High purity separation of hypericin from Hypericum perforatum L. extract with macroporous resin column coupling preparative liquid chromatography

2021 ◽  
Author(s):  
Jie Zhang ◽  
Chunrong Feng ◽  
Pu Ge ◽  
Qingqing Wang ◽  
Yanling Liu ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Purity ◽  
Hypericum Perforatum ◽  
Macroporous Resin ◽  
Resin Column ◽  
Preparative Liquid Chromatography ◽  
Hypericum Perforatum L ◽  
Column Coupling

scholarly journals Simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with 7,12-dimethylbenz[a]anthracene and Hypericum Perforatum L. by high-performance liquid chromatography with diode-array detection

2010 ◽  
Vol 8 (1) ◽  
pp. 108-115 ◽  
Author(s):  
Abdulkadir Levent ◽  
Suat Ekin ◽  
Gökhan Oto
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hypericum Perforatum ◽  
Treated Group ◽  
Retinyl Palmitate ◽  
Rat Serum ◽  
Array Detection ◽  
Hypericum Perforatum L ◽  
Β Carotene

AbstractA new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate and β-carotene in rat serum treated with Hypericum Perforatum L. and 7,12-dimethylbenz[a]anthracene. Furthermore, vitamin C was determined spectrophotometrically. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-acetonitrile-tetrahydrofuran (65:30:5, v/v/v) as mobile phase, at a flow rate of 1.5 mL min−1 and 40°C. Diode-array detection was conducted at 325 and 450 nm for retinol and retinyl palmitate, and β-carotene, respectively with a running time of 26 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. This method was then applied to determine the amounts of retinol, retinyl palmitate and β-carotene in rat serum. Results of this study demonstrated that at 60th day in the 7,12-dimethylbenz[a]anthracene-treated group there was a significant decrease (pa] anthracene + Hypericum Perforatum L. treated group compared to the control group..

scholarly journals Isolation of High Purity Anthocyanin Monomers from Red Cabbage with Recycling Preparative Liquid Chromatography and Their Photostability

Molecules ◽  
2018 ◽  
Vol 23 (5) ◽  
pp. 991 ◽  
Author(s):  
Yijun Chen ◽  
Zikun Wang ◽  
Hanghang Zhang ◽  
Yuan Liu ◽  
Shuai Zhang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
High Purity ◽  
Preparative Liquid Chromatography ◽  
Red Cabbage

scholarly journals Simultaneous Determination of the Predominant Hyperforins and Hypericins in St. John's Wort (Hypericum perforatum L.) by Liquid Chromatography

2000 ◽  
Vol 83 (4) ◽  
pp. 944-949 ◽  
Author(s):  
Dean E Gray ◽  
George E Rottinghaus ◽  
H E Gene Garrett ◽  
Stephen G Pallardy
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Hypericum Perforatum ◽  
Dry Weight ◽  
Active Constituents ◽  
St John’S Wort ◽  
St John's Wort ◽  
Relative Standard ◽  
Hypericum Perforatum L

Abstract Hypericin and hyperforin are believed to be among the active constituents in common St. John's wort (Hypericum perforatum L.). Presently, dietary supplements are generally standardized to contain specified levels of hypericin and hyperforin, and the related compounds, pseudohypericin and adhyperforin. A rapid method was developed for simultaneous determination of these 4 active constituents by liquid chromatography (LC). A 1 g portion of dried, finely ground leaf/flower sample is extracted with 20 mL methanol for 2 h. A 0.6 mL aliquot of the crude extract is combined with 5.4 mL acetonitrile–methanol (9 + 1) and passed through a mixed solid-phase cleanup column. The eluate is examined by LC for hyperforin, adhyperforin, hypericin, and pseudohypericin on a Hypersil reversed-phase column by using simultaneous ultraviolet (284 nm) and fluorescence detection (excitation, 470 nm; emission, 590 nm). The compounds are easily separated isocratically within 8 min with a mobile phase of acetonitrile–aqueous 0.1M triethylammonium acetate (8 + 2). Average recoveries of hyperforin and adhyperforin were 101.9 and 98.4%, respectively, for 3 sample mixtures containing concentrations ranging from approximately 0.2 to 1.5% combined hyperforins per gram dry weight. Average relative standard deviation (RSD) values for hyperforin and adhyperforin for all 3 mixtures were 18.9 and 18.0%, respectively. Average recoveries of hypericin and pseudohypericin were 88.6 and 93.3% respectively, from 3 sample mixtures containing concentrations ranging from approximately 0.2 to 0.4% combined hypericins per gram dry weight. Average RSD values for hypericin and pseudohypericin for all 3 mixtures were 3.8 and 4.2%, respectively.

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