scholarly journals Development of a real time reverse transcription loop-mediated isothermal amplification method (RT-LAMP) for detection of a novel swine acute diarrhea syndrome coronavirus (SADS-CoV)

2018 ◽  
Vol 260 ◽  
pp. 45-48 ◽  
Author(s):  
Huanan Wang ◽  
Feng Cong ◽  
Fanwen Zeng ◽  
Yuexiao Lian ◽  
Xiangnan Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Acute Diarrhea ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method ◽  
Diarrhea Syndrome

scholarly journals Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of SARS-CoV-2

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9278 ◽  
Author(s):  
Yee Ling Lau ◽  
Ilyiana Ismail ◽  
Nur Izati Mustapa ◽  
Meng Yee Lai ◽  
Tuan Suhaila Tuan Soh ◽  
...  
Keyword(s):  
Real Time ◽  
Reverse Transcription ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Clinical Sensitivity ◽  
Loop Mediated Isothermal Amplification ◽  
Amplification Method ◽  
Resource Limited ◽  
Highly Sensitive ◽  
Comparable Performance

Background Highly sensitive real-time reverse transcription polymerase chain reaction (RT-qPCR) methods have been developed for the detection of SARS-CoV-2. However, they are costly. Loop-mediated isothermal amplification (LAMP) assay has emerged as a novel alternative isothermal amplification method for the detection of nucleic acid. Methods A rapid, sensitive and specific real-time reverse transcription LAMP (RT-LAMP) assay was developed for SARS-CoV-2 detection. Results This assay detected one copy/reaction of SARS-CoV-2 RNA in 30 min. Both the clinical sensitivity and specificity of this assay were 100%. The RT-LAMP showed comparable performance with RT-qPCR. Combining simplicity and cost-effectiveness, this assay is therefore recommended for use in resource resource-limited settings.

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