scholarly journals Algorithmic robustness to preferred orientations in Single Particle Analysis by CryoEM

2021 ◽  
pp. 107695
Author(s):  
C.O.S. Sorzano ◽  
D. Semchonok ◽  
S.-C. Lin ◽  
Y.-C. Lo ◽  
J.L. Vilas ◽  
...  
Keyword(s):  
Single Particle ◽  
Particle Analysis ◽  
Single Particle Analysis

scholarly journals Thermo Scientific™ Tundra Cryo-TEM: 100kV Cryo-TEM dedicated for Single Particle Analysis

2021 ◽  
Vol 27 (S1) ◽  
pp. 1330-1332
Author(s):  
Zuzana Hlavenková ◽  
Dimple Karia ◽  
Miloš Malínský ◽  
Daniel Němeček ◽  
Fanis Grollios ◽  
...  
Keyword(s):  
Single Particle ◽  
Particle Analysis ◽  
Single Particle Analysis

SINGLE PARTICLE ANALYSIS OF FIXED FOG DROPLETS USING SR X-RAY MICROPROBE SYSTEM

2001 ◽  
Vol 32 ◽  
pp. 873-874
Author(s):  
S. TOHNO ◽  
S. HAYAKAWA ◽  
A. NAKAMURA ◽  
A. HAMAMOTO ◽  
M. SUZUKI ◽  
...  
Keyword(s):  
Single Particle ◽  
Particle Analysis ◽  
Single Particle Analysis ◽  
X Ray

scholarly journals Benchmarking cryo-EM Single Particle Analysis Workflow

2018 ◽  
Vol 5 ◽  
Author(s):  
Laura Y. Kim ◽  
William J. Rice ◽  
Edward T. Eng ◽  
Mykhailo Kopylov ◽  
Anchi Cheng ◽  
...  
Keyword(s):  
Single Particle ◽  
Particle Analysis ◽  
Single Particle Analysis ◽  
Analysis Workflow

Inside versus Outside: Ion Redistribution in Nitric Acid Reacted Sea Spray Aerosol Particles as Determined by Single Particle Analysis

2013 ◽  
Vol 135 (39) ◽  
pp. 14528-14531 ◽  
Author(s):  
Andrew P. Ault ◽  
Timothy L. Guasco ◽  
Olivia S. Ryder ◽  
Jonas Baltrusaitis ◽  
Luis A. Cuadra-Rodriguez ◽  
...  
Keyword(s):  
Nitric Acid ◽  
Single Particle ◽  
Aerosol Particles ◽  
Particle Analysis ◽  
Sea Spray ◽  
Single Particle Analysis ◽  
Sea Spray Aerosol

scholarly journals Electron microscopy snapshots of single particles from single cells

2018 ◽  
Vol 294 (5) ◽  
pp. 1602-1608 ◽  
Author(s):  
Xiunan Yi ◽  
Eric J. Verbeke ◽  
Yiran Chang ◽  
Daniel J. Dickinson ◽  
David W. Taylor
Keyword(s):  
Electron Microscopy ◽  
Single Particle ◽  
Protein Complexes ◽  
Signal To Noise Ratio ◽  
Single Cells ◽  
Particle Analysis ◽  
Biological Macromolecules ◽  
Single Particle Analysis ◽  
Whole Cells ◽  
Potential Applications

Cryo-electron microscopy (cryo-EM) has become an indispensable tool for structural studies of biological macromolecules. Two additional predominant methods are available for studying the architectures of multiprotein complexes: 1) single-particle analysis of purified samples and 2) tomography of whole cells or cell sections. The former can produce high-resolution structures but is limited to highly purified samples, whereas the latter can capture proteins in their native state but has a low signal-to-noise ratio and yields lower-resolution structures. Here, we present a simple, adaptable method combining microfluidic single-cell extraction with single-particle analysis by EM to characterize protein complexes from individual Caenorhabditis elegans embryos. Using this approach, we uncover 3D structures of ribosomes directly from single embryo extracts. Moreover, we investigated structural dynamics during development by counting the number of ribosomes per polysome in early and late embryos. This approach has significant potential applications for counting protein complexes and studying protein architectures from single cells in developmental, evolutionary, and disease contexts.

Proton microprobe single particle analysis

1990 ◽  
Vol 21 ◽  
pp. S605-S608 ◽  
Author(s):  
Erik Swietlicki ◽  
Göran Lövestam ◽  
Uwe Wätjen
Keyword(s):  
Single Particle ◽  
Particle Analysis ◽  
Single Particle Analysis ◽  
Proton Microprobe
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