathology was present in all clinically failed patients and consisted of bone marrow lesions, including edema, necrosis and fibrosis, intralesional osteophyte formation, subchondral bone plate elevation, intralesional osteophyte formation, subchondral bone cyst formation, or combinations thereof. Conclusions MACI grafts in patients with revision and total joint arthroplasty were predominantly fibrocartilage in repair type, did not differ in composition and were histologically dissimilar to healthy cartilage. Clinically failed cases showed evidence of osteochondral unit failure, rather than merely cartilage repair tissue failure. The role of the subchondral bone in relation to pain and failure and the pathogenesis warrants further investigation.

Download Full-text

Absence of antibodies to native type I and type II collagen in a rabbit model of osteoarthritis

Arthritis & Rheumatism ◽

10.1002/anr.1780320629 ◽

1989 ◽

Vol 32 (6) ◽

pp. 814-815 ◽

Cited By ~ 4

Author(s):

Daniel J. Hartmann ◽

GuylèNe Charrière ◽

Gérard Ville ◽

Eric Vignon ◽

Jacques Bejui

Keyword(s):

Rabbit Model ◽

Type Ii Collagen ◽

Type I ◽

Type Ii

Download Full-text

Effects of Twin Inclined Plane Device on Adaptation and Ultrastructure Variations in Condyle of Growing Rats

BioMed Research International ◽

10.1155/2019/3069347 ◽

2019 ◽

Vol 2019 ◽

pp. 1-10

Author(s):

Shuo Wang ◽

Yuhong Sun ◽

Lulu Xia ◽

Hanyue Li ◽

Yan Xu ◽

...

Keyword(s):

Subchondral Bone ◽

Type Ii Collagen ◽

Ultrastructural Changes ◽

Control Group ◽

Tartrate Resistant Acid Phosphatase ◽

Type Ii ◽

Inclined Plane ◽

Growing Rats ◽

Osteoclastic Activity ◽

Experimental Group

Objective. This study investigates the effects of using a twin inclined plane device (TIPD) on the remolding and ultrastructure variation of mandibular condyle in growing rats. Materials and Methods. Forty-eight male Wistar rats (six weeks old, body weight of approximately 190–210 g) were divided into experimental group (wearing appliance, n = 32) and control group (no appliance, n = 16). Samples were collected on days 3, 14, 30, and 60. The immunohistochemical analysis for vascular endothelial growth factor (VEGF) and type II collagen was carried out. Tartrate-resistant acid phosphatase (TRAP) reaction was performed to evaluate the osteoclastic activity. Three-dimensional morphometric images were reconstructed for morphometric analysis by microcomputed tomography (micro-CT). The ultrastructure of the condylar surface was observed by scanning electron microscopy (SEM). Results. The expression of VEGF significantly increased, while the expression of type II collagen decreased in the experimental group at days 30 and 60. Furthermore, the enhanced osteoclast activity was observed under the subchondral bone, which was highest at day 30, and decreased to the lowest at day 60 in the experimental group. In addition, adaptive subchondral bone remolding in the posterior part of the condyle was observed at day 60 in the experimental group, and the SEM revealed the ultrastructure variations after installation of the TIPD. However, these changes began to reverse after 30 days. Conclusion. Condylar tissue changes point to the osteoclastic activity in the posterior region of the condyle. These adaptive changes point to bone resorption in the posterior condyle. Type II collagen and VEGF contribute to the MCC remolding induced by the TIPD. The ultrastructural changes in the posterior condylar area in response to mechanical stresses are recoverable at the initial stage.

Download Full-text