Air assisted dispersive liquid-liquid microextraction with solidification of the floating organic droplets (AA-DLLME-SFO) and UHPLC-PDA method: Application to antibiotics analysis in human plasma of hospital acquired pneumonia patients

2018 ◽  
Vol 151 ◽  
pp. 266-273 ◽  
Author(s):  
Vincenzo Ferrone ◽  
Roberto Cotellese ◽  
Maura Carlucci ◽  
Lorenzo Di Marco ◽  
Giuseppe Carlucci
Keyword(s):  
Human Plasma ◽  
Hospital Acquired Pneumonia ◽  
Dispersive Liquid Liquid Microextraction ◽  
Hospital Acquired ◽  
Liquid Microextraction

Vortex-Assisted Dispersive Liquid–Liquid Microextraction Coupled with Deproteinization for Determination of Nateglinide in Human Plasma Using HPLC/UV

2020 ◽  
Author(s):  
Mohamed A Hammad ◽  
Amira H Kamal ◽  
Reham E Kannouma ◽  
Fotouh R Mansour
Keyword(s):  
Human Plasma ◽  
High Performance ◽  
Limit Of Detection ◽  
Signal To Noise Ratio ◽  
Cost Effective ◽  
Salt Addition ◽  
Relative Standard ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

Abstract A validated method for preconcentration and determination of nateglinide in plasma was developed using vortex-assisted dispersive liquid–liquid microextraction. Different variables that affect extraction efficiency were studied and optimized, including type and volume of extractant, type and volume of disperser, pH of diluent, salt addition effect, centrifugation and vortex time. Nateglinide was extracted using 30 μL of 1-octanol as an extractant and 200 μL of methanol as a disperser. The enrichment factor reached 330 under the optimum conditions. High-performance liquid chromatography/ultraviolet was used for detection using phosphate buffer (pH 2.5, 10 mM): acetonitrile (45:55, v/v) as a mobile phase at a flow rate of 1 mL/min. The method was linear over the range of 50–20,000 ng/mL with a limit of detection of 15 ng/mL (signal-to-noise ratio = 3). Intra- and inter-day precision had %relative standard deviation <6% (n = 3) and the %recoveries were found to be between 102.5 and 105.9%. The proposed method is simple, sensitive, eco-friendly, cost-effective and powerful for microextraction of nateglinide from human plasma samples.

scholarly journals Development of an ionic liquid-based dispersive liquid–liquid microextraction method for the determination of nifurtimox and benznidazole in human plasma

Talanta ◽  
2013 ◽  
Vol 107 ◽  
pp. 95-102 ◽  
Author(s):  
J.M. Padró ◽  
M.E. Marsón ◽  
G.E. Mastrantonio ◽  
J. Altcheh ◽  
F. García-Bournissen ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Human Plasma ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction
Sign in / Sign up

Export Citation Format

Share Document