Comparison of the effects of intermittent reperfusion and hypothermia in preventing testicular ischemia-reperfusion injury in the testicular torsion model in rats

Abstract Background Testicular torsion is a urological emergency in which misdiagnosis and inappropriate treatment can lead to testicular atrophy and male infertility owing to ischemia-reperfusion injury (IRI). Although experimental studies of testicular torsion have been preceded, promising therapeutic agents based on the long-term effect for spermatogenesis have not been identified in testicular ischemia reperfusion injury (IRI) animal model. Tadalafil, one of the phosphodiesterase-5 inhibitors commonly used in the treatment of erectile dysfunction, has recently reported a protective effect against IRI in several organs. In this study, we evaluated the long-term protective effect of tadalafil for spermatogenesis in a rat testicular IRI model. Methods Forty-eight adolescent Sprague–Dawley rats were divided into 6 groups (A-F). Sham operation was performed in group A. Group B received surgical 720-degree torsion of the left testis without any medication. Groups C, D, E, and F were operated surgical torsion with tadalafil at varying doses (0.3 mg/kg, 1.0 mg/kg) and durations (single or daily administration for 4 weeks). Detorsion was performed after 3 hour of torsion in all rats except the sham group. Four weeks after operation, both testes were evaluated of spermatogenesis using Johnsen scoring. To evaluate the protective effect of tadalafil against oxidative stress by IRI, malondialdehyde (MDA) and superoxide dismutase (SOD) level were analyzed via ELISA in both testes 4 hour after detorsion in the same experiments as in group A, B, and C. Results For the evaluation of spermatogenesis according to doses, the groups with high-dose tadalafil showed a higher Johnsen scores than low-dose counterparts. The groups with daily administration for 4weeks were observed a higher Johnsen scores than those given a single administration. Furthermore, molecular markers (MDA and SOD) related with oxidative stress and histopathologic findings showed remarkable improvement after tadalafil administration. Conclusion Tadalafil alleviated long-term deterioration of spermatogenesis and oxidative stress by restoring antioxidant status after testicular IRI rat model. Furthermore, it demonstrated a protective effect against testicular IRI in a time- and dose-dependent manner.

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Effects of melatonin on spermatogenesis and testicular ischemia-reperfusion injury after unilateral testicular torsion-detorsion

Journal of Pediatric Surgery ◽

10.1016/j.jpedsurg.2008.01.065 ◽

2008 ◽

Vol 43 (10) ◽

pp. 1873-1878 ◽

Cited By ~ 28

Author(s):

Müslim Yurtçu ◽

Adnan Abasiyanik ◽

Mustafa Cihat Avunduk ◽

Sabahattin Muhtaroğlu

Keyword(s):

Reperfusion Injury ◽

Testicular Torsion ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Testicular Ischemia

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Protective Effects of Topical Application of Nitrite on Testicular Ischemia-Reperfusion Injury in Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2021/5514537 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Jae Won Lee ◽

Ee Taek Hwang ◽

Jin Soo Han

Keyword(s):

Reperfusion Injury ◽

Topical Application ◽

Testicular Torsion ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Therapeutic Effects ◽

Treatment Groups ◽

Group A ◽

Testicular Ischemia ◽

Group B

Testicular torsion is a urologic emergency induced by torsion of the spermatic cord, interrupting blood circulation to the testis. Therapeutic options for testicular torsion, except surgical restoration of testis, are rarely applied in clinical practice. This study, therefore, investigated whether topical application of nitrite (NO2-) is beneficial in tissue damage due to testicular ischemia-reperfusion (I/R) injury in rats. Pubertal Sprague-Dawley rats were assigned to seven groups: group A, sham-operated control group; group B, I/R with no treatment; groups C, D, and E, I/R followed by treatment with three different doses of nitrite; group F, I/R followed by administration of nitrite and a NO scavenger, C-PTIO (2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium salt); and group G, I/R followed by administration of nitrate (NO3-). Unilateral testicular ischemia was maintained for 5 h, followed by reperfusion for 24 h. Nitrite and nitrate were topically administered before reperfusion. Compared to group A, germ cell apoptosis, oxidative stress, antioxidant enzymatic function, and lipid peroxidation were significantly increased, along with abnormal morphology and impaired spermatogenesis in group B ( P < 0.05 ). In contrast, testicular damage was generally attenuated in the nitrite treatment groups due to a reduction in superoxide and peroxynitrite levels and the inhibition of caspase-3-dependent apoptosis ( P < 0.05 vs. group B). These therapeutic effects of nitrite-derived NO were suppressed after injection of C-PTIO, which showed in group F. Taken together, our results demonstrate that topical application of nitrite may be one of the therapeutic strategies for testicular ischemia-reperfusion injury.

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Sesamol Protects Testis from Ischemia-Reperfusion Injury through Scavenging Reactive Oxygen Species and Upregulating CREMτ Expression

Oxidative Medicine and Cellular Longevity ◽

10.1155/2020/9043806 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Si-Ming Wei ◽

Rong-Yun Wang ◽

Yan-Song Chen

Keyword(s):

Reactive Oxygen Species ◽

Reperfusion Injury ◽

Testicular Torsion ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Reactive Oxygen ◽

Control Group ◽

Oxygen Species ◽

Germ Cell Differentiation ◽

Testicular Ischemia

Testicular torsion/detorsion-induced damage is considered as a typical ischemia-reperfusion injury attributed to excessive reactive oxygen species (ROS) production. ROS may regulate many genes whose expression affects cell-cycle regulation, cell proliferation, and apoptosis. The cAMP-responsive element modulator-τ (CREMτ) gene expression in the testis is essential for normal germ cell differentiation. The present study was aimed at investigating the effect of sesamol, a powerful antioxidant, on testicular ischemia-reperfusion injury and related mechanisms in an experimental testicular torsion-detorsion rat model. The type of our study was a randomized controlled trial. Sixty rats were randomly divided into the following 3 groups: (1) sham-operated control group (n=20), (2) testicular ischemia-reperfusion group (n=20), and (3) testicular ischemia-reperfusion+sesamol-treated group (n=20). Testicular ischemia-reperfusion was induced by left testicular torsion (720° rotation in a counterclockwise direction) for 2 hours, followed by detorsion. Orchiectomy was performed at 4 hours or 3 months after detorsion. The testis was obtained for the analysis of the following parameters, including malondialdehyde level (a sensitive indicator of ROS), CREMτ expression, and spermatogenesis. In the testicular ischemia-reperfusion group, the malondialdehyde level was significantly increased with a concomitant significant decrease in CREMτ expression and spermatogenesis in ipsilateral testis. These results suggest that overproduction of ROS after testicular ischemia-reperfusion may downregulate CREMτ expression, which causes spermatogenic injury. Sesamol treatment resulted in a significant reduction in the malondialdehyde level and significant increase in CREMτ expression and spermatogenesis in ipsilateral testis. These data support the above suggestion. Our study shows that sesamol can attenuate testicular ischemia-reperfusion injury through scavenging ROS and upregulating CREMτ expression.

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Protective effect of Bmscs-derived exosomes on testicular ischemia-reperfusion injury in rats

10.21203/rs.3.rs-184044/v3 ◽

2021 ◽

Author(s):

Hu Tian ◽

Wan-song Zhang ◽

Cheng Yang ◽

Jun-hao Zhou ◽

Ran-ran Zhou ◽

...

Keyword(s):

Oxidative Stress ◽

Bone Marrow ◽

Stem Cell ◽

Treatment Group ◽

Mesenchymal Stem Cell ◽

Reperfusion Injury ◽

Testicular Torsion ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Testicular Ischemia

Abstract Background: Testicular Ischemia reperfusion injury(IRI) is a major pathophysiological process of surgical reduction after testicular torsion, and oxidative stress is the main injury factor. However, the role of BMSCs-derived exosomes in testicular IRI and its mechanism have not been reported. In this study, we investigated the protective effect of bone marrow mesenchymal stem cell-derived exosomes against testicular ischemia-reperfusion injury.Results: BMSCs were successfully isolated and cultured from rat bone marrow, and exosomes secreted by BMSCs were successfully extracted. In vivo experiment: The testicular torsion rat model was established, and various biochemical indexes of oxidative stress and testicular tissue HE was detected in the sham operation group, testicular torsion group and bone marrow mesenchymal stem cell-derived exosome treatment group. In vitro experiment: H2O2 was used to construct TM4 and GC1 oxidative stress models, and various biochemical indexes of oxidative stress and corresponding pathway proteins were detected in the control group, H2O2 group and bone marrow mesenchymal stem cell-derived exosome treatment group.Conclusion: BMSCs-derived exosomes can be absorbed by rat spermatogonia and have antioxidant and anti-inflammatory protective effects against testicular ischemia-reperfusion injury。

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Protective effect of Bmscs-derived exosomes on testicular ischemia-reperfusion injury in rats

10.21203/rs.3.rs-184044/v2 ◽

2021 ◽

Author(s):

Hu Tian ◽

Wan-song Zhang ◽

Cheng Yang ◽

Jun-hao Zhou ◽

Ran-ran Zhou ◽

...

Keyword(s):

Oxidative Stress ◽

Bone Marrow ◽

Stem Cell ◽

Treatment Group ◽

Mesenchymal Stem Cell ◽

Reperfusion Injury ◽

Testicular Torsion ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Testicular Ischemia

Abstract Background Testicular Ischemia reperfusion injury(IRI) is a major pathophysiological process of surgical reduction after testicular torsion, and oxidative stress is the main injury factor. However, the role of BMSCs-derived exosomes in testicular IRI and its mechanism have not been reported. In this study, we investigated the protective effect of bone marrow mesenchymal stem cell-derived exosomes against testicular ischemia-reperfusion injury. Methods Results: BMSCs were successfully isolated and cultured from rat bone marrow, and exosomes secreted by BMSCs were successfully extracted. In vivo experiment: The testicular torsion rat model was established, and various biochemical indexes of oxidative stress and testicular tissue HE was detected in the sham operation group, testicular torsion group and bone marrow mesenchymal stem cell-derived exosome treatment group. In vitro experiment: H2O2 was used to construct TM4 and GC1 oxidative stress models, and various biochemical indexes of oxidative stress and corresponding pathway proteins were detected in the control group, H2O2 group and bone marrow mesenchymal stem cell-derived exosome treatment group. Conclusion BMSCs-derived exosomes can be absorbed by rat spermatogonia and have antioxidant and anti-inflammatory protective effects against testicular ischemia-reperfusion injury。

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The Effect of L-Carnitine on Testicular Ischemia- Reperfusion Injury due to Testicular Torsion in Rats

Electronic Journal of General Medicine ◽

10.29333/ejgm/82460 ◽

2012 ◽

Vol 9 (3) ◽

Author(s):

Abdullah Gedik ◽

Hasan Deliktaş ◽

Yusuf Nergiz ◽

Mehmet Kamuran Bircan

Keyword(s):

Reperfusion Injury ◽

Testicular Torsion ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Testicular Ischemia

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Protective effect of Bmscs-derived exosomes on testicular ischemia-reperfusion injury in rats

10.21203/rs.3.rs-227211/v1 ◽

2021 ◽

Author(s):

Hu Tian ◽

Wan-song Zhang ◽

Cheng Yang ◽

Jun-hao Zhou ◽

Ran-ran Zhou ◽

...

Keyword(s):

Oxidative Stress ◽

Bone Marrow ◽

Stem Cell ◽

Treatment Group ◽

Mesenchymal Stem Cell ◽

Reperfusion Injury ◽

Testicular Torsion ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Testicular Ischemia

Abstract Background Testicular Ischemia reperfusion injury(IRI) is a major pathophysiological process of surgical reduction after testicular torsion, and oxidative stress is the main injury factor. However, the role of BMSCs-derived exosomes in testicular IRI and its mechanism have not been reported. In this study, we investigated the protective effect of bone marrow mesenchymal stem cell-derived exosomes against testicular ischemia-reperfusion injury. Methods Results: BMSCs were successfully isolated and cultured from rat bone marrow, and exosomes secreted by BMSCs were successfully extracted. In vivo experiment: The testicular torsion rat model was established, and various biochemical indexes of oxidative stress and testicular tissue HE was detected in the sham operation group, testicular torsion group and bone marrow mesenchymal stem cell-derived exosome treatment group. In vitro experiment: H2O2 was used to construct TM4 and GC1 oxidative stress models, and various biochemical indexes of oxidative stress and corresponding pathway proteins were detected in the control group, H2O2 group and bone marrow mesenchymal stem cell-derived exosome treatment group. Conclusion BMSCs-derived exosomes can be absorbed by rat spermatogonia and have antioxidant and anti-inflammatory protective effects against testicular ischemia-reperfusion injury。

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