Platelet-rich Fibrin Increases Proliferation and Differentiation of Human Dental Pulp Cells

2010 ◽  
Vol 36 (10) ◽  
pp. 1628-1632 ◽  
Author(s):  
Fu-Mei Huang ◽  
Shun-Fa Yang ◽  
Jiing-Huei Zhao ◽  
Yu-Chao Chang
2021 ◽  
Vol 8 (4) ◽  
Author(s):  
Shihui Jiang ◽  
Zhaoxia Yu ◽  
Lanrui Zhang ◽  
Guanhua Wang ◽  
Xiaohua Dai ◽  
...  

Abstract This study aimed at evaluate the effects of different aperture-sized type I collagen/silk fibroin (CSF) scaffolds on the proliferation and differentiation of human dental pulp cells (HDPCs). The CSF scaffolds were designed with 3D mapping software Solidworks. Three different aperture-sized scaffolds (CSF1–CSF3) were prepared by low-temperature deposition 3D printing technology. The morphology was observed by scanning electron microscope (SEM) and optical coherence tomography. The porosity, hydrophilicity and mechanical capacity of the scaffold were detected, respectively. HDPCs (third passage, 1 × 105 cells) were seeded into each scaffold and investigated by SEM, CCK-8, alkaline phosphatase (ALP) activity and HE staining. The CSF scaffolds had porous structures with macropores and micropores. The macropore size of CSF1 to CSF3 was 421 ± 27 μm, 579 ± 36 μm and 707 ± 43 μm, respectively. The porosity was 69.8 ± 2.2%, 80.1 ± 2.8% and 86.5 ± 3.3%, respectively. All these scaffolds enhanced the adhesion and proliferation of HDPCs. The ALP activity in the CSF1 group was higher than that in the CSF3 groups (P < 0.01). HE staining showed HDPCs grew in multilayer within the scaffolds. CSF scaffolds significantly improved the adhesion and ALP activity of HDPCs. CSF scaffolds were promising candidates in dentine-pulp complex regeneration.


PLoS ONE ◽  
2013 ◽  
Vol 8 (12) ◽  
pp. e83545 ◽  
Author(s):  
Emilio Satoshi Hara ◽  
Mitsuaki Ono ◽  
Takanori Eguchi ◽  
Satoshi Kubota ◽  
Hai Thanh Pham ◽  
...  

2017 ◽  
Vol 83 ◽  
pp. 33-39 ◽  
Author(s):  
Qin Liu ◽  
Wenguo Fan ◽  
Yifan He ◽  
Fuping Zhang ◽  
Xiaoyan Guan ◽  
...  

2014 ◽  
Vol 40 (10) ◽  
pp. 1593-1599 ◽  
Author(s):  
Zhichun Zhang ◽  
Qingru Guo ◽  
Hua Tian ◽  
Ping Lv ◽  
Chunyan Zhou ◽  
...  

2016 ◽  
Vol 42 (11) ◽  
pp. 1633-1640 ◽  
Author(s):  
Xuan He ◽  
Wen-Xia Chen ◽  
Guifei Ban ◽  
Wei Wei ◽  
Jun Zhou ◽  
...  

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