An LC–MS/MS method for the simultaneous determination of chlorogenic acid, forsythiaside A and baicalin in rat plasma and its application to pharmacokinetic study of Shuang-huang-lian in rats

2010 ◽  
Vol 52 (4) ◽  
pp. 625-630 ◽  
Author(s):  
Jun-Xiao Ye ◽  
Wang Wei ◽  
Li-Hui Quan ◽  
Chun-Yu Liu ◽  
Qi Chang ◽  
...  
Keyword(s):  
Chlorogenic Acid ◽  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Rat Plasma

scholarly journals Simultaneous Determination of Chlorogenic Acid Isomers and Metabolites in Rat Plasma Using LC-MS/MS and Its Application to A Pharmacokinetic Study Following Oral Administration of Stauntonia Hexaphylla Leaf Extract (YRA-1909) to Rats

Pharmaceutics ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 143 ◽  
Author(s):  
Won-Gu Choi ◽  
Ju-Hyun Kim ◽  
Dong Kim ◽  
Yongnam Lee ◽  
Ji Yoo ◽  
...  
Keyword(s):  
Oral Administration ◽  
Chlorogenic Acid ◽  
Simultaneous Determination ◽  
Caffeic Acid ◽  
Pharmacokinetic Study ◽  
Leaf Extract ◽  
Rat Plasma ◽  
Mass Spectrometric Method ◽  
Tandem Mass Spectrometric

Stauntonia hexaphylla leaf extract (YRA-1909), which is widely used for the antirheumatic properties, has been under phase 2 clinical trials in patients with rheumatoid arthritis since April 2017. Liquid chromatography-tandem mass spectrometric method while using liquid–liquid extraction with ethyl acetate was validated for the simultaneous determination of the major active components of YRA-1909, including chlorogenic acid (CGA), neochlorogenic acid (NCGA), cryptochlorogenic acid (CCGA), and their metabolites (i.e., caffeic acid (CA), caffeic acid 3-O-glucuronide (CA-3-G), caffeic acid 4-O-glucuronide (CA-4-G), and ferulic acid (FA)) in rat plasma and applied to a pharmacokinetic study of YRA-1909 in rats. Seven analytes were separated on Halo C18 while using gradient elution of formic acid and methanol, and then quantified in selected reaction monitoring mode whle using negative electrospray ionization. Following oral administration of YRA-1909 at doses of 25, 50, and 100 mg/kg to male Sprague-Dawley rats, CGA, NCGA, and CCGA were rapidly absorbed and metabolized to CA, CA-3-G, and CA-4-G. The area under the plasma concentration-time curve (AUClast) of CGA, NCGA, CCGA, and three metabolites linearly increased as the YRA-1909 dose increased. Other pharmacokinetic parameters were comparable among three doses studied. AUClast values for CA, CA-3-G, and CA-4-G exceeded those for CGA, NCGA, and CCGA.

scholarly journals Simultaneous Determination and Pharmacokinetic Comparisons of Multi-Ingredients after Oral Administration ofRadix Salviae MiltiorrhizaeExtract, Hawthorn Extract, and a Combination of Both Extracts to Rats

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Yu-Qiang Liu ◽  
Qian Cai ◽  
Chang Liu ◽  
Feng-Wei Bao ◽  
Zhen-Qiu Zhang
Keyword(s):  
Oral Administration ◽  
Simultaneous Determination ◽  
Pharmacokinetic Study ◽  
Rosmarinic Acid ◽  
Rat Plasma ◽  
Hplc Method ◽  
Radix Salviae Miltiorrhizae ◽  
Salviae Miltiorrhizae ◽  
Hawthorn Extract

A simple and sensitive HPLC method was developed for simultaneous determination of danshensu (DSS), rosmarinic acid (RA), lithospermic acid (LA), salvianolic acid B (SAB), and hyperoside (HP) in rat plasma. This method validated was successfully applied to the pharmacokinetic study of the main active ingredients after oral administration ofRadix Salviae Miltiorrhizaeextract (SME), hawthorn extract (HTE), and a combination of both extracts (2.5 : 1) to rats. The results indicated that there have been great differences in pharmacokinetics between a single extract and a combination of both extracts. A combination of both extracts can enhance their bioavailabilities and delay the elimination of SAB and DSS in rats.

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