Abscisic acid is involved in brassinosteroids-induced chilling tolerance in the suspension cultured cells from Chorispora bungeana

2011 ◽  
Vol 168 (9) ◽  
pp. 853-862 ◽  
Author(s):  
Yajie Liu ◽  
Haifeng Jiang ◽  
Zhiguang Zhao ◽  
Lizhe An
Keyword(s):  
Abscisic Acid ◽  
Cultured Cells ◽  
Chilling Tolerance ◽  
Suspension Cultured Cells ◽  
Chorispora Bungeana

scholarly journals Phytohormones Regulate the Non-Redundant Response of ω-3 Fatty Acid Desaturases to Low Temperatures in Chorispora Bungeana

2021 ◽  
Author(s):  
Yulan Shi ◽  
Sizhong Yang ◽  
Xiule Yue ◽  
Zhixing Zhao ◽  
Lizhe An
Keyword(s):  
Fatty Acids ◽  
Abscisic Acid ◽  
Fatty Acid ◽  
Stress Response ◽  
Low Temperatures ◽  
Cultured Cells ◽  
Temperature Decrease ◽  
Fatty Acid Desaturases ◽  
Cold Environments ◽  
Chorispora Bungeana

Abstract To explore the contribution of ω-3 fatty acid desaturases (FADs) to cold stress response in a special cryophyte, Chorispora bungeana (C. bungeana), two plastidial ω-3 FAD genes (CbFAD7 and CbFAD8) were cloned and verified in a Arabidopsis fad7fad8 mutant, before being compared with the microsomal ω-3 FAD gene (CbFAD3) on expression profile. Though these genes were expressed in all tested tissues of C. bungeana, CbFAD7 and CbFAD8 have the highest expression in leaves, while CbFAD3 was mostly expressed in non-green tissues. Low temperatures (4, 0 and -4 ℃) resulted in significant increases in trienoic fatty acids (TAs, mainly C18:3), which were consistent with the non-redundant expression of CbFAD3 and CbFAD8 in suspension-cultured cells, and the coordination of CbFAD7 and CbFAD8 in leaves. Furthermore, the contribution of CbFAD8 increased as temperature decrease in the two tissues. Our data revealed that jasmonie acid and brassinosteroids participated in the cold-responsive expression of these genes in both tissues, and the pyhtohormone regulation in leaves was more complicated with the participation of abscisic acid and gibberellin. These results point to the hormone-regulated non-redundant contribution of ω-3 CbFADs to maintain appropriate level of TAs under low temperatures, which help C. bungeana survive in cold environments.

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