Laboratory toxicity and large-scale commercial validation of the efficacy of ethanedinitrile, a potential alternative fumigant to methyl bromide, to disinfest New Zealand Pinus radiata export logs

2020 ◽  
Vol 88 ◽  
pp. 101671 ◽  
Author(s):  
Adriana J. Najar-Rodriguez ◽  
Saeedeh Afsar ◽  
Kambiz Esfandi ◽  
Matthew K.D. Hall ◽  
Anthony R. Adlam ◽  
...  
2018 ◽  
Vol 71 ◽  
pp. 350
Author(s):  
Adriana J. Najar-Rodriguez ◽  
Matthew K.D. Hall ◽  
Catherine R. Wilks ◽  
Anthony R. Adlam ◽  
Sam Brierley ◽  
...  

The golden-haired bark beetle, Hylurgus ligniperda (F.), is a common forest insect which may be associated with pine (Pinus radiata D.Don) logs exported from New Zealand. We tested the dose-mortality responses of H. ligniperda adults, from two different origins (field vs laboratory), to methyl bromide (MB), the most widely used fumigant worldwide; and to ethanedinitrile (EDN), a potential alternative to MB. Naked insects were fumigated with either MB or EDN at 10°C for 4 and 3 hours, respectively. Laboratory adults had been reared on artificial diet under controlled conditions for >10 generations. Field adults, by contrast, had been recently collected from Lindgren funnel traps with lures of alpha-pinene and ethanol. Tolerance to the two fumigants tested was significantly different, according to the origin of the insects, with field-collected adults being less tolerant to MB and EDN than laboratory-reared ones. The implications of our results for the development of disinfestation schedules for New Zealand export logs will be discussed.    


2013 ◽  
Vol 66 ◽  
pp. 82-88
Author(s):  
K.G. Somerfield ◽  
B.L. Bycroft ◽  
D.W. Brash ◽  
J.N. Clark ◽  
D.I. Hedderley ◽  
...  

Pine (Pinus radiata D Don) logs exported from New Zealand must undergo fumigation to ensure they are free of phytosanitary insects to meet the requirements of importing countries The goldenhaired bark beetle Hylurgus ligniperda (F) is one of the major hitchhiker species that can infest New Zealand pine logs Adults and larvae can be present in the bark and cambium layer of freshly cut logs To examine ways of reducing rates of methyl bromide fumigation diet containing both life stages (extracted from pine logs) was exposed to 0 49 or 73 g/m3 methyl bromide for 16 h in 28litre fumigation chambers at 5 10 15 or 20C Both the 49 g/m3 and 73 g/m3 methyl bromide exposures resulted in 100 mortality at all temperatures Average mortality among controls was larvae 7 adults 23 The results indicate that the present methyl bromide fumigation rates used for pine logs exported from New Zealand could be reduced


2016 ◽  
Vol 69 ◽  
pp. 324-324
Author(s):  
M.K.D. Hall ◽  
A.R. Adlam ◽  
A.J. Hall ◽  
A.J. Najar-Rodriguez

Development of sciencebased fumigation schedules for phytosanitary treatments relies on an understanding of the factors which influence the effectiveness of a fumigant under different conditions Understanding the sorption characteristics of a fumigant is a critical step in establishing commercial treatment schedules as this process which involves adsorption and absorption of fumigant molecules influences the concentration of the fumigant remaining in the treated space after fumigation and hence the amount of fumigant available to kill insects We monitored the concentration of methyl bromide during commercial fumigation of three log stacks (average volume 4078 m3) with 120 g/m3 methyl bromide for 13 h under a tarpaulin Pine (Pinus radiata D Don) logs were fumigated at South Port Bluff (New Zealand) during April 2015 Gas samples from the treated space were taken at 05 1 2 3 the treated space indicating that the rest of the fumigant was sorbed These data can now be combined with insect toxicity studies to guide the development of improved commercial treatment schedules to treat log exports from New Zealand


2016 ◽  
Vol 69 ◽  
pp. 143-152 ◽  
Author(s):  
G.K. Clare ◽  
E.M. George

The goldenhaired bark beetle Hylurgus ligniperda (Coleoptera Scolytidae) is an insect of quarantine concern associated with Pinus radiata in New Zealand Exported logs are fumigated with methyl bromide (MB) before departure or on arrival Research is under way to find alternatives to MB for logs and to manage emissions This research requires large numbers of all stages of H ligniperda to identify effective treatments The study reports the first laboratory method for rearing large numbers of all life stages of H ligniperda A novel oviposition device enabled efficient egg collection Larvae were reared from eggs inoculated directly into artificial diet Life cycle data were obtained for all the developmental stages of H ligniperda Six continuous generations were produced with each generation taking a minimum of 72 days Females laid an average of 30919 eggs each Almost 70 of the eggs formed adults via four larval instars and a pupal stage


2020 ◽  
Vol 89 ◽  
pp. 101718
Author(s):  
Adriana J. Najar-Rodriguez ◽  
Matthew K.D. Hall ◽  
Anthony R. Adlam ◽  
Saeedeh Afsar ◽  
Kambiz Esfandi ◽  
...  

2020 ◽  
Vol 4 (1) ◽  
Author(s):  
Catherine M. Febria ◽  
Maggie Bayfield ◽  
Kathryn E. Collins ◽  
Hayley S. Devlin ◽  
Brandon C. Goeller ◽  
...  

In Aotearoa New Zealand, agricultural land-use intensification and decline in freshwater ecosystem integrity pose complex challenges for science and society. Despite riparian management programmes across the country, there is frustration over a lack in widespread uptake, upfront financial costs, possible loss in income, obstructive legislation and delays in ecological recovery. Thus, social, economic and institutional barriers exist when implementing and assessing agricultural freshwater restoration. Partnerships are essential to overcome such barriers by identifying and promoting co-benefits that result in amplifying individual efforts among stakeholder groups into coordinated, large-scale change. Here, we describe how initial progress by a sole farming family at the Silverstream in the Canterbury region, South Island, New Zealand, was used as a catalyst for change by the Canterbury Waterway Rehabilitation Experiment, a university-led restoration research project. Partners included farmers, researchers, government, industry, treaty partners (Indigenous rights-holders) and practitioners. Local capacity and capability was strengthened with practitioner groups, schools and the wider community. With partnerships in place, co-benefits included lowered costs involved with large-scale actions (e.g., earth moving), reduced pressure on individual farmers to undertake large-scale change (e.g., increased participation and engagement), while also legitimising the social contracts for farmers, scientists, government and industry to engage in farming and freshwater management. We describe contributions and benefits generated from the project and describe iterative actions that together built trust, leveraged and aligned opportunities. These actions were scaled from a single farm to multiple catchments nationally.


2020 ◽  
Vol 22 (1) ◽  
pp. 115-122
Author(s):  
Amarila Malik ◽  
Elita Yuliantie ◽  
Nisa Yulianti Suprahman ◽  
Theresa Linardi ◽  
Angelina Wening Widiyanti ◽  
...  

Background: Bacteriocins (Bac1, Bac2, and Bac3) from Weissella confusa MBF8-1, weissellicin- MBF, have been reported as potential alternative substances as well as complements to the existing antibiotics against many antimicrobial-resistant pathogens. Previously, the genes encoded in the large plasmid, pWcMBF8-1, and the spermicidal activity of their synthetic peptides, originally discovered Indonesia, have been studied. Three synthetic bacteriocins peptides of this weissellicin-MBF have been reported for their potential activities, i.e. antibacterial and spermicidal. Objective: The aim of this study was to construct the recombinant Bacteriocin (r-Bac) genes, as well as to investigate the gene expressions and their functional analysis. Method: Here, the recombinant Bacteriocin (r-Bac) genes were constructed and the recombinant peptides (r-Bac1, r-Bac2, and r-Bac3) in B. subtilis DB403 cells were produced on a large scale. After purification, using the His-tag affinity column, their potential bioactivities were measured as well as their antibacterial minimum inhibitory concentrations against Leuconostoc mesenteroides and Micrococcus luteus, were determined. Results: Pure His-tag-recombinant Bac1, Bac2, and Bac3 were obtained and they could inhibit the growth of L. mesenteroides and M. luteus. Conclusion: The recombinant bacteriocin could be obtained although with weak activity in inhibiting gram-positive bacterial growth.


2004 ◽  
Vol 31 (3) ◽  
pp. 319 ◽  
Author(s):  
Jane Catherine Kitson

Sooty shearwaters (tītī, muttonbird, Puffinus griseus) are highly abundant migratory seabirds, which return to breeding colonies in New Zealand. The Rakiura Māori annual chick harvest on islands adjacent to Rakiura (Stewart Island), is one of the last large-scale customary uses of native wildlife in New Zealand. This study aimed to establish whether the rate at which muttonbirders can extract chicks from their breeding burrows indicates population trends of sooty shearwaters. Harvest rates increased slightly with increasing chick densities on Putauhinu Island. Birders' harvest rates vary in their sensitivities to changing chick density. Therefore a monitoring panel requires careful screening to ensure that harvest rates of the birders selected are sensitive to chick density, and represents a cross-section of different islands. Though harvest rates can provide only a general index of population change, it can provide an inexpensive and feasible way to measure population trends. Detecting trends is the first step to assessing the long-term sustainability of the harvest.


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