scholarly journals Vicenin-2 ameliorates oxidative damage and photoaging via modulation of MAPKs and MMPs signaling in UVB radiation exposed human skin cells

2019 ◽  
Vol 190 ◽  
pp. 76-85 ◽  
Author(s):  
Xi Duan ◽  
Tao Wu ◽  
Ting Liu ◽  
Hao Yang ◽  
Xiaojie Ding ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Human Skin ◽  
Uvb Radiation ◽  
Skin Cells ◽  
Human Skin Cells

scholarly journals New functions of XPC in the protection of human skin cells from oxidative damage

2006 ◽  
Vol 25 (18) ◽  
pp. 4305-4315 ◽  
Author(s):  
Mariarosaria D'Errico ◽  
Eleonora Parlanti ◽  
Massimo Teson ◽  
Bruno M Bernardes de Jesus ◽  
Paolo Degan ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Human Skin ◽  
Skin Cells ◽  
Human Skin Cells

In VitroStudy of the Stress Response of Human Skin Cells to GSM-1800 Mobile Phone Signals Compared to UVB Radiation and Heat Shock

2007 ◽  
Vol 167 (5) ◽  
pp. 572-580 ◽  
Author(s):  
S. Sanchez ◽  
E. Haro ◽  
G. Ruffié ◽  
B. Veyret ◽  
I. Lagroye
Keyword(s):  
Stress Response ◽  
Heat Shock ◽  
Mobile Phone ◽  
Human Skin ◽  
Uvb Radiation ◽  
Skin Cells ◽  
Human Skin Cells

scholarly journals Differential expression of selenoproteins by human skin cells and protection by selenium from UVB-radiation-induced cell death

1998 ◽  
Vol 332 (1) ◽  
pp. 231-236 ◽  
Author(s):  
Teresa S. RAFFERTY ◽  
Roderick C. McKENZIE ◽  
John A. A. HUNTER ◽  
A. Forbes HOWIE ◽  
John R. ARTHUR ◽  
...  
Keyword(s):  
Cell Death ◽  
Human Skin ◽  
Sodium Selenite ◽  
Human Fibroblasts ◽  
Human Keratinocytes ◽  
Uvb Radiation ◽  
Skin Damage ◽  
Skin Cells ◽  
Radiation Induced ◽  
Human Skin Cells

The generation of reactive oxygen species has been implicated as part of the mechanism responsible for UVB-radiation-induced skin damage. In mice, evidence suggests that increased dietary selenium intake may protect skin from many of the harmful effects of UVB radiation. We sought to determine the selenoprotein profile of cultured human skin cells and whether selenium supplementation could protect keratinocytes and melanocytes from the lethal effects of UVB radiation. Labelling experiments using [75Se]selenite showed qualitative and quantitative differences in selenoprotein expression by human fibroblasts, keratinocytes and melanocytes. This was most noticeable for thioredoxin reductase (60 kDa) and phospholipid glutathione peroxidase (21 kDa); these proteins were identified by Western blotting. Despite these differences, we found that a 24 h preincubation with sodium selenite or selenomethionine protected both cultured human keratinocytes and melanocytes from UVB-induced cell death. With primary keratinocytes, the greatest reduction in cell death was found with 10 nM sodium selenite (79% cell death reduced to 21.7%; P< 0.01) and with 50 nM selenomethionine (79% cell death reduced to 13.2%; P< 0.01). Protection could be obtained with concentrations as low as 1 nM with sodium selenite and 10 nM with selenomethionine. When selenium was added after UVB radiation, little protection could be achieved, with cell death only being reduced from 88.5% to about 50% with both compounds. In all of the experiments sodium selenite was more potent than selenomethionine at providing protection from UVB radiation.

scholarly journals Exogenous Antioxidants Impact on UV-Induced Changes in Membrane Phospholipids and the Effectiveness of the Endocannabinoid System in Human Skin Cells

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1260
Author(s):  
Agnieszka Gęgotek ◽  
Anna Jastrząb ◽  
Marta Dobrzyńska ◽  
Michał Biernacki ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Ascorbic Acid ◽  
Cell Viability ◽  
Human Skin ◽  
Phospholipid Fatty Acid ◽  
Antioxidant Properties ◽  
Endocannabinoid System ◽  
Membrane Phospholipids ◽  
Skin Cells ◽  
Human Skin Cells ◽  
Induced Changes

Natural antioxidants effectively counteract changes caused by UV radiation in human skin cells. However, their action is limited due to their lipo/hydrophilicity. Therefore, the aim of this study was to analyze the mutual protective action of hydrophilic ascorbic acid and partially lipophilic rutin against UVA/UVB-induced changes in membranes phospholipid and endocannabinoid system in keratinocytes and fibroblasts. Obtained results clearly showed that, despite the stronger antioxidant properties of ascorbic acid, the lipid membranes were more effectively protected against UV-induced oxidation by rutin, including changes in phospholipid fatty acid levels, prevention against reactive aldehydes formation and endocannabinoids degradation. Ascorbic acid more strongly prevented UV-induced endocannabinoid receptors expression in fibroblasts, especially CB1. However, the combined action of used antioxidants resulted in the greatest cytoprotective effect, which was evident in the inflammatory marker TNFα down-regulation and increased cell viability following cell irradiation. The applied mixture of antioxidants showed a stronger protective in relation to membrane phospholipids in keratinocytes and in the endocannabinoid system in fibroblasts. In conclusion, it can be suggested that combined antioxidant capacities of ascorbic acid and rutin protects against lipid peroxidation but also decreases the UV-induced inflammation by direct interaction with the endocannabinoid system, thus increasing skin cell viability.

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