The membrane permeability of Astragali Radix-derived formononetin and calycosin is increased by Angelicae Sinensis Radix in Caco-2 cells: A synergistic action of an ancient herbal decoction Danggui Buxue Tang

2012 ◽  
Vol 70 ◽  
pp. 671-679 ◽  
Author(s):  
Ken Yu-zhong Zheng ◽  
Roy Chi-yan Choi ◽  
Ava Jiang-yang Guo ◽  
Cathy Wen-chuan Bi ◽  
Kevin Yue Zhu ◽  
...  
Keyword(s):  
Membrane Permeability ◽  
Synergistic Action ◽  
Astragali Radix ◽  
Danggui Buxue Tang ◽  
Herbal Decoction ◽  
Angelicae Sinensis

scholarly journals Functions of Danggui Buxue Tang, a Chinese Herbal Decoction Containing Astragali Radix and Angelicae Sinensis Radix, in Uterus and Liver are Both Estrogen Receptor-Dependent and -Independent

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Oliver Zierau ◽  
Ken Y. Z. Zheng ◽  
Anja Papke ◽  
Tina T. X. Dong ◽  
Karl W. K. Tsim ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Ovariectomized Rats ◽  
Transcript Expression ◽  
Astragali Radix ◽  
Danggui Buxue Tang ◽  
Chinese Herbal ◽  
Herbal Decoction ◽  
Estrogenic Properties ◽  
Angelicae Sinensis

Danggui Buxue Tang (DBT), a herbal decoction containing Astragali Radix (AR) and Angelicae Sinensis Radix (ASR), has been used in treating menopausal irregularity in women for more than 800 years in China. Pharmacological results showed that DBT exhibited significant estrogenic propertiesin vitro, which therefore suggested that DBT could activate the nuclear estrogen receptors. Here, we assessed the estrogenic properties of DBT in an ovariectomizedin vivorat model: DBT was applied to the ovariectomized rats for 3 days. The application of DBT did not alter the weight of uterus and liver, as well as the transcript expression of the proliferation markers including the estrogen receptorsαandβ. However, DBT stimulated the transcript expression of the estrogen responsive genes. In addition, the inductive role of DBT on the expression of members of the aryl hydrocarbon receptor family in uterus and liver of ovariectomized rats was confirmed. These responses of DBT however were clearly distinct from the response pattern detectable here for 17β-estradiol. Therefore, DBT exhibited weak, but significant, estrogenic propertiesin vivo; however, some of its activities were independent of the estrogen receptor. Thus, DBT could be an exciting Chinese herbal decoction for an alternative treatment of hormone replacement therapy for women in menopause without subsequent estrogenic side effects.

scholarly journals The Osteogenic Function of Danggui Buxue Tang, a Herbal Decoction Containing Astragali Radix and Angelicae Sinensis Radix, Is Optimized by Boiling the Two Herbs Together: Signaling Analyses Revealed by Systems Biology

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1119
Author(s):  
Kenneth K L Kwan ◽  
Tin Yan Wong ◽  
Anna X. D. Yu ◽  
Tina T. X. Dong ◽  
Henry H. L. Lam ◽  
...  
Keyword(s):  
Systems Biology ◽  
Herbal Extract ◽  
Complex Biological System ◽  
Astragali Radix ◽  
Danggui Buxue Tang ◽  
Herbal Mixture ◽  
Herbal Decoction ◽  
Cellular Pathways ◽  
Angelicae Sinensis ◽  
Rat Osteoblasts

The therapeutic efficacy of a herbal mixture, being multi-target, multi-function and multi-pathway, is the niche of traditional Chinese medicine (TCM). Systems biology can dissect the network of signaling mechanisms in a complex biological system. In preparing TCM decoctions, the boiling of herbs together in water is a common practice; however, the rationale of this specific preparation has not been fully revealed. An approach of mass-spectrometry-based multi-omics was employed to examine the profiles of the cellular pathways, so as to understand the pharmacological efficacy of Danggui Buxue Tang (DBT), a Chinese herbal mixture containing Astragali Radix and Angelicae Sinensis Radix, in cultured rat osteoblasts and mesenchymal stem cells. The results, generated from omics analyses, were compared from DBT-treated osteoblasts to those of treating the herbal extract by simple mixing of extracts from Astragali Radix and Angelicae Sinensis Radix, i.e., herbal mixture without boiling together. The signaling pathways responsible for energy metabolism and amino acid metabolism showed distinct activation, as triggered by DBT, in contrast to simple mixing of two herbal extracts. The result supports that boiling the herbs together is designed to maximize the osteoblastic function of DBT, such as in energy and lipid metabolism. This harmony of TCM formulation, by having interactive functions of two herbs during preparation, is being illustrated. The systems biology approach provides new and essential insights into the synergy of herbal preparation. Well-defined multiple targets and multiple pathways in different levels of omics are the key to modernizing TCM.

scholarly journals A Chinese Herbal Decoction, Danggui Buxue Tang, Stimulates Proliferation, Differentiation and Gene Expression of Cultured Osteosarcoma Cells: Genomic Approach to Reveal Specific Gene Activation

2011 ◽  
Vol 2011 ◽  
pp. 1-13 ◽  
Author(s):  
Roy C. Y. Choi ◽  
Qiu T. Gao ◽  
Anna W. H. Cheung ◽  
Judy T. T. Zhu ◽  
Faye T. C. Lau ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Activation ◽  
Specific Gene ◽  
Danggui Buxue Tang ◽  
Radix Angelicae Sinensis ◽  
Chinese Herbal ◽  
Cell Proliferation And Differentiation ◽  
Herbal Decoction ◽  
Regulated Gene Expression ◽  
Angelicae Sinensis

Danggui Buxue Tang (DBT), a Chinese herbal decoction used to treat ailments in women, contains Radix Astragali (Huangqi; RA) and Radix Angelicae Sinensis (Danggui; RAS). When DBT was applied onto cultured MG-63 cells, an increase of cell proliferation and differentiation of MG-63 cell were revealed: both of these effects were significantly higher in DBT than RA or RAS extract. To search for the biological markers that are specifically regulated by DBT, DNA microarray was used to reveal the gene expression profiling of DBT in MG-63 cells as compared to that of RA- or RAS-treated cells. Amongst 883 DBT-regulated genes, 403 of them are specifically regulated by DBT treatment, including CCL-2, CCL-7, CCL-8, and galectin-9. The signaling cascade of this DBT-regulated gene expression was also elucidated in cultured MG-63 cells. The current results reveal the potential usage of this herbal decoction in treating osteoporosis and suggest the uniqueness of Chinese herbal decoction that requires a well-defined formulation. The DBT-regulated genes in the culture could serve as biological responsive markers for quality assurance of the herbal preparation.

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