Separation and purification of indigotin and indirubin from Folium isatidis extracts using a fast and efficient macroporous resin column followed reversed phase flash chromatography

2016 ◽  
Vol 67 ◽  
pp. 61-68 ◽  
Author(s):  
Yu-Yan Huang ◽  
Xue-Feng Liu ◽  
Ju-Zhao Liu ◽  
Lu Li ◽  
Qi Cui ◽  
...  
Keyword(s):  
Reversed Phase ◽  
Flash Chromatography ◽  
Separation And Purification ◽  
Macroporous Resin ◽  
Resin Column

scholarly journals Separation and Purification of Zizyphusine, Spinosin, and 6′′′-Feruloylspinosin From Zizyphi Spinosi Semen

2020 ◽  
Vol 15 (3) ◽  
pp. 1934578X2091055
Author(s):  
Jiong Ran Wang ◽  
Yan Zhou Hu ◽  
Xiao Han ◽  
Ke Ding
Keyword(s):  
High Pressure ◽  
Physiological Function ◽  
Ultrasonic Extraction ◽  
Flash Chromatography ◽  
Preparative Chromatography ◽  
Separation And Purification ◽  
Macroporous Resin ◽  
Extraction Separation ◽  
Resin Separation

The methods of extraction, separation, and purification of zizyphusine, spinosin, and 6′′′-feruloylspinosin from Zizyphi Spinosi Semen ( ZSS) were investigated. From 31.2 g defatted ZSS, 47.7 mg zizyphusine, 57.8 mg spinosin, and 80.5 mg 6′′′-feruloylspinosin were obtained after ultrasonic extraction, purification with macroporous resin, separation by flash chromatography, and purification by high-pressure preparative chromatography. The purities of zizyphusine, spinosin, and 6′′′-feruloylspinosin were 98.6%, 98.2%, and 99.0%, respectively, and their yields 85.0%, 82.8%, and 83.0%. The methods provide stable samples of these compounds for further study of their physiological function and application.

scholarly journals Simultaneous isolation and purification of cucurbitacin D and I from Ecballium elaterium (l.) A. Rich fruit juice

2019 ◽  
Vol 38 (2) ◽  
pp. 171
Author(s):  
Emir Tosun ◽  
Ahmet Baysar
Keyword(s):  
Fruit Juice ◽  
Chloroform Extract ◽  
Reversed Phase ◽  
Hplc Method ◽  
Uv Spectra ◽  
Flash Chromatography ◽  
Separation And Purification ◽  
Spectra Analysis ◽  
Isolation And Purification ◽  
Cucurbitacin D

The objective of this study was to develop a rapid, economic, and efficient method for simultaneous selective isolation, separation, and purification of cucurbitacin D and I from Ecballium elaterium (L.) A. Rich fruit juice via reversed-phase flash chromatography combined with HPLC. The chloroform extract of the fruit juice was fractionated with flash chromatography using a chloroform, acetone and methanol solvent combination at a 5 ml/min flow rate. Then, a validated HPLC method was utilized for purification of the two targeted cucurbitacins. Cucurbitacin D and I were collected automatically by the fraction collector. The fractions containing the same compounds were pooled and lyophilized. The purified cucurbitacin D and I compounds were identified by NMR, LC-MS, and UV spectra analysis. The results suggest that the applied procedure is simple, quick, and highly efficient. The HPLC method was found to be linear, accurate, precise and rugged for the quantification of the cucurbitacins studied.

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