scholarly journals Separation and Purification of Zizyphusine, Spinosin, and 6′′′-Feruloylspinosin From Zizyphi Spinosi Semen

2020 ◽  
Vol 15 (3) ◽  
pp. 1934578X2091055
Author(s):  
Jiong Ran Wang ◽  
Yan Zhou Hu ◽  
Xiao Han ◽  
Ke Ding
Keyword(s):  
High Pressure ◽  
Physiological Function ◽  
Ultrasonic Extraction ◽  
Flash Chromatography ◽  
Preparative Chromatography ◽  
Separation And Purification ◽  
Macroporous Resin ◽  
Extraction Separation ◽  
Resin Separation

The methods of extraction, separation, and purification of zizyphusine, spinosin, and 6′′′-feruloylspinosin from Zizyphi Spinosi Semen ( ZSS) were investigated. From 31.2 g defatted ZSS, 47.7 mg zizyphusine, 57.8 mg spinosin, and 80.5 mg 6′′′-feruloylspinosin were obtained after ultrasonic extraction, purification with macroporous resin, separation by flash chromatography, and purification by high-pressure preparative chromatography. The purities of zizyphusine, spinosin, and 6′′′-feruloylspinosin were 98.6%, 98.2%, and 99.0%, respectively, and their yields 85.0%, 82.8%, and 83.0%. The methods provide stable samples of these compounds for further study of their physiological function and application.

Study on Macroporous Resin Separation and Purification of Total Flavonoids of Plantago Process

2012 ◽  
Vol 550-553 ◽  
pp. 987-992 ◽  
Author(s):  
Yong Guang Bi ◽  
Yao Quan Tan
Keyword(s):  
Volume Fraction ◽  
Stable Process ◽  
Process Conditions ◽  
Total Flavonoids ◽  
Separation And Purification ◽  
Optimum Conditions ◽  
Flavonoid Content ◽  
Macroporous Resin ◽  
Crude Drug ◽  
Resin Separation

To study the macroporous resin separation and purification of total flavonoids of Plantago optimum conditions, the static adsorption and desorption experiments by filtering out the best macroporous resin, and plantain extract for the assessment of total flavonoid content indicators in the single-factor test based on the orthogonal design, the amount of resin in the hole, eluent concentration, alcohol consumption and other factors for the study to optimize the AB-8 macroporous resin separation and purification of total flavonoids plantain best process conditions. The results show that: the effect of various factors affecting the size of the order of separation and purification has to: eluent concentration> ethanol consumption> macroporous resin usage, optimization of the optimum conditions: resin dosage is 1 times the amount of crude drug, the use of eluent volume fraction of 40% ethanol, elution agent is 12.5 times the amount of crude drug. Verify the conditions in this test, three repeated experiments were similar, the average flavonoid content 0.2127%, the transfer rate of 90.57%, indicating that the scientific and rational and stable process conditions can be effectively used plantain purified flavonoids.

scholarly journals Liquid Biphasic System: A Recent Bioseparation Technology

Processes ◽  
2020 ◽  
Vol 8 (2) ◽  
pp. 149 ◽  
Author(s):  
Kuan Shiong Khoo ◽  
Hui Yi Leong ◽  
Kit Wayne Chew ◽  
Jun-Wei Lim ◽  
Tau Chuan Ling ◽  
...  
Keyword(s):  
Future Development ◽  
Future Prospect ◽  
Fundamental Principle ◽  
Present Review ◽  
Biphasic System ◽  
Separation And Purification ◽  
Separation Techniques ◽  
System A ◽  
Extraction Separation ◽  
Further Development

A well-known bioseparation technique namely liquid biphasic system (LBS) has attracted many researchers’ interest for being an alternative bioseparation technology for various kinds of biomolecules. The present review begins with an in-depth discussion on the fundamental principle of LBS and this is followed by the discussion on further development of various phase-forming components in LBS. Additionally, the implementation of various advance technologies to the LBS that is beneficial towards the efficiency of LBS for the extraction, separation, and purification of biomolecules was discussed. The key parameters affecting the LBS were presented and evaluated. Moreover, future prospect and challenges were highlighted to be a useful guide for future development of LBS. The efforts presented in this review will provide an insight for future researches in liquid-liquid separation techniques.

Separation and purification of l-methionine from E. coli fermentation broth by macroporous resin chromatography

2019 ◽  
Vol 1110-1111 ◽  
pp. 108-115 ◽  
Author(s):  
Neng Xiong ◽  
Ru Yu ◽  
Tao Chen ◽  
Ya-Ping Xue ◽  
Zhi-Qiang Liu ◽  
...  
Keyword(s):  
Fermentation Broth ◽  
Separation And Purification ◽  
Macroporous Resin ◽  
E Coli

scholarly journals Technologies for the Extraction, Separation and Purification of polyphenols – A Review

2019 ◽  
Vol 6 (1) ◽  
pp. 74-91 ◽  
Author(s):  
Shyam Suwal ◽  
Alice Marciniak
Keyword(s):  
Organic Molecules ◽  
Extraction Efficiency ◽  
Positive Impact ◽  
Cost Effective ◽  
Separation And Purification ◽  
Advantages And Disadvantages ◽  
Assisted Extraction ◽  
Extraction Separation ◽  
Current Context ◽  
Conventional Extraction

Polyphenols are high molecular weight, organic molecules mainly found in plant kingdom. They are mostly known for their positive impact on health, specifically for their antioxidant activity. Indeed, they are widely studied for the prevention of multiple diseases such as cancer, inflammatory, cardiovascular and neurodegenerative diseases. Nevertheless, extractions of these growing interest molecules remain challenging using conventional methods such as solvent extraction. That is why recent researches have focused on improving the extraction of polyphenol by using different technologies such as ultrasound, microwave, pressurized liquid, pulsed electric field, supercritical fluid and high hydrostatic pressure. In the current context, the assisted-extraction should demonstrate their potential to improve the extraction efficiency while being cost-effective and with a low environmental impact. To this end, technologies ought to, for instance, increase the solubility of polyphenol and the permeability of the cell wall. Consequently, this review is focused on the use and potential of these technologies to improve polyphenol extractions from plants as well as their purification using various methods. It discusses of the advantages and disadvantages with some examples of all these technologies assisted-extraction in comparison with conventional extraction method as well as purification technology.

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