257 FATE OF STEM CELLS IN INJURY: IN-VIVO REAL TIME TRACKING OF MESENCHYMAL STEM CELLS (MSCS) IN A RAT MODEL OF STRESS URINARY INCONTINENCE (SUI)

Female stress urinary incontinence (FSUI) is prevalent in women with type 2 diabetes/obesity (T2D/O), and treatment is not optimal. Autograph stem cell therapy surprisingly has poor efficacy. In the male rat model of T2D/O, it was demonstrated that epigenetic changes, triggered by long-term exposure to the dyslipidemic milieu, led to abnormal global transcriptional signatures (GTS) of genes and microRNAs (miR), and impaired the repair capacity of muscle-derived stem cells (MDSC). This was mimicked in vitro by treatment of MDSC with dyslipidemic serum or lipid factors. The current study aimed to predict whether these changes also occur in stem cells from female 12 weeks old T2D/O rats, a model of FSUI. MDSCs from T2D/O (ZF4-SC) and normal female rats (ZL4-SC) were treated in vitro with either dyslipidemic serum (ZFS) from late T2D/O 24 weeks old female Zucker fatty (ZF) rats, or normal serum (ZLS) from 24 weeks old female Zucker lean (ZL) rats, for 4 days and subjected to assays for fat deposition, apoptosis, scratch closing, myostatin, interleukin-6, and miR-GTS. The dyslipidemic ZFS affected both female stem cells more severely than in the male MDSC, with some gender-specific differences in miR-GTS. The changes in miR-GTS and myostatin/interleukin-6 balance may predict in vivo noxious effects of the T2D/O milieu that might impair autograft stem cell (SC) therapy for FSUI, but this requires future studies.

Download Full-text

Correction: In vivo migration of Fe3O4@polydopamine nanoparticle-labeled mesenchymal stem cells to burn injury sites and their therapeutic effects in a rat model

Biomaterials Science ◽

10.1039/d0bm90110e ◽

2021 ◽

Author(s):

Xiuying Li ◽

Zhenhong Wei ◽

Binxi Li ◽

Jing Li ◽

Huiying Lv ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Rat Model ◽

Burn Injury ◽

Therapeutic Effects

Correction for ‘In vivo migration of Fe3O4@polydopamine nanoparticle-labeled mesenchymal stem cells to burn injury sites and their therapeutic effects in a rat model’ by Xiuying Li et al., Biomater. Sci., 2019, 7, 2861–2872, DOI: 10.1039/C9BM00242A.

Download Full-text

MYOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO AND LONG-TERM SURVIVAL IN A NUDE RAT MODEL FOR URINARY INCONTINENCE

The Journal of Urology ◽

10.1016/s0022-5347(09)61907-8 ◽

2009 ◽

Vol 181 (4) ◽

pp. 681

Author(s):

Gerhard Feil ◽

Adriana Drost ◽

Simon Baumann ◽

Jochen Schäfer ◽

Sibylle Weng ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Urinary Incontinence ◽

Rat Model ◽

Myogenic Differentiation ◽

Human Mesenchymal Stem Cells ◽

Term Survival ◽

Nude Rat

Download Full-text

Injectable Hydrogel Combined with Nucleus Pulposus-Derived Mesenchymal Stem Cells for the Treatment of Degenerative Intervertebral Disc in Rats

Stem Cells International ◽

10.1155/2019/8496025 ◽

2019 ◽

Vol 2019 ◽

pp. 1-17 ◽

Cited By ~ 5

Author(s):

Feng Wang ◽

Li-ping Nan ◽

Shi-feng Zhou ◽

Yang Liu ◽

Ze-yu Wang ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Intervertebral Disc ◽

Nucleus Pulposus ◽

Rat Model ◽

Injectable Hydrogel ◽

Cell Scaffold ◽

Ivd Degeneration

Stem cell-based tissue engineering in treating intervertebral disc (IVD) degeneration is promising. An appropriate cell scaffold can maintain the viability and function of transplanted cells. Injectable hydrogel has the potential to be an appropriate cell scaffold as it can mimic the condition of the natural extracellular matrix (ECM) of nucleus pulposus (NP) and provide binding sites for cells. This study was aimed at investigating the effect of injectable hydrogel-loaded NP-derived mesenchymal stem cells (NPMSC) for the treatment of IVD degeneration (IDD) in rats. In this study, we selected injectable 3D-RGD peptide-modified polysaccharide hydrogel as a cell transplantation scaffold. In vitro, the biocompatibility, microstructure, and induced differentiation effect on NPMSC of the hydrogel were studied. In vivo, the regenerative effect of hydrogel-loaded NPMSC on degenerated NP in a rat model was evaluated. The results showed that NPMSC was biocompatible and able to induce differentiation in hydrogel in vivo. The disc height index (almost 87%) and MRI index (3313.83±227.79) of the hydrogel-loaded NPMSC group were significantly higher than those of other groups at 8 weeks after injection. Histological staining and immunofluorescence showed that the hydrogel-loaded NPMSC also partly restored the structure and ECM content of degenerated NP after 8 weeks. Moreover, the hydrogel could support long-term NPMSC survival and decrease cell apoptosis rate of the rat IVD. In conclusion, injectable hydrogel-loaded NPMSC transplantation can delay the level of IDD and promote the regeneration of the degenerative IVD in the rat model.

Download Full-text

Regenerative potential of human dental pulp stem cells in the treatment of stress urinary incontinence: In vitro and in vivo study

Cell Proliferation ◽

10.1111/cpr.12675 ◽

2019 ◽

Vol 52 (6) ◽

Cited By ~ 4

Author(s):

Alessio Zordani ◽

Alessandra Pisciotta ◽

Laura Bertoni ◽

Giulia Bertani ◽

Antonio Vallarola ◽

...

Keyword(s):

Stem Cells ◽

Urinary Incontinence ◽

Stress Urinary Incontinence ◽

Dental Pulp ◽

Dental Pulp Stem Cells ◽

In Vivo Study ◽

Human Dental Pulp

Download Full-text

Combined Treatment With CCR1-Overexpressing Mesenchymal Stem Cells and CCL7 Enhances Engraftment and Promotes the Recovery of Simulated Birth Injury-Induced Stress Urinary Incontinence in Rats

Frontiers in Surgery ◽

10.3389/fsurg.2020.00040 ◽

2020 ◽

Vol 7 ◽

Author(s):

Hai-Hong Jiang ◽

Ling-Xiao Ji ◽

Hai-Yan Li ◽

Qi-Xiang Song ◽

Yasmeen Bano ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Urinary Incontinence ◽

Stress Urinary Incontinence ◽

Combined Treatment ◽

Birth Injury ◽

Induced Stress

Download Full-text

In vivo real-time visualization of mesenchymal stem cells tropism for cutaneous regeneration using NIR-II fluorescence imaging

Biomaterials ◽

10.1016/j.biomaterials.2015.02.090 ◽

2015 ◽

Vol 53 ◽

pp. 265-273 ◽

Cited By ~ 57

Author(s):

Guangcun Chen ◽

Fei Tian ◽

Chunyan Li ◽

Yejun Zhang ◽

Zhen Weng ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Real Time ◽

Fluorescence Imaging ◽

Real Time Visualization

Download Full-text

Effects of Magnetically Guided, SPIO-Labeled, and Neurotrophin-3 Gene-Modified Bone Mesenchymal Stem Cells in a Rat Model of Spinal Cord Injury

Stem Cells International ◽

10.1155/2016/2018474 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 6

Author(s):

Rui-Ping Zhang ◽

Ling-Jie Wang ◽

Sheng He ◽

Jun Xie ◽

Jian-Ding Li

Keyword(s):

Spinal Cord ◽

Stem Cells ◽

Spinal Cord Injury ◽

Mesenchymal Stem Cells ◽

Rat Model ◽

Therapeutic Effects ◽

Magnetic Targeting ◽

Bone Mesenchymal Stem Cells ◽

Cord Injury

Despite advances in our understanding of spinal cord injury (SCI) mechanisms, there are still no effective treatment approaches to restore functionality. Although many studies have demonstrated that transplantingNT3gene-transfected bone marrow-derived mesenchymal stem cells (BMSCs) is an effective approach to treat SCI, the approach is often low efficient in the delivery of engrafted BMSCs to the site of injury. In this study, we investigated the therapeutic effects of magnetic targeting ofNT3gene-transfected BMSCs via lumbar puncture in a rat model of SCI. With the aid of a magnetic targeting cells delivery system, we can not only deliver the engrafted BMSCs to the site of injury more efficiently, but also perform cells imaging in vivo using MR. In addition, we also found that this composite strategy could significantly improve functional recovery and nerve regeneration compared to transplantingNT3gene-transfected BMSCs without magnetic targeting system. Our results suggest that this composite strategy could be promising for clinical applications.

Download Full-text